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National Institute Of Agrobiological Science and Incorporated Administrative Agency Food And Agricultural Materials Inspection Center | Date: 2010-02-18

The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.


Saito R.,Incorporated Administrative Agency Food and Agricultural Materials Inspection Center | Ikenaga O.,Chiyoda Corporation | Ishihara S.,Incorporated Administrative Agency Food and Agricultural Materials Inspection Center | Shibata H.,Incorporated Administrative Agency Food and Agricultural Materials Inspection Center | And 3 more authors.
Journal of Pesticide Science | Year: 2010

In the present study, clopyralid residues in several crops grown in soils contaminated with clopyralid from compost made from animal manure were determined. Two field trials (cabbage and lettuce) and four pot trials (turnip, spinach, broccoli, and komatsuna) were conducted. An efficient extraction and cleanup procedure for analyzing clopyralid from crops followed by LC/MS/MS was developed. The residue levels of clopyralid in crops were from trace to 0.04 mg/kg, which were lower than the maximum residue limits (MRLs) permitted by the food sanitation law in Japan, with the exception of the komatsuna leaf trial. © Pesticide Science Society of Japan. Source


Ishihara S.,Incorporated Administrative Agency Food and Agricultural Materials Inspection Center | Toyodome N.,Incorporated Administrative Agency Food and Agricultural Materials Inspection Center
Journal of Pesticide Science | Year: 2012

To ensure the safety of the environment and human health, the manufacture of pesticide formulations must be in accord with registered formula components. The Agricultural Chemicals Inspection Station therefore regularly inspects the formula components of commercial samples. The purpose of this paper is to introduce a simple method using Fourier transform infrared attenuated total reflection (FT-IR/ATR) spectroscopy for the identification of the formula components of registered and commercial samples. The FT-IR/ATR technique is fast and relatively easy to use; it requires no sample pretreatment for most solid and liquid pesticide formulations, with the exception of some ready-touse liquid formulations. The transmittance spectrum (4,000-650 cm -1) is useful for inspection. We used three evaluation methods to identify the components of the formula: overlap of two spectra, calculation of the purity index, and use of principal component analysis (PCA). © Pesticide Science Society of Japan. Source

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