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Mehran M.J.,Indian Academy Degree College | Zendehbad S.H.,Indian Academy Degree College | Malla S.,Indian Academy Degree College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

To isolate and quantify the protein and reducing sugar content of the cassava roots together with the antioxidant potential assays, which also included the inhibitory values on free radicals. The cassava roots were pulverized and extracted with both aqueous and organic extraction phases. The total protein content and reducing sugars and the flavonoid contents were estimated as per the protocol for all the four extract fractions. In addition, the free radical scavenging activity and SOD assay was done on all the four extracts. The total polyphenol content and the antioxidant potential assay was also estimated and to correlate the positive relation between the polyphenol content and the antioxidant power. The aqueous extract showed more content of reducing sugars and total protein content and flavonoids. The free radical scavenging activity was also more for the aqueous extract. In contrary the SOD activity was more towards the hexane fraction than the other three extraction phases. There is also positive correlation observed between the polyphenol content and the antioxidant power of the extracts. The cassava, a staple food though lacks the vitamin C content and is responsible for malnutrition among the consumers, it still has a good load of antioxidants which can make the plant a natural protective towards stress related diseases.

Abdulhasan J.M.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2014

Klebsiella pneumonia carbapenemases (KPCs) are class A variant of β -lactamase enzymes capable of hydrolyzing all known β -lactam antibiotics. Worldwide spread of KPC producing strain makes them a potential threat for current antibiotic based therapy. In this study, we isolated the K. pneumoniae from the urine samples and screened for antibiotic resistance. Then molecular amplification techniques PCR was used for identification of KPC gene in these isolated samples. The PCR results coordinate with the antibiotic susceptibility testing and KPC positive isolates showed a multidrug resistant. Considering the potential for rapid horizontal and vertical transmission of KPC genes, prompt recognition is critical. This study validated a rapid, sensitive, and specific PCR assay for the detection of KPC gene.

Irayyif S.M.,Indian Academy Degree College | Senthil Kumar R.,Indian Academy Degree College | Malla S.,Indian Academy Degree College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Methicillin-resistant Staphylococcus aureus is one of the most important causes of hospital infections worldwide. High-level resistance to methicillin is caused by the mecA gene, which encodes an alternative penicillin-binding protein. S. aureus is an important cause of bloodstream infections and a leading cause of severe health care-Associated infections. Laboratory diagnostics S. aureus is a Gram-positive, catalase positive aerobic or anaerobic coccus showing hemolytic and large yellow colonies. S. aureus strains resistant to methicillin and many other antibiotics are major causes of nosocomial infections worldwide. It is of great necessity to check for those genes and check for the potential to clone partial or major fragments into vectors, which can be a potential candidate for DNA vaccines. Novel methods are being developed for the production of antibodies to specific antigens and thus helping in the process of development of protein-based vaccines. mecA (methicillin-resistant) gene was isolated and ligated into pTZ57R/T cloning vector. The ligated product was then cloned into DH5α strain and allowed to propagate. The plasmids thus cloned were purified and later expressed for the gene of interest in an expression vector. The proteins specific to the gene of interest was then isolated and purified. This proteins purified can in turn be used for protein-based vaccines. © 2014, Asian J Pharm Clin Res. All right reserved.

Sibi G.,Indian Academy Degree College | Anuraag T.S.,Indian Academy Degree College | Bafila G.,Indian Academy Degree College
OnLine Journal of Biological Sciences | Year: 2014

Higher photosynthetic efficiency and biomass production with rapid growth makes microalgae as potential candidates over other energy crops in many applications. Heavy metals influence the production of secondary metabolites and lipd content of microalgae in particular. A study was conducted using six Chlorella species under heavy metal exposure to evaluate the copper stress on biomass, cellular and lipid contents. Preliminary growth studies indicated the growth tolerance levels of Chlorella in the presence of copper at 4.0 mg L-1 concentration. The total chlorophyll, protein and lipid content of the isolates were 1.7-3.45%, 0.43-0.70 mg g-1 and 0.02-0.11 mg g-1 respectively. Gas Chromatography-Mass Spectroscopy analysis revealed that the percent composition of fatty acids varied among the species studied and the major group of fatty acids were C16:0, C18:1 and C18:2. Highest percent of fatty acids were found in C. vulgaris, C. protothecoides and C. pyrenoidosa. Copper have an impact on Chlorella species where biomass content was directly proportional to the lipid productivity. The results reflects the fact that copper stress on Chlorella species as the evidence of lipid production in both qualitative and quantitative manner. In conclusion, Chlorella species can be used for the sustainable producion of renewable energy through copper stress and removal of copper from aqueous solutions. © 2014 G. Sibi et al.

Salman H.A.,Indian Academy Degree College | Arjunan S.,Indian Academy Degree College | Senthilkumar R.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2014

Since the mono gastric animals are not capable of metabolizing phytate, a rich source of Phosphorus, undigested phytate is excreted in manure without absorbed for metabolism and poses serious phosphorus pollution in the environment. Therefore, supplementation of phytases into animal feed is expected to solve the above problems. The present investigation deals with cloning and expression of a phytase-PhyL gene from Bacillus licheniformisin in E. coli strain DH5 alpha to facilitate large scale production of this enzyme. DNA was isolated from Bacillus licheniformis ATCC14580 and the phytase-PhyL gene was amplified. The amplified gene was cloned in ready to ligate cloning vector and cloning was confirmed by colony PCR and plasmid digestion. The reported and confirmed clone in this study will be useful in resolving the possible extensive production of recombinant phytases by means of fermentation. This cost effective phytases can be utilized as a feed supplement in animals.

Mohammed H.B.,Indian Academy Degree College | Senthil Kumar R.,Indian Academy Degree College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

Although disinfection methods currently used in drinking water treatment can effectively control microbial pathogens, research in the past few decades have revealed a dilemma between effective disinfection and formation of harmful and to consider innovative approaches that enhance the effectiveness. With the rapid advancement of nanoscience and nanotechnology, detailed knowledge of interactions between engineered nanomaterials and cells, tissues and organisms have become increasingly important, especially in regard to possible hazards to human health. The study was mainly designed to study the effect of silver and gold nanoparticles against the enterococcal pathogens. The organisms were also tested against the antibiotics to prove of their susceptibility toward the drugs. The enterococcal pathogens studied here showed a positive response for all the drugs used. The silver and gold nanoparticles are studied in this context to prove of their effect on the bacterial pathogens. Gold and silver particles are studied separately and are found to show some significant effect on the growth of bacteria. The pathogens studied showed susceptibility toward both the particles (gold and silver). And the effect of the nanoparticles was completely dose dependent, i.e., the effect was found to be more at higher concentration. All effects were statistically significant at the 0.05 significance level. There was a significant effect of the nanoparticles (gold and silver) among the four different concentrations remembered at the p<0.05 level. Gold nanoparticles showed a significance to the concentration (F [1,7]=13.36364, p=0.035353). © 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.

Pushpa N.,Bangalore University | Kokila M.K.,Bangalore University | Shivaramu N.J.,Indian Academy Degree College
Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms | Year: 2016

Undoped and Eu3+ doped La2O3 nanophosphor are synthesized by low temperature sol-gel technique. The synthesized samples are characterized by X-ray diffraction (XRD) and average crystallite size is found to be ∼18 nm and ∼23 nm for undoped and Eu3+ doped La2O3 respectively. Gamma ray irradiated undoped La2O3 shows high intense thermoluminescence (TL) glow peak at 640 K and weak TL glow peak at 443 K and the high intense peak intensity is sub linear increase with γ-dose. Whereas Eu3+ doped La2O3 nanophosphor show a prominent TL glow peak at 640 K and its TL intensity linearly increases up to 1 kGy. The kinetic parameters are estimated using glow curve deconvoluted (GCD) technique. TL emission of γ-ray irradiated Eu3+ doped La2O3 show peaks at 508, 586, 619 and 706 nm are attributed to Eu3+ transition peaks. © 2016 Elsevier B.V. All rights reserved.

Abbodi M.A.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2013

In the present study I isolated breast cancer stem cells from cell line MDA-MB231 by used CD44 MicroBeads separator. Cancer stem cells (CSCs) were identified by used PCR machine; here specific primers were designed for CD44 and CD24 genes, whereas CD44 positive for breast CSCs and CD24 negative CSCs. MDA-MB231 cells and breast CSCs treated with G47Δ vector. G47Δ showed highly effect on both kinds of cells by killing over 90% of MDA-MB231 and over 80% of the CSCs in vitro. Virus used as therapy demonstrated that pathogenic microorganisms can be modify genetically and use for targeting diseases and oHSV can be used for treatment breast CSCs that are still not understood. This study demonstrates that oHVS effective against breast cancer stem cells and could be a beneficial method for treating cancer stem cells expressed in breast cancer.

Dheyab A.S.,Indian Academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2013

Infections caused by antibiotic-resistant bacteria result in higher mortality and antibiotic resistance are a recognized major public health threat. S. aureus is a gram-positive microorganism with numerous virulence factors having the ability to acquire antibiotic resistance from the environment. The pathogenic S. aureus were isolated from hospital samples and indentified on the basis of morphological and biochemical characteristics. The antibiotic resistance pattern of these samples was determined by disc diffusion method against 10 antibiotics (Amoxicillin, Gentamicin, Methionine, Cloxacillin, Fluconazol, Ampicillin, Rifampcin, Tetracycline, Azithromycin and Cefixime). Furthermore, DNA fingerprint was performed for these samples using Random primers. Then dendrogram analysis was done to determine the genomic similarity of these samples.

Mohsen A.M.A.,Indian academy Degree College
International Journal of Pharma and Bio Sciences | Year: 2013

India has one of the largest pools of hepatitis B-infected patients. The presence of HBs Ag in serum or plasma is an indication of active Hepatitis B infection either acute or chronic. Quantitative detection of Hepatitis B virus (HBV) DNA in serum by real time polymerase chain reaction (PCR) assay emerged as a gold standard in guided anti viral therapy. Evidences are suggestive of association of HBV genotype in liver dysfunction. We evaluated the performance as well as usefulness of both ELISA and PCR for detection of the major blood borne pathogen, HBV in southern India. First ALT and AST enzymes level was determined to check liver function. Serum of patients with chronic hepatitis B (HBsAg positive) and healthy individuals were tested both quantitatively and qualitatively. 4.5% of participants were HBsAg Positive by ELISA, while 7.5% of participants showed HBV DNA in real time PCR.

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