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Rome, Italy

Avino P.,INAIL Research Area | Capannesi G.,ENEA | Renzi L.,ENEA | Rosada A.,ENEA
Journal of Radioanalytical and Nuclear Chemistry | Year: 2015

Hair element characterization of homogeneous population (no-smoker teenagers, 15–19 years, 27 males, 104 females) along with the relationship between elements (32 analyzed by INAA) and some physiological parameters is reported: the inorganic fraction content varies according to sex, hair natural color and hair type. Correlations between elements allowed to extrapolate interesting considerations. Hair color shows slightly positive correlation values for S, Zn, Ba and Mo whereas hair type does not show significant correlations among various elements. Finally, a canonical analysis showed high positive coefficients for some alkaline-earth elements and other of exogenous origin. © 2015, Akadémiai Kiadó, Budapest, Hungary.

Russo M.V.,University of Molise | Avino P.,INAIL Research Area | Perugini L.,University of Molise | Notardonato I.,University of Molise
RSC Advances | Year: 2016

An Ultrasound-Vortex-Assisted Dispersive Liquid-Liquid Micro-Extraction (USVADLLME) procedure coupled with Gas Chromatography-Ion Trap Mass Spectrometry (GC-IT/MS) is proposed for the fast analysis of nine polycyclic aromatic hydrocarbons (fluorene, phenanthrene, anthracene, fluoranthene, pyrene, chrysene, benzo(b)fluoranthene, benzo(a)pyrene and benzoperylene) in beer (alcohol by volume ≤7%). Among 5 possible extraction solvents tested, dichloromethane, density 1.325 g mL-1 at 25 °C, was selected for method development. Parameters such as extraction solvent type and volume, extraction time and pH, and NaCl concentration were optimized. Under optimal conditions, the enrichment factors of the nine analytes range between 100 and 200 fold, the recoveries from 83% to 99% and the correlation coefficients from 0.9982 to 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) are ≥3.8 pg μL-1 and ≥9.8 pg μL-1, respectively. The precision expressed as relative standard deviation (RSD), is ≤4.0%. The whole proposed methodology was demonstrated to be simple, reproducible and sensitive for the determination of trace PAHs in beer samples. © The Royal Society of Chemistry 2016.

Andreoli R.,Laboratory of Industrial Toxicology | Andreoli R.,University of Parma | Protano R.,University of Rome La Sapienza | Manini P.,Laboratory of Industrial Toxicology | And 7 more authors.
Medicina del Lavoro | Year: 2012

Objectives: To evaluate the association between environmental exposure to benzene and oxidative damage to nucleic acids in children, also considering the role of Environmental Tobacco Smoke (ETS). Methods: 396 children living in central Italy were recruited in districts with different urbanization and air pollution. All biomarkers were determined in spot urine samples by mass spectrometric techniques to assess exposure [benzene (U-Benz), and its metabolites (t,t-muconic and S-phenylmercapturic acids, t,t-MA and S-PMA, respectively), cotinine] and nucleic acid oxidation [8-oxo-7,8-dihydro-2'- deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-oxo-7,8-dihydroguanine (8-oxoGua)]. Results: Biomarkers of exposure and nucleic acid oxidation increased with urbanization and were correlated with each other (r>0.18, p<0.005). In a multiple linear regression model, benzene exposure, assessed by S-PMA and t,t-MA, was associated (p<0.0001) with both 8-oxodGuo (R2=0.392) and 8-oxoGuo (R2=0.193) in all areas of residence, with similar slopes. Conclusions: (i) Biomarkers of exposure to benzene increased as a function of environmental air pollution and urbanization level; (ii) U-Benz clearly distinguished both exposure to ETS and areas of residence, whereas benzene metabolites were associated only with the latter; (iii) the variance of 8-oxodGuo and 8-oxoGuo was accounted for by environmental benzene exposure, thus suggesting that benzene is a good tracer of other components of complex mixtures of pollutants causing oxidative damage to nucleic acids.

Avino P.,INAIL Research Area | Manigrasso M.,INAIL Research Area
Environmental Science and Pollution Research | Year: 2016

Many studies show that particle toxicity increases with decreasing their size, emphasizing the role of submicrometric particles, in particular of ultrafine particles (<100 nm). In fact, particles greater than 2.5 μm are quickly removed through dry and wet deposition on the timescale of hours whereas submicrometer particles may reside in atmosphere for weeks, penetrate in indoor environment, and be long-range transported. High aerosol size resolution measurements are important for a correct assessment of the deposition efficiency in the human respiratory system, and time resolution is another important requisite. Starting from such considerations, time-resolved aerosol particle number size distributions have been measured in downtown Rome. Fast Mobility Particle Sizer (FMPS) and Scanning Mobility Particle Sizer (SMPS) measurements have been carried out at the INAIL’s Pilot Station, located in downtown Rome, in an area characterized by high density of autovehicular traffic. The two instruments have allowed to investigate deeply the urban aerosol in the range of 5.6–560 and 3.5–117 nm, respectively. In particular, the FMPS measurements have confirmed the interpretation about the transition phenomena in the time interval of few seconds, timescale typically associated with the emission of gasoline and diesel engines. In downtown Rome, the hourly average size distribution is bimodal or trimodal with maxima at about 5–15, 20–30, and 70–100 nm. Particle formation in the nucleation mode was associated to freshly emitted autovehicular exhaust. © 2016 Springer-Verlag Berlin Heidelberg

Russo M.V.,University of Molise | Avino P.,INAIL Research Area
Natural Product Research | Year: 2016

The study and analysis of the materials employed in artistic paintings provide deeper knowledge about the history of the work of art, including restoration efforts made in the past, and the development of painting techniques through the centuries. Gas chromatography coupled to mass spectrometry is the main analytical technique employed, as it proved to be the most suitable technique for the analysis of complex mixtures, thanks to its combination of sensitivity, wide range of applicability and versatility. Further, μFT-IR technique has also been employed to get a preliminary screening of the samples taken from paintings. In this paper, the analytical protocol based on these two techniques has been applied for analysing natural terpenic resins; its performance has been tested on microsamples collected from paintings of valuable artistic interest. © 2016 Taylor & Francis

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