INCDMI Cantacuzino

Bucharest, Romania

INCDMI Cantacuzino

Bucharest, Romania
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Ion R.M.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Ion R.M.,University Valahia of Targoviste | Fierascu R.C.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Fierascu R.C.,University Valahia of Targoviste | And 3 more authors.
Science of Advanced Materials | Year: 2010

The unique shape of the fullerene C60 make them good candidates for preparation of supramolecular assemblies with different molecules, as porphyrins. The purpose of our study is the in vitro analysis of the triad tetraphenyl-porhyrin (TPP)-poly(vinylpyrrolidone) (PVP)-fullerene (C60)-(TPPPVP -C60). The cell viabilities before and after irradiations with TPP-PVP-C60 triad has been tested on K562 (leukemia cell line). The uptake of sensitizers into K562 (leukemia cell line) was studied using 1 microM of sensitizer and different times of incubation. In biological medium, no dark cytotoxicity was observed using sensitizer concentrations 0.05-50 microM and 1-18 h of incubation. The cell survival after irradiation of the cells with visible light was dependent upon light-exposure level. A higher photocytotoxic effect was observed for TPP-PVP-C60 triad, which inactivates 80% of cells after 15 min of irradiation. These results show that molecular triad, are a promising model for phototherapeutic agents, with potential applications in cell inactivation by PDT. © 2010 American Scientific Publishers.


Fierascu R.C.,University Valahia of Targoviste | Fierascu R.C.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Dumitriu I.,University Valahia of Targoviste | Dumitriu I.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | And 7 more authors.
Scientific Study and Research: Chemistry and Chemical Engineering, Biotechnology, Food Industry | Year: 2011

Fullerenic soluble derivatives are essential for many biomedical techniques that exploit the chemical and physical properties of these unique structural carbon nanospheres. Their toxicity, demonstrated in vitro and in vivo is important for the characterization and the limitation of these applications. The photo toxicity of some fullerene molecules has been identified as a future therapeutic tool. The specific objective of the study is the synthesis of C60 derivatives. Starting from the characteristics of fullerene compounds, we tried to study in vitro C60 fullerene and some functionalized derivatives (C60 complexes with poly-vinyl pyrrolidone (PVP) and with the oxo-dimer (Fe-O)2TPP), experimental models in vitro with normal and tumor cells and investigation of their toxicological profile, in order to identify novel anti-neoplasic therapeutic devices.


Fierascu R.C.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Fierascu R.C.,University Valahia of Targoviste | Dumitriu I.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Dumitriu I.,University Valahia of Targoviste | And 5 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2010

The nanoengineering of various materials for biomedical application has became in the last decade one of the most important research areas, due to the continuous struggle to find new and more efficient instruments for the therapy of cancer and other diseases. Attempts to obtain functionalized derivatives of fullerene seek the synthesis of water-soluble materials, in order to investigate their effects in physiological conditions. International studies on the biological properties of fullerenes and their derivatives, are now targeted towards anti-tumor effects, pharmacology and their involvement in oxidative stress. Their toxicity, demonstrated both in vitro and in vivo is important for characterization and selection of applications. Phototoxicity of some molecules of fullerenes has been identified as future therapeutic tool. The present paper describes the synthesis, characterization and some biomedical applications of some nanomaterials based on fullerenes. © 2010 SPIE.


Valenciano M.,EpiConcept | Kissling E.,EpiConcept | Reuss A.,Robert Koch Institute | Jimenez-Jorge S.,CIBER ISCIII | And 25 more authors.
Vaccine | Year: 2015

Background: In the first five I-MOVE (Influenza Monitoring Vaccine Effectiveness in Europe) influenza seasons vaccine effectiveness (VE) results were relatively homogenous among participating study sites. In 2013-2014, we undertook a multicentre case-control study based on sentinel practitioner surveillance networks in six European Union (EU) countries to measure 2013-2014 influenza VE against medically-attended influenza-like illness (ILI) laboratory-confirmed as influenza. Influenza A(H3N2) and A(H1N1)pdm09 viruses co-circulated during the season. Methods: Practitioners systematically selected ILI patients to swab within eight days of symptom onset.We compared cases (ILI positive to influenza A(H3N2) or A(H1N1)pdm09) to influenza negative patients. We calculated VE for the two influenza A subtypes and adjusted for potential confounders. We calculated heterogeneity between sites using the I2 index and Cochrane's Q test. If the I2 was <50%, we estimated pooled VE as (1 minus the OR)×100 using a one-stage model with study site as a fixed effect. If the I2 was >49% we used a two-stage random effects model. Results: We included in the A(H1N1)pdm09 analysis 531 cases and 1712 controls and in the A(H3N2) analysis 623 cases and 1920 controls. For A(H1N1)pdm09, the Q test (p=0.695) and the I2 index (0%) suggested no heterogeneity of adjusted VE between study sites. Using a one-stage model, the overall pooled adjusted VE against influenza A(H1N1)pdm2009 was 47.5% (95% CI: 16.4-67.0).For A(H3N2), the I2 was 51.5% (p=0.067). Using a two-stage model for the pooled analysis, the adjusted VE against A(H3N2) was 29.7 (95% CI: -34.4-63.2). Conclusions: The results suggest a moderate 2013-2014 influenza VE against A(H1N1)pdm09 and a low VE against A(H3N2). The A(H3N2) estimates were heterogeneous among study sites. Larger sample sizes by study site are needed to prevent statistical heterogeneity, decrease variability and allow for two-stage pooled VE for all subgroup analyses. © 2015 The Authors.


Trichophyton rubrum is the most common dermatophyte isolated from human dermatophytosis. We present the mycological diagnosis algorithm and results in an onychomycosis case--tinea unguium. Biological samples have been inoculated on both usual and special culture media and incubated at both 30 degrees C and 37 degrees C for a period of fourteen days. Based on the macroscopic and microscopic characters and on the physiological and biochemical tests results, we identified a Trichophyton rubrum granular type dermatophyte. We are discussing phenotypic mycological diagnosis limits and molecular diagnostic benefits for rapid setting up of fungal therapy in order to avoid chronic mycosis and their complications.


Marinescu B.,Victor Babes | Coman C.,INCDMI Cantacuzino
Revista Romana de Bioetica | Year: 2010

The progress of medical science could not have been developed to the actual stage without involving laboratory animals. The research in this field depends sometime on the work with laboratory animals. It is impossible to do research using laboratory animals and not to create a minimum discomfort to them. The necessity of reducing the animals distress appeared at the same time with the necessity of their use, but today this is a major issue, and the proof is the big amount of money available to the studies on the improvement this process. Researchers make studies to find the methods to replace the in vivo techniques or to improve them, in order to produce less suffering on animals. At the same time of these studies, a new institution has been established, with the aim to evaluate and certify the experiments. This aim may be achieved by dividing the animal testing into the following three stages: pre-experimental, experimental and post-experimental. The goal of all these efforts is to replace the use of animals, to reduce their use in experiments and, when they are still used, to be treated as human as possible.


Enterococci are a frequent cause of a variety of human infections, the most common being urinary tract infection followed by bacteraemia, meningitis and endocarditis from hospitalized patients. The study and monitoring of antibiotic resistance of enterococci strains isolated and confirmed in 2009. Identification of 30 strains received in 2009 was based on phenotypic characteristics (microscopy, culture and biochemical characters); antibiotic susceptibility testing was performed according to CLSI standards 2009 by diffusion test and MIC by agar dilution and E-test. The strains belonged to the following species: E. faecalis (18), E. faecium (6), E. gallinarum (5), and E. durans (1), and were isolated from: blood cultures, urine, pus-wound, cerebrospinal fluid (CSF) and catheter. Antibiotic susceptibility testing showed susceptibility to different antibiotics depending on the species and also on the type of clinical samples. Due to the small number of strains, no resistance phenotypes could be determined. As emergence of antibiotic resistant enterococci is well known, continuous surveillance of antibiotic susceptibility and molecular study of this issue are required.


PubMed | INCDMI Cantacuzino.
Type: Journal Article | Journal: Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990) | Year: 2011

Enterococci are a frequent cause of a variety of human infections, the most common being urinary tract infection followed by bacteraemia, meningitis and endocarditis from hospitalized patients.The study and monitoring of antibiotic resistance of enterococci strains isolated and confirmed in 2009.Identification of 30 strains received in 2009 was based on phenotypic characteristics (microscopy, culture and biochemical characters); antibiotic susceptibility testing was performed according to CLSI standards 2009 by diffusion test and MIC by agar dilution and E-test.The strains belonged to the following species: E. faecalis (18), E. faecium (6), E. gallinarum (5), and E. durans (1), and were isolated from: blood cultures, urine, pus-wound, cerebrospinal fluid (CSF) and catheter. Antibiotic susceptibility testing showed susceptibility to different antibiotics depending on the species and also on the type of clinical samples.Due to the small number of strains, no resistance phenotypes could be determined. As emergence of antibiotic resistant enterococci is well known, continuous surveillance of antibiotic susceptibility and molecular study of this issue are required.


PubMed | INCDMI Cantacuzino.
Type: Journal Article | Journal: Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990) | Year: 2010

Trichophyton rubrum is the most common dermatophyte isolated from human dermatophytosis. We present the mycological diagnosis algorithm and results in an onychomycosis case--tinea unguium. Biological samples have been inoculated on both usual and special culture media and incubated at both 30 degrees C and 37 degrees C for a period of fourteen days. Based on the macroscopic and microscopic characters and on the physiological and biochemical tests results, we identified a Trichophyton rubrum granular type dermatophyte. We are discussing phenotypic mycological diagnosis limits and molecular diagnostic benefits for rapid setting up of fungal therapy in order to avoid chronic mycosis and their complications.

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