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Sanches B.V.,In Vitro Brazil Ltda Research Division | Sanches B.V.,State University Londrina | Pontes J.H.F.,In Vitro Brazil Ltda Research Division | Pontes J.H.F.,State University Londrina | And 4 more authors.
Reproduction in Domestic Animals | Year: 2013

Contents: In this work, we evaluated whether embryo development and pregnancy rates would be affected by culturing bovine Bos indicus embryos in Synthetic Oviductal Fluid with amino acids (SOFaa) or G1/G2 sequential medium under a low-oxygen atmosphere. Using Ovum Pick Up, we obtained 1,538 oocytes, divided into G1/G2 (n=783) and SOFaa (n=755). No difference was observed for blastocyst development among the groups (27.8%±14.6 and 34.9%±20.0 for G1/G2 and SOFaa respectively, p>0.05). Transferring the embryos (n=450) from both groups to recipients resulted in similar pregnancy rates for the G1/G2 (38.4% n=78/203) compared to the SOFaa (39.7% n=98/247). Our findings confirm that Bos indicus embryos cultured in SOFaa and G1/G2 under low-oxygen atmosphere have similar in vitro (blastocyst rate) and in vivo (pregnancy rate) developmental capacity. However, embryos cultured in G1/G2 medium have higher cleavage than those cultured in SOFaa medium. © 2012 Blackwell Verlag GmbH.

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