Imphal College

Imphal, India

Imphal College

Imphal, India
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Bag G.C.,Imphal College | Grihanjali Devi P.,Imphal College | Bhaigyabati T.,Institutional Biotech Hub
International Journal of Pharmaceutical Sciences Review and Research | Year: 2016

Aqueous and methanol extracts of Meyna laxiflora leaf, fruit pulp and seed were screened for the presence of phytochemical constituents. Total phenolic content, total flavonoid content and antioxidant activity in the extracts were also determined. Phytochemicals were extracted using soxhlet extraction method. Phytochemical screening showed the presence of most of the phytoconstituents in aqueous and methanol extracts of various parts of M. laxiflora. Methanol was found to be a better solvent for extraction of phytochemicals from M. laxiflora. Total phenolic content is expressed in terms of gallic acid equivalent (GAE) and was found to be highest in methanolic fruit pulp extract (129.54 ± 0.25mg /g of extract). Total flavonoid content is expressed in terms of quercetin equivalent (QE) and methanolic leaf extract recorded the highest (80.45 μg ± 0.16 /100g of dried extract) followed by fruit pulp and seed extracts. Results indicate that aqueous and methanol extracts of various parts of M. laxiflora were found to possess DPPH scavenging activity, ferric reducing power and total antioxidant activity in dose dependent manner. In the entire antioxidant assay performed methanol extracts were found to have higher activity than aqueous extracts and among the various parts of M. laxiflora used for the study, fruit pulp was showing higher activity than leaf and seed which may be due to higher total phenolic content. © 2016, Global Research Online. All rights reserved.


Lemino Singh K.,Institutional Biotech Hub | Bag G.C.,Imphal College
International Journal of PharmTech Research | Year: 2013

Hedychium is a rhizomatous plant belonging to family Zingiberaceae. In this present study, three different species of genus Hedychium i.e. H. spicatum, H. coronarium and H. rubrum were analysed quantitatively for the presence of major phytochemical compounds. The total phenolic contents in extracts of the three different species were also analyzed. The dried and powdered rhizomes of each species were extracted with distilled water using Soxhlet extraction method. Phytochemical screening of the water extract of H. spicatum showed the presence of phenolic compounds, flavonoids, reducing sugar (carbohydrate), protein, steroids and triterpenoids, cardiac glycosides, tannin, saponin and oil. Phytochemical screening of the water extracts of both H. coronarium and H. rubrum also showed the presence of similar groups of compounds. H. spicatum, H. coronarium and H. rubrum tested negative for the presence of alkaloids. Both H. spicatum and H. coronarium showed negative test for phlobatannin but H.rubrum indicated the presence of phlobatannin. The total phenolic contents of the water extracts of each species were estimated by using UV Visible Spectrophotometer and expressed as gallic acid equivalents (GAE). The total phenolic contents of the water extracts of H. spicatum, H. coronarium and H. rubrum in terms of gallic acid equivalent were 29.39 ± 0.01, 34.93 ± 0.01 and 66.48 ± 0.01 mg /g of extract powder respectively.


Lemino Singh K.,Institutional Biotech Hub | Ranjit Singh L.,Imphal College | Grihanjali Devi P.,Imphal College | Romabati Devi N.,Imphal College | And 2 more authors.
International Journal of PharmTech Research | Year: 2013

Hedychium is a rhizomatous perennial plant which belongs to family Zingiberaceae with various ethnomedicinal and ornamental significance. In this study, three different species of genus Hedychium i.e. H. spicatum, H. coronarium and H. rubrum were screened for the presence of major phytochemical compounds. The total phenolic contents of the three different species were also analyzed. The dried and powdered rhizomes of each species were extracted with methanol using Soxhlet extraction method. Phytochemical screening of methanolic extracts of each species was performed qualitatively. Phytochemical screening of the methanolic extract of H. spicatum showed the presence of phenolic compounds, flavonoids, alkaloids, reducing sugar (carbohydrate), protein, steroids and triterpenoids, cardiac glycosides, diterpene, tannin, saponin and oil. Phytochemical screening of the methanolic extracts of both H. coronarium and H. rubrum also showed the presence of similar groups of compounds but tested negative for the presence of alkaloids. Both H. spicatum and H. rubrum showed negative test for phlobatannin but H. coronarium indicated the presence of phlobatannin. The total phenolic contents of the methanolic extracts of each species were estimated by using UV Visible Spectrophotometer and expressed as gallic acid equivalents (GAE). The total phenolic contents of the methanolic extracts of H. spicatum, H. coronarium and H. rubrum in terms of gallic acid equivalent were 19.48±0.82, 26.22±1.17 and 28.16±0.45 mg /g of extract powder respectively.


Bag G.C.,Imphal College | Grihanjali Devi P.,Imphal College | Bhaigyaba T.,Institutional Biotech Hub
International Journal of Pharmaceutical Sciences Review and Research | Year: 2015

In the present study methanolic rhizome extract of three Hedychium species namely Hedychium spicatum, Hedychium coronarium and Hedychium rubrum were evaluated for antioxidant activity using free radical scavenging activity, reducing power and total antioxidant activity. Total flavonoid content was also determined in each extract. Ascorbic acid and quercetin were used as reference standards. Total flavonoid content in methanolic rhizome extract of H. spicatum, H. coronarium and H. rubrum in terms of quercetin equivalent (QE) were 4.22, 2.47 and 21.25μg/100g of dried rhizome respectively. In DPPH assay H. rubrum (60.86%) showed the highest free radical scavenging activity followed by H. Spicatum (42.74%) and lowest activity in H. coronarium (32.42%). In reducing power method, standard ascorbic acid and sample extracts were found to increase with increase in concentration. Total antioxidant activity of methanolic rhizome extract of H. spicatum, H. coronarium and H. rubrum in terms of ascorbic acid equivalent (AAE) was 224.4, 205.6 and 279μg/ml of extract respectively. Results indicate that methanolic rhizome extract of all the three Hedychium species are potential source of antioxidant which may be due to the presence of flavonoid in the extracts. H. rubrum showed the highest total flavonoid content and antioxidant activity among the three Hedychium species. © 2015, Global Research Online. All rights reserved.


Agrawal H.,Banasthali University | Singh L.I.,Imphal College | Saraswat P.K.,P.A. College | Saraswat V.K.,Banasthali University
AIP Conference Proceedings | Year: 2013

In the present paper, I-V characteristics and DC volume conductivity of Se-In-Sb semi-conducting glasses has been discussed. This is an effort towards the understanding of physics of temperature dependence of dc electrical volume conductivity of Se90In10-xSbx(X=2,4,6&8) glass in bulk form. These Chalcogenide glasses were prepared by melt quenching (rapid cooling of melt) technique. The amorphous nature of as prepared glass was confirmed by XRD. Using Keithley Electrometer / High resistance Meter 6517 A, the I-V characteristics of these glasses have been recorded in a temperature range from room to 100°C and the voltage range was taken from 10V to 150V for elevated temperatures. Variation in conductivity, derived from I-V curves, as a function of composition of samples is explained on the basis of bonds formed in the system. Additionally, the Poole-Frenkel conduction mechanism has also been verified. © 2013 AIP Publishing LLC.


Lahiri S.,Saha Institute of Nuclear Physics | Takao T.,Osaka University | Devi P.G.,Saha Institute of Nuclear Physics | Devi P.G.,Imphal College | And 6 more authors.
BioMetals | Year: 2012

Here we have examined the association of an aureolic acid antibiotic, chromomycin A3 (CHR), with Cu 2+. CHR forms a high affinity 2:1 (CHR:Cu 2+) complex with dissociation constant of 0.08 × 10 -10 M 2 at 25°C, pH 8.0. The affinity of CHR for Cu 2+ is higher than those for Mg 2+ and Zn 2+ reported earlier from our laboratory. CHR binds preferentially to Cu 2+ in presence of equimolar amount of Zn 2+. Complex formation between CHR and Cu 2+ is an entropy driven endothermic process. Difference between calorimetric and van't Hoff enthalpies indicate the presence of multiple equilibria, supported from biphasic nature of the kinetics of association. Circular dichroism spectroscopy show that [(CHR) 2:Cu 2+] complex assumes a structure different from either of the Mg 2+ and Zn 2+ complex reported earlier. Both [(CHR) 2:Mg 2+] and [(CHR) 2:Zn 2+] complexes are known to bind DNA. In contrast, [(CHR) 2:Cu 2+] complex does not interact with double helical DNA, verified by means of Isothermal Titration Calorimetry of its association with calf thymus DNA and the double stranded decamer (5′-CCGGCGCCGG-3′). In order to interact with double helical DNA, the (antibiotic) 2 : metal (Mg 2+ and Zn 2+) complexes require a isohelical conformation. Nuclear Magnetic Resonance spectroscopy shows that the Cu 2+ complex adopts a distorted octahedral structure, which cannot assume the required conformation to bind to the DNA. This report demonstrates the negative effect of a bivalent metal upon the DNA binding property of CHR, which otherwise binds to DNA in presence of metals like Mg 2+ and Zn 2+. The results also indicate that CHR has a potential for chelation therapy in Cu 2+ accumulation diseases. However cytotoxicity of the antibiotic might restrict the use. © Springer Science+Business Media, LLC. 2011.


Bhaigyabati T.,Institutional Biotech Hub | Devi P.G.,Imphal College | Bag G.C.,Imphal College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

Hedychium species is one of the important aromatic, ornamental medicinal plants of North east India. In the present study, total flavonoid content and antioxidant activity of aqueous rhizome extract of three Hedychium species namely H. rubrum, H. coronarium and H. spicatum were investigated. In antioxidant activity: free radical scavenging activity, reducing power and total antioxidant activity were determined and expressed in terms of standard used for the respective assay. Total flavonoid content of aqueous rhizome extract of H. rubrum, H. coronarium and H. spicatum in terms of quercetin equivalent (QE) was 3.22, 2.77 and 0.74 μg/100g of extract respectively. In DPPH assay H. rubrum (32.3%) showed the highest free radical scavenging activity followed by H. coronarium (21%) and lowest activity in H. Spicatum (5.76%). In reducing power method, both standard ascorbic acid and sample extracts were found to be increased in a dose dependent manner. Total antioxidant activity of aqueous rhizome extract of H. rubrum, H. coronarium and H. spicatum in terms of ascorbic acid equivalent (AAE) was 207.3, 157.5 and 102.6 μg/ml of extract. The present data indicates that aqueous rhizome extract of all the three Hedychium species are potential source of antioxidant which may be due to the presence of flavonoid in the extracts. Among the three Hedychium species, H. rubrum was found to have the highest total flavonoid content and antioxidant activity.


Hemanta Singh R.K.,Imphal College | Mohon Singh K.H.,Imphal College
International Journal of Earth Sciences and Engineering | Year: 2011

The rock exposures in the Tengnoupal-Sita road section comprise mainly of weak rock of Disang and Barail Groups characterized by shale with frequent intercalation of fine sandstone and siltstone beds, which is a prominent feature in the turbidite dominated flysch sediments of Tertiary in the state of Manipur. Besides, the rock mass is highly fractured and jointed because of intense folding and faulting associated with the India and Burma plate collision tectonics. Therefore, the rocks are highly weathered to such a degree that most of the rocks exposed all along the section may be treated as scientifically soil from engineering point of view. This weak nature of the rock in conjunction with the moderate to steep slope, thick mantle of waste and heavy rainfall, improper drainage, nonsystematic and excessive landuse render the rocks very vulnerable to instability and masswasting processes. The landslide study along the Tengnoupal-Sita road study has been based on the kinematic analysis of fractures and slope mass rating (SMR). From the study, it has been observed that planar failure is the common type of slope failure accompanied by both wedge and central failures. The plasticity index of all the soil samples is found to exceed the plastic limits of the soils when low to moderate rainfall occur. The slopes as deduced from SMR indicate instability and belong to class VIB-VIA. © 2011 CAFET-INNOVA TECHNICAL SOCIETY. All rights reserved.


Chandra Singh M.,Imphal College | Kushwaha R.A.S.,Manipur University | Srivastava G.,University of Lucknow | Mehrotra R.C.,University of Lucknow
Journal of the Geological Society of India | Year: 2012

Some plant remains are described from the Laisong Formation (Barail Group) of Thoubal and Senapati districts of Manipur. The flora is diverse comprising gymnospermous, monocotyledonous and dicotyledonous forms and indicates tropical to subtropical climate in the vicinity of the area during the deposition of the host rocks.


Singh M.C.,Imphal College | Kundal P.,Rashtrasant Tukadoji Maharaj Nagpur University | Kushwaha R.A.S.,Manipur University
Journal of the Geological Society of India | Year: 2010

Oligocene-Miocene deposits of Bhuban and Boka Bil Formations, Surma Group, Manipur Western Hill consist of well preserved ichnofossil assemblages. These formations are represented by eight lithofacies such as Massive sandstone (Sm), Rippled marked argillaceous sandstone (Sr), Wavy laminated sandstone-siltstone- silty shale (Sw), Laminated shale (Fl), Massive mudstone (Fm), Trough cross-bedded sandstone (St), Lenticular laminated sandstonesiltstonesilty shale (Sll) and Laminated to massive sandstone-siltstone (Ssc). Fifteen ichnospecies were identified, which further categories into Skolithos, Cruziana, and Skolithos/Cruziana ichnofacies. Overall distribution pattern and behavioural nature of the ichnoassemblage and sedimentological attributes suggests that the sediments of Bhuban and Boka Bil Formations were deposited under frequent fluctuating sea level, moderate to strong energy condition, subtidal to lower intertidal environment, rich in organic nutrients. © 2010 Geological Society of India.

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