The Immune Disease Institute and Program in Cellular and Molecular Medicine Childrens Hospital Boston

Head of Westport, MA, United States

The Immune Disease Institute and Program in Cellular and Molecular Medicine Childrens Hospital Boston

Head of Westport, MA, United States
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Whitney J.B.,Beth Israel Deaconess Medical Center | Whitney J.B.,Harvard University | Mirshahidi S.,Loma Linda University | Lim S.,Beth Israel Deaconess Medical Center | And 11 more authors.
Journal of Immune Based Therapies and Vaccines | Year: 2011

Background: We have evaluated an attenuated Listeria monocytogenes (Lm) candidate vaccine vector in nonhuman primates using a delivery regimen relying solely on oral vaccination. We sought to determine the impact of prior Lm vector exposure on the development of new immune responses against HIV antigens.Findings: Two groups of rhesus macaques one Lm naive, the other having documented prior Lm vector exposures, were evaluated in response to oral inoculations of the same vector expressing recombinant HIV-1 Gag protein. The efficacy of the Lm vector was determined by ELISA to assess the generation of anti-Listerial antibodies; cellular responses were measured by HIV-Gag specific ELISpot assay. Our results show that prior Lm exposures did not diminish the generation of de novo cellular responses against HIV, as compared to Listeria-naïve monkeys. Moreover, empty vector exposures did not elicit potent antibody responses, consistent with the intracellular nature of Lm.Conclusions: The present study demonstrates in a pre-clinical vaccine model, that prior oral immunization with an empty Lm vector does not diminish immunogenicity to Lm-expressed HIV genes. This work underscores the need for the continued development of attenuated Lm as an orally deliverable vaccine. © 2011 Whitney et al; licensee BioMed Central Ltd.

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