In Vitro Brazil Ltda Research Division

Mogi Mirim, Brazil

In Vitro Brazil Ltda Research Division

Mogi Mirim, Brazil

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Sanches B.V.,In Vitro Brazil Ltda Research Division | Sanches B.V.,State University Londrina | Pontes J.H.F.,In Vitro Brazil Ltda Research Division | Pontes J.H.F.,State University Londrina | And 4 more authors.
Reproduction in Domestic Animals | Year: 2013

Contents: In this work, we evaluated whether embryo development and pregnancy rates would be affected by culturing bovine Bos indicus embryos in Synthetic Oviductal Fluid with amino acids (SOFaa) or G1/G2 sequential medium under a low-oxygen atmosphere. Using Ovum Pick Up, we obtained 1,538 oocytes, divided into G1/G2 (n=783) and SOFaa (n=755). No difference was observed for blastocyst development among the groups (27.8%±14.6 and 34.9%±20.0 for G1/G2 and SOFaa respectively, p>0.05). Transferring the embryos (n=450) from both groups to recipients resulted in similar pregnancy rates for the G1/G2 (38.4% n=78/203) compared to the SOFaa (39.7% n=98/247). Our findings confirm that Bos indicus embryos cultured in SOFaa and G1/G2 under low-oxygen atmosphere have similar in vitro (blastocyst rate) and in vivo (pregnancy rate) developmental capacity. However, embryos cultured in G1/G2 medium have higher cleavage than those cultured in SOFaa medium. © 2012 Blackwell Verlag GmbH.


PubMed | In Vitro Brazil Ltda Research Division
Type: Journal Article | Journal: Reproduction in domestic animals = Zuchthygiene | Year: 2013

In this work, we evaluated whether embryo development and pregnancy rates would be affected by culturing bovine Bos indicus embryos in Synthetic Oviductal Fluid with amino acids (SOFaa) or G1/G2 sequential medium under a low-oxygen atmosphere. Using Ovum Pick Up, we obtained 1,538 oocytes, divided into G1/G2 (n = 783) and SOFaa (n = 755). No difference was observed for blastocyst development among the groups (27.8% 14.6 and 34.9% 20.0 for G1/G2 and SOFaa respectively, p > 0.05). Transferring the embryos (n = 450) from both groups to recipients resulted in similar pregnancy rates for the G1/G2 (38.4% n = 78/203) compared to the SOFaa (39.7% n = 98/247). Our findings confirm that Bos indicus embryos cultured in SOFaa and G1/G2 under low-oxygen atmosphere have similar in vitro (blastocyst rate) and in vivo (pregnancy rate) developmental capacity. However, embryos cultured in G1/G2 medium have higher cleavage than those cultured in SOFaa medium.

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