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Karkouche R.,University Paris Est Creteil | Ingen-Housz-Oro S.,University Paris Est Creteil | Le Gouvello S.,Immunologie Biologique | Charlotte F.,Hopital Pitie Salpetriere | And 8 more authors.
Journal of Cutaneous Pathology | Year: 2015

Primary cutaneous aggressive epidermotropic T-cell lymphoma (PCAETCL) is a very rare lymphoma characterized by rapidly growing necrotic cutaneous lesions with an epidermotropic CD8+ T-cell neoplastic infiltrate observed histopathologically. It is associated with a very poor outcome, despite aggressive multi-agent chemotherapy. We report a 49-year-old human immunodeficiency virus (HIV)-infected patient who developed PCAETCL with associated marked vascular injury leading to diffuse purpuric and necrotic lesions complicated by recalcitrant hemophagocytic activation syndrome. The lymphoma strongly and diffusely expressed CD158k/KIR3DL2 at the protein and transcript level and NKp46 transcripts, in addition to CD8 and cytotoxic proteins. We observed a diffuse CD158k/KIR3DL2 protein expression in another case of PAETCL, not associated with immunodeficiency, which was used as a positive control. PCAETCL can develop in HIV-infected patients and may present in vasculitis-like fashion. The possible role of immunosuppression and/or HIV in oncogenesis can be postulated, as patients infected with HIV may develop anti-HIV cytotoxic CD8+ lymphoproliferations. The frequency of CD158k/KIR3DL2 and NKp46 expression in PCAECL remains to be studied in a series of cases, and may represent interesting targets for future treatments. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Francuz B.,Institute Pasteur Paris | Francuz B.,University of Paris Descartes | Demange V.,INRS | Mousel M.-L.,Institute Pasteur Paris | And 4 more authors.
Archives des Maladies Professionnelles et de l'Environnement | Year: 2014

Purpose of the study. Laboratory workers in the research sector are exposed to cleaning products, chemicals and latex; in addition to these exposures, animal care technicians are also exposed to allergens from laboratory animals. These exposures can lead to respiratory, ENT, ocular or skin symptoms, due to allergic or irritative underlying mechanisms. The aims of this study are to assess the prevalence of these symptoms, to determine the presence of specific immunoglobulins E (IgE) against these allergens and to describe the associated occupational factors in order to improve the worksite prevention program. Methods. Workers of a research institute (n = 131, 49% of whom are exposed to laboratory animals) answered medical and occupational questionnaires about their symptoms, tasks and exposures. Specific IgE against aeroallergens (Phadiatop®), laboratory animal allergens, latex and quaternary ammoniums (QA) were dosed. Lung function tests (flow-volume curves) were performed and exhaled nitric oxide measured. Results. Seventy-two workers (55%) reported at least one work-related symptom when performing tasks such as handling animals or products or while wearing latex gloves. Rhinitis is the most frequent symptom (27%), followed by conjunctivitis (23%), dry cough (21%), sore throat (14%), eczema (11%) and asthma (2%). Monthly task duration is longer in symptomatic workers. Thirteen workers (21% among those 63 in whom it was determined) had specific IgE against an allergen from at least one laboratory animal. Specific IgE prevalence against latex on one hand and QA on the other hand were found to be the same: 4% (n = 5). Atopy is a contributing factor to sensitization against laboratory animals. Lung function tests show bronchial obstruction in 6 workers. Exhaled nitric oxide concentration is higher in symptomatic than in asymptomatic workers. Workers are exposed to several agents (detergents/ disinfectants, culture media products and animals), which induce concomitant allergic or irritative effects. Conclusion. Given this high prevalence of symptoms in exposed workers, specially rhinitis that may precede the occurrence of asthma, a prevention program has been strengthened in order to substitute, when possible, some of the chemicals, to reduce exposure levels to allergens and irritants, and to improve the use of personal protection equipment as well as the compliance to the use of detergents/disinfectants and culture media products. © 2013 Elsevier Masson SAS. All rights reserved.

Rosain J.,Immunologie Biologique | Ngo S.,Immunologie Biologique | Bordereau P.,Immunologie Biologique | Poulain N.,Immunologie Biologique | And 6 more authors.
Annales de Biologie Clinique | Year: 2014

The complement system is a complex system involving serum and membrane proteins interacting in a regulated manner. The complement system plays a major role in antibacterial immunity, in inflammation, and in immune complex processing. Therefore, deficiencies in complement proteins are associated with increased susceptibility to bacterial infections and autoimmune diseases. These deficiencies can be inherited or acquired. Most of them can be screened by simple laboratory tests but require a diagnosis in a specialized laboratory. All sequences of complement genes are known, and the discovery of a deficiency must lead to genetic testing. The discovery of a congenital deficiency requires a familial study and a prophylaxis. In this article, we review the complement cascade, the laboratory tests to explore it, and the main diseases associated with complement deficiencies.

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