Immunodiagnostic Systems Ltd IDS Ltd

Newcastle upon Tyne, United Kingdom

Immunodiagnostic Systems Ltd IDS Ltd

Newcastle upon Tyne, United Kingdom
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Bartels E.M.,Copenhagen University | Christensen R.,Copenhagen University | Christensen R.,University of Southern Denmark | Christensen P.,Copenhagen University | And 6 more authors.
Osteoarthritis and Cartilage | Year: 2014

Objective: Changes in biomarkers for bone and cartilage in knee osteoarthritis (KOA) may reflect changes in tissue turnover induced by interventions. The aim of this study was to assess the effect on osteoarthritis biomarkers of an intensive weight loss intervention in obese KOA patients. Methods: 192 obese KOA patients followed a 16 weeks weight loss intervention (ClinicalTrials.gov: NCT00655941). Serum Cartilage Oligomeric Matrix Protein (sCOMP), Urine C-terminal telopeptide of collagen type II (uCTX-II) and type I (uCTX-I) were determined by enzyme-linked immunoassay (ELISA) at baseline and after 16 weeks. Patient-reported symptoms were assessed by the Knee Injury and Osteoarthritis Outcome Score (KOOS) Questionnaire without the sports and recreation score (KOOS-4). Change from baseline was analyzed using Analysis of CoVariance (ANCOVA) adjusting for sex, age, and body mass index (BMI). Bivariate associations were analyzed using Spearman's test of rank correlation. Results: 175 patients completed the treatment and lost mean 13.4 (95% CI: 12.5-14.4) kg. sCOMP concentration decreased on average 1.1 (95% CI:-1.5 to-0.8) U/L with a correlation to weight loss (r=-0.17, P=0.028), but not to change in KOOS-4 (r=-0.13, P=0.091). uCTX-II increased significantly, mean 69 (95% CI: 31-106) ng/mmol creatinine, with no relation to weight loss (P=0.14). Change in uCTX-II was reversely related to change in KOOS-4 (r=-0.28, P=0.0003). uCTX-I increased, mean 67 (95% CI: 47-87) μg/mmol creatinine, and correlated to weight loss (r=0.22, P=0.0007), while not to KOOS-4 (P=0.93). Conclusion: A rapid substantial weight loss in obese KOA patients was weakly, while significantly associated with a reduction in sCOMP, and increases in both uCTX-II and uCTX-I. © 2014 Osteoarthritis Research Society International.


PubMed | University of Aalborg, University of Southern Denmark, Immunodiagnostic Systems Ltd IDS and Copenhagen University
Type: Clinical Trial | Journal: Osteoarthritis and cartilage | Year: 2014

Changes in biomarkers for bone and cartilage in knee osteoarthritis (KOA) may reflect changes in tissue turnover induced by interventions. The aim of this study was to assess the effect on osteoarthritis biomarkers of an intensive weight loss intervention in obese KOA patients.192 obese KOA patients followed a 16 weeks weight loss intervention (ClinicalTrials.gov: NCT00655941). Serum Cartilage Oligomeric Matrix Protein (sCOMP), Urine C-terminal telopeptide of collagen type II (uCTX-II) and type I (uCTX-I) were determined by enzyme-linked immunoassay (ELISA) at baseline and after 16 weeks. Patient-reported symptoms were assessed by the Knee Injury and Osteoarthritis Outcome Score (KOOS) Questionnaire without the sports and recreation score (KOOS-4). Change from baseline was analyzed using Analysis of CoVariance (ANCOVA) adjusting for sex, age, and body mass index (BMI). Bivariate associations were analyzed using Spearmans test of rank correlation.175 patients completed the treatment and lost mean 13.4 (95% CI: 12.5-14.4) kg. sCOMP concentration decreased on average 1.1 (95% CI:-1.5 to-0.8) U/L with a correlation to weight loss (r=-0.17, P=0.028), but not to change in KOOS-4 (r=-0.13, P=0.091). uCTX-II increased significantly, mean 69 (95% CI: 31-106) ng/mmol creatinine, with no relation to weight loss (P=0.14). Change in uCTX-II was reversely related to change in KOOS-4 (r=-0.28, P=0.0003). uCTX-I increased, mean 67 (95% CI: 47-87) g/mmol creatinine, and correlated to weight loss (r=0.22, P=0.0007), while not to KOOS-4 (P=0.93).A rapid substantial weight loss in obese KOA patients was weakly, while significantly associated with a reduction in sCOMP, and increases in both uCTX-II and uCTX-I.


Koivula M.-K.,University of Oulu | Richardson J.,Immunodiagnostic Systems Ltd IDS Ltd | Leino A.,University of Turku | Valleala H.,University of Helsinki | And 7 more authors.
Clinical Biochemistry | Year: 2010

ObjectivesN-terminal propeptide of type I procollagen assay (PINP) reflects the rate of type I collagen synthesis. Design and methodsDifferent sera were fractioned by gel filtration and analyzed with intact and total PINP assays. The sizes of the antigens were determined by western blotting. The thermal stability was tested at + 37 °C, + 4 °C and room temperature (RT). ResultsAutomated intact PINP assay hardly measured monomeric form. In haemodialysis patients intact and total PINP assays gave significantly different results. The monomeric PINP antigen in serum was larger than the trimeric PINP antigen. PINP were thermally stable at least 7 days at + 4 °C and at RT but the results of both assays were decreased similarly at +. 37°C. ConclusionsThe IDS-iSYS intact PINP assay is precise and sensitive. It seems that monomeric form is not derived from the thermal instability of the trimers but acts as a confounding factor. © 2010 The Canadian Society of Clinical Chemists.

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