Mhadhbi H.,British Petroleum |
Fotopoulos V.,Cyprus University of Technology |
Mylona P.V.,Greek National Agricultural Research Foundation |
Jebara M.,British Petroleum |
And 3 more authors.
Physiologia Plantarum | Year: 2011
Antioxidant responses and nodule function of Medicago truncatula genotypes differing in salt tolerance were studied. Salinity effects on nodules were analysed on key nitrogen fixation proteins such as nitrogenase and leghaemoglobin as well as estimating lipid peroxidation levels, and were found more dramatic in the salt-sensitive genotype. Antioxidant enzyme assays for catalase (CAT, EC 220.127.116.11), superoxide dismutase (EC 18.104.22.168), ascorbate peroxidase (EC 22.214.171.124) and guaiacol peroxidase (EC 126.96.36.199) were analysed in nodules, roots and leaves treated with increasing concentrations of NaCl for 24 and 48 h. Symbiosis tolerance level, depending essentially on plant genotype, was closely correlated with differences of enzyme activities, which increased in response to salt stress in nodules (except CAT) and roots, whereas a complex pattern was observed in leaves. Gene expression responses were generally correlated with enzymatic activities in 24-h treated roots in all genotypes. This correlation was lost after 48 h of treatment for the sensitive and the reference genotypes, but it remained positively significant for the tolerant one that manifested a high induction for all tested genes after 48 h of treatment. Indeed, tolerance behaviour could be related to the induction of antioxidant genes in plant roots, leading to more efficient enzyme stimulation and protection. High induction of CAT gene was also distinct in roots of the tolerant genotype and merits further consideration. Thus, part of the salinity tolerance in M. truncatula is related to induction and sustained expression of highly regulated antioxidant mechanisms. © Physiologia Plantarum 2010. Source
Ina | Date: 2010-09-14
Computer programs for protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs by computing and comparing the prints of audiovisual programs; computer software for protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs by computing and comparing the prints of audiovisual programs; software packages for protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs by computing and comparing the prints of audiovisual programs; data banks, namely, electronic databanks in the field of for protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs recorded on computer media; electronic data bases, namely, electronic databases in the field of protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs recorded on computer media; audiovisual, radio and photographic archive banks, namely, electronic databases in the field of audio, video and photographs recorded on computer media; magnetic recording media, namely, blank magnetic tapes for tapes recorder; blank video cassettes, blank audio cassettes, blank digital video discs, blank digital audio discs, blank floppy discs; sleeves for discs and audio cassettes. Advertising; business management; business administration; business administration and management, namely, administrative management in control of audiovisual, sound and photo programmes; dissemination, distribution and publication of advertisements; billboard advertising; compilation of documentary data on audiovisual, sound and photo programmes; opinion surveys, polls, market studies; assistance in management of business activities, namely, assistance to industrial or commercial enterprises in managing and organizing their affairs; public relations, organizing exhibitions for commercial or advertising purposes; management of computer files and databases relating to the audiovisual field; subscriptions to information media, namely, television channels, electronic journals; exhibition site management, namely, arranging and conducting of fairs and exhibitions for business and advertising purposes. Restoration and maintenance of sound and audiovisual archive works. Communication services and news and information agencies, namely, the transmission of news items to news reporting organizations; dissemination of information, communication of radio programmes or television using any means, namely, radio and television programme broadcasting; telephone communications; data communication services or remote computing services for transmitting information contained in a data bank or database, namely, data communication by electronic mail; communication and telecommunication media via computer terminals for transmission of information held in a database or a data bank, particularly over all computer networks of the Internet type, namely, communications by electronic computer terminals, communications by telephone; correspondence exchange, namely, electronic mail. Publishing and lending, of books, reviews, journal and periodicals; electronic publishing of books and periodicals in audiovisual, sound and photo programmes by any means and on all types of media whether analogue or digital, namely, electronic publishing services, namely, publication of texts and graphic works of others on CD, DVD, on-line featuring audiovisual programs; editing of audiovisual and sound programmes, namely, editing of radio and television programmes; organizing and holding colloquiums, conferences, conventions and seminars and events for cultural or educational purposes; organizing competitions of an educational and entertainment nature, namely, sports competitions; operating of lotteries; videotape filming, namely, videotape film production; photo-reporting. Consulting, assistance, studies and research in the field of protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs; scientific and technical consulting, information in the audiovisual field; computer programming; designs, development and updating of software; creation of databases and data banks, namely, database development services; hosting computer sites; scientific and industrial research in the field of protection, digitization, preservation, identification, monetization, description, documentation, valorisation, analysis, filtering, scanning and monitoring of audiovisual programs. Legal services, namely, management of intellectual property rights in connection with audiovisual and sound programs; legal services.
'Once fully completed, our new tool will be made available to conservation scientists from around the world at an affordable cost (an assay can cost around EUR 0.5 per target), which will facilitate greater knowledge about historical works of art and help international museums, restoration art studios and laboratories to plan the best conservation and preventive strategies,' explains NANOART project coordinator Dr Jesus de la Fuente from the CSIC/University of Zaragoza, Spain. In addition, the sensitiveness of the project's new nanotechnology-based methods means that smaller samples are required to be taken from the artwork for analysis. This in itself will help to better preserve our cultural heritage. In order to characterise ancient paints, experts have often relied on conventional molecular biology methodologies that were developed decades ago. The concept behind the NANOART project was that these techniques could be substituted by more sensitive, inexpensive and faster techniques that take advantage of emerging nanotechnologies. Furthermore, conventional methods – apart from being expensive – are also only available at a few laboratories, and require specialised personnel and equipment. A key objective of the NANOART project has been to address the cost issue by applying techniques developed for clinical diagnosis. In this way, the project is also highly original as it aims to take latest developments in clinical medicine and apply them to the conservation and preservation of cultural heritage. 'The innovative nature of the project is also denoted by the fact that there is currently no method or kit available that can be easily used at point-of-care to analyse paints without requiring expensive equipment and extensive training,' says Ana Claro, research fellow from the INA/University of Zaragoza. 'With the NANOART kit, the final user will be able to conduct an affordable analysis (in some cases at the cost of only a few euros) by simply following the instructions. Within a four-hour period, the results will be available.' The potential opportunities opened up by the new analytical nanotechnology are huge. For example, developed in parallel with the NANOART kit, a spin-off company called NanoImmunotech has been launched in order to develop devices to detect bacterial infection in meat using the same technology as used in NANOART. 'This opens our technology to other applications far from cultural heritage applications,' says de la Fuente. 'However, we would like to continue further developing novel uses of NANOART technology for other applications in cultural heritage, and our next step will be to look for funding to develop an even more user friendly device.' Explore further: Should we 3D print a new Palmyra? More information: For further information please visit the project coordinator website: www.unizar.es/EN
Mhadhbi H.,Laboratory of Legumes LL |
Fotopoulos V.,INA |
Fotopoulos V.,Cyprus University of Technology |
Mylona P.V.,Greek National Agricultural Research Foundation |
And 4 more authors.
Journal of Plant Physiology | Year: 2013
Alternative oxidase (AOX) is the central component of the non-phosphorylating alternative respiratory pathway in plants and may be important for mitochondrial function during environmental stresses. Recently it has been proposed that Aox can be used as a functional marker for breeding stress tolerant plant varieties. This requires characterization of Aox alleles in plants with different degree of tolerance in a certain stress, affecting plant phenotype in a recognizable way. In this study we examined Aox1 gene expression levels in Medicago truncatula genotypes differing in salt stress tolerance, in order to uncover any correlation between Aox expression and tolerance to salt stress. Results demonstrated a specific induction of Aox1 gene expression in roots of the tolerant genotype that presented the lowest modulation in phenotypic and biochemical stress indices such as morphologic changes, protein level, lipid peroxidation and ROS generation. Similarly, in a previous study we reported that induction of antioxidant gene expression in the tolerant genotype contributed to the support of the antioxidant cellular machinery and stress tolerance. Correlation between expression patterns of the two groups of genes was revealed mainly in 48. h treated roots. Taken together, results from both experiments suggest that M. truncatula tolerance to salt stress may in part due to an efficient control of oxidative balance thanks to (i) induction of antioxidant systems and (ii) involvement of the AOX pathway. This reinforces the conclusion that differences in antioxidant mechanisms can be essential for salt stress tolerance in M. truncatula and possibly the corresponding genes, especially Aox, could be utilized as functional marker. © 2012 Elsevier GmbH. Source
Joly A.,French Institute for Research in Computer Science and Automation |
Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition | Year: 2011
Following the success of hashing methods for multidimensional indexing, more and more works are interested in embedding visual feature space in compact hash codes. Such approaches are not an alternative to using index structures but a complementary way to reduce both the memory usage and the distance computation cost. Several data dependent hash functions have notably been proposed to closely fit data distribution and provide better selectivity than usual random projections such as LSH. However, improvements occur only for relatively small hash code sizes up to 64 or 128 bits. As discussed in the paper, this is mainly due to the lack of independence between the produced hash functions. We introduce a new hash function family that attempts to solve this issue in any kernel space. Rather than boosting the collision probability of close points, our method focus on data scattering. By training purely random splits of the data, regardless the closeness of the training samples, it is indeed possible to generate consistently more independent hash functions. On the other side, the use of large margin classifiers allows to maintain good generalization performances. Experiments show that our new Random Maximum Margin Hashing scheme (RMMH) outperforms four state-of-the-art hashing methods, notably in kernel spaces. © 2011 IEEE. Source