Huang F.,Fujian Academy of Agricultural science |
Huang F.,Incubator of National Key Laboratory of Crop Germplasm Enhancement and Molecular Breeding Between Fujian and the Ministry of science and Technology Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding of South China |
Huang F.,South China Base of National Key Laboratory of Hybrid Rice of China |
Huang F.,National Engineering Laboratory of Rice |
And 25 more authors.
Molecular Breeding | Year: 2014
Rice blast, caused by the fungus Magnaporthe oryzae, is one of the diseases most responsible for significantly decreasing the yield and quality of commercially grown rice. This study investigated the blast resistance response of japonica rice variety Yunyin (YY) treated with the Sichuan-43 isolate of M. oryzae. Seedlings of YY exhibited stronger resistance to blast than those of Lijiangxintuanheigu (LTH). The gene expression profile of YY treated with Sichuan-43 was determined using whole-genome microarray technology. Bioinformatics was used to identify putative resistance-related genes from the large number of genes assayed. Five candidate genes were further characterized by gene ontology classification analysis and pathway enrichment analysis, and were then integrated into various types of gene network regulation diagrams. Systematic bioinformatic analysis of the microarray provided a transcriptome map of YY, which helped to elucidate the mechanisms involved in blast resistance. Our results enhance the current understanding of the effects of rice blast resistance genes at the transcription level, and will facilitate further exploration of the molecular mechanism of blast resistance in YY. © 2014, Springer Science+Business Media Dordrecht.