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Garcia Rodriguez R.,Allergology Section | Urra J.M.,Immunology Section | Feo-Brito F.,Allergology Section | Galindo P.A.,Allergology Section | And 4 more authors.
Clinical and Experimental Allergy | Year: 2011

Background Current management of egg allergy relies on egg elimination from the diet. It does not protect patients from reactions after accidental ingestion of the food and it has a negative influence on quality of life. To solve these problems, some desensitization protocols have been described that are safe and effective, but only one study of a rush regimen for egg with a small patient sample has been published. Objective To evaluate the safety, efficacy and immunologic effects of an oral rush desensitization protocol for immediate egg allergy. Methods Subjects aged 5 years or older with symptomatic IgE-mediated allergy to hen's egg underwent a 5-day oral tolerance induction regimen and were subsequently maintained on a regular egg intake. The variables studied were the reactions that occurred during the induction regimen and follow-up and the duration of desensitization. Prick test weal size and egg white-specific IgE and IgG concentrations were monitored. Results Twenty-three patients between 5 and 17 years of age entered the protocol. Twenty (86.9%) achieved the daily intake of a whole cooked egg, 14 of them within the scheduled 5 days. One abandoned the protocol and two were changed to a slower regimen because of repeated reactions. Allergic reactions were frequent but in general were mild. No severe reactions occurred. During follow-up of at least 6 months, egg was well tolerated by all patients. Compared with baseline, skin prick test weal size and egg white-sIgE levels had fallen at 3 months, although the differences were only significant at 6 months. Conclusions and Clinical Relevance The rush protocol described is useful and safe for achieving tolerance to egg within a few days but it should always be performed in a highly supervised setting. A high proportion of patients allergic to egg can effectively be desensitized using the described schedule, with the advantage of shortening the time to become protected from reactions after inadvertent ingestion of egg, with no increase in the risk compared with the earlier reported slower protocols. © 2011 Blackwell Publishing Ltd. Source


Peix A.,Institute of Cardiology | Cabrera L.Z.,Institute of Cardiology | Heres F.,Immunology Section | Rodriguez L.,Statistics Section | And 11 more authors.
Journal of Nuclear Cardiology | Year: 2011

Coronary artery disease is a leading cause of death among diabetics, and silent ischemia is a major concern in patients with diabetes mellitus (DM). Methods. To detect the prevalence of ischemia in diabetics by myocardial perfusion imaging (MPI), and compare it to a control group without DM but with coronary risk factors, as well as to explore the relationship between silent ischemia, endothelial dysfunction, and coronary calcium, 59 patients (Group I) and 42 controls (Group II) were included. All underwent clinical and laboratory evaluations, gated MPI, brachial artery vasodilation measured by ultrasonography, and coronary calcium score (CCS). Results. Twenty diabetics showed perfusion defects, vs seven controls (P 5 .04). There was no significant difference between both groups regarding the brachial artery vasodilator responsiveness: 4.49% ± 4.26% (diabetics) vs 4.70% ± 4.98% (controls). Mean CCS was 74 in diabetics vs five in controls (P 5 .01). The only risk factor significantly associated with an abnormal MPI was the presence of diabetes (P 5 .03). In the whole population of patients and in diabetics, the abnormal endothelium-dependent vasodilation, the CCS >100, and the cholesterol/ HDL ratio >4, showed an OR >1. CCS exhibited the higher OR among the whole population: OR 2.15 [95% CI 0.42-10.99]; while for diabetics it was the cholesterol/HDL ratio: OR 3.95 [95% CI 0.71-21.84]. Conclusions. Reversible perfusion defects and coronary calcium are more frequent in diabetics. CCS, abnormal endothelium-dependent vasodilation, and cholesterol/HDL ratio higher than 4, showed an association with perfusion abnormalities in asymptomatic diabetics. Copyright © 2011 American Society of Nuclear Cardiology. Source


Muramatu L.H.,Irmandade da Santa Casa de Misericordia de Sao Paulo | Stirbulov R.,Irmandade da Santa Casa de Misericordia de Sao Paulo | Forte W.C.N.,Immunology Section
Jornal Brasileiro de Pneumologia | Year: 2013

Objective: To analyze pulmonary function parameters and pharmacodynamic response to a bronchodilator, as well as the prescription of bronchodilators, in cystic fibrosis (CF) patients. Methods: This was a retrospective cohort study involving patients 6-18 years of age, diagnosed with CF, and followed at a referral center between 2008 and 2010. We evaluated only those patients who were able to perform pulmonary function tests (PFTs). We analyzed FVC, FEV1, and FEF25-75%, expressed as percentages of the predicted values, prior to and after bronchodilator tests (pre-BD and post-BD, respectively), in 312 PFTs. Repeated measures ANOVA and multiple comparisons were used. Results: The study included 56 patients, divided into two groups: those whose PFT results spanned the 2008-2010 period (n = 37); and those whose PFT results spanned only the 2009-2010 period (n = 19). In the 2008-2010 group, there were significant reductions in post-BD FEV1 between 2008 and 2010 (p = 0.028) and between 2009 and 2010 (p = 0.036), as was also the case for pre-BD and post-BD FEF25-75% in all multiple comparisons (2008 vs. 2009; 2008 vs. 2010; and 2009 vs. 2010). In the 2009-2010 group, there were no significant differences between any of the years for any of the variables studied. Among the 312 PFTs, significant responses to the bronchodilator occurred in only 24 (7.7%), all of which were from patients for whom no bronchodilator had been prescribed during the study period. Conclusions: In the CF patients studied, there was loss of pulmonary function, indicating progressive lung disease, over time. The changes were greater for FEF25-75% than for the other variables, which suggests the initial involvement of small airways. Source


Stetson I.,University of Murcia | Stetson I.,National University of Misiones | Aviles M.,University of Murcia | Moros C.,University of Murcia | And 7 more authors.
Theriogenology | Year: 2015

The mammalian oocyte is surrounded by a matrix called the zona pellucida (ZP). This envelope participates in processes such as acrosome reaction induction, sperm binding and may be involved in speciation. In cat (. Felis catus), this matrix is composed of at least three glycoproteins called ZP2, ZP3, and ZP4. However, recent studies have pointed to the presence of a fourth protein in several mammals (rat, human, hamster or rabbit), meaning that a reevaluation of cat ZP is needed. For this reason, the objective of this research was to analyze the protein composition of cat ZP by means of proteomic analysis. Using ZP from ovaries and oocytes, several peptides corresponding to four proteins were detected, yielding a coverage of 33.17%, 71.50%, 50.23%, and 49.64% for ZP1, ZP2, ZP3, and ZP4, respectively. Moreover, the expression of four genes was confirmed by molecular analysis. Using total RNA isolated from cat ovaries, the complementary deoxyribonucleic acids encoding cat ZP were partially amplified by reverse-transcribed polymerase chain reaction. Furthermore, ZP1 was totally amplified for the first time in this species. As far as we are aware, this is the first study that confirms the presence of four proteins in cat ZP. © 2015 Elsevier Inc. Source


Singh M.,Immunology Section | Goswami T.K.,Immunology Section | Dhama K.,Avian Disease Section
Journal of Pure and Applied Microbiology | Year: 2014

Salmonellosis is a disease complex of man and animals caused by various serovars of Salmonella enterica subspecies enterica. Salmonella spp. are facultative intracellular pathogens capable of causing localized and systemic disease of significant morbidity and mortality. The present study was aimed to investigate the immune response elicited in mice following subcutaneous immunization with purified flagellin derived from Salmonella enterica serovar Typhimurium. Humoral immune response in mice was analyzed by indirect enzyme linked immunosorbent immunoassay (ELISA) wherein a significantly greater antibody response was noticed in immunized group of mice compared to the control group. Further confirmation by western blotting showed immunoreactivity of the isolated flagellin against sera obtained from flagellin immunized group on 21st day post immunization. Bacterial colony count in spleen from the mice immunized with flagellin and challenged with Salmonella intra-peritoneally showed complete clearance of the bacteria. In conclusion, bacterial flagellin demonstrated the seroconversion and protective efficacy against homologous bacterial challenge post immunization. Further explorative studies are suggested to unravel its potential application to be used as vaccine antigen or adjuvant. Source

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