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Abdulhasan J.M.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2014

Klebsiella pneumonia carbapenemases (KPCs) are class A variant of β -lactamase enzymes capable of hydrolyzing all known β -lactam antibiotics. Worldwide spread of KPC producing strain makes them a potential threat for current antibiotic based therapy. In this study, we isolated the K. pneumoniae from the urine samples and screened for antibiotic resistance. Then molecular amplification techniques PCR was used for identification of KPC gene in these isolated samples. The PCR results coordinate with the antibiotic susceptibility testing and KPC positive isolates showed a multidrug resistant. Considering the potential for rapid horizontal and vertical transmission of KPC genes, prompt recognition is critical. This study validated a rapid, sensitive, and specific PCR assay for the detection of KPC gene.

Pushpa N.,Bangalore University | Kokila M.K.,Bangalore University | Shivaramu N.J.,Indian Academy Degree College
Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms | Year: 2016

Undoped and Eu3+ doped La2O3 nanophosphor are synthesized by low temperature sol-gel technique. The synthesized samples are characterized by X-ray diffraction (XRD) and average crystallite size is found to be ∼18 nm and ∼23 nm for undoped and Eu3+ doped La2O3 respectively. Gamma ray irradiated undoped La2O3 shows high intense thermoluminescence (TL) glow peak at 640 K and weak TL glow peak at 443 K and the high intense peak intensity is sub linear increase with γ-dose. Whereas Eu3+ doped La2O3 nanophosphor show a prominent TL glow peak at 640 K and its TL intensity linearly increases up to 1 kGy. The kinetic parameters are estimated using glow curve deconvoluted (GCD) technique. TL emission of γ-ray irradiated Eu3+ doped La2O3 show peaks at 508, 586, 619 and 706 nm are attributed to Eu3+ transition peaks. © 2016 Elsevier B.V. All rights reserved.

Abbodi M.A.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2013

In the present study I isolated breast cancer stem cells from cell line MDA-MB231 by used CD44 MicroBeads separator. Cancer stem cells (CSCs) were identified by used PCR machine; here specific primers were designed for CD44 and CD24 genes, whereas CD44 positive for breast CSCs and CD24 negative CSCs. MDA-MB231 cells and breast CSCs treated with G47Δ vector. G47Δ showed highly effect on both kinds of cells by killing over 90% of MDA-MB231 and over 80% of the CSCs in vitro. Virus used as therapy demonstrated that pathogenic microorganisms can be modify genetically and use for targeting diseases and oHSV can be used for treatment breast CSCs that are still not understood. This study demonstrates that oHVS effective against breast cancer stem cells and could be a beneficial method for treating cancer stem cells expressed in breast cancer.

Dheyab A.S.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2013

Infections caused by antibiotic-resistant bacteria result in higher mortality and antibiotic resistance are a recognized major public health threat. S. aureus is a gram-positive microorganism with numerous virulence factors having the ability to acquire antibiotic resistance from the environment. The pathogenic S. aureus were isolated from hospital samples and indentified on the basis of morphological and biochemical characteristics. The antibiotic resistance pattern of these samples was determined by disc diffusion method against 10 antibiotics (Amoxicillin, Gentamicin, Methionine, Cloxacillin, Fluconazol, Ampicillin, Rifampcin, Tetracycline, Azithromycin and Cefixime). Furthermore, DNA fingerprint was performed for these samples using Random primers. Then dendrogram analysis was done to determine the genomic similarity of these samples.

Mohsen A.M.A.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2013

India has one of the largest pools of hepatitis B-infected patients. The presence of HBs Ag in serum or plasma is an indication of active Hepatitis B infection either acute or chronic. Quantitative detection of Hepatitis B virus (HBV) DNA in serum by real time polymerase chain reaction (PCR) assay emerged as a gold standard in guided anti viral therapy. Evidences are suggestive of association of HBV genotype in liver dysfunction. We evaluated the performance as well as usefulness of both ELISA and PCR for detection of the major blood borne pathogen, HBV in southern India. First ALT and AST enzymes level was determined to check liver function. Serum of patients with chronic hepatitis B (HBsAg positive) and healthy individuals were tested both quantitatively and qualitatively. 4.5% of participants were HBsAg Positive by ELISA, while 7.5% of participants showed HBV DNA in real time PCR.

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