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Bhubaneshwar, India

Paital B.,Utkal University | Paital B.,Banaras Hindu University | Kumar S.,Institute of Life science | Farmer R.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | And 2 more authors.
Interdisciplinary Sciences: Computational Life Sciences | Year: 2013

In the present study, we used computational methods to model crab and rat MnSOD using the crystal structure of MnSOD from Homo sapiens (PDB code: 1MSD) as template by comparative modeling approach. We performed molecular dynamics simulations to study dynamic behavior of the crab MnSOD. The modeled proteins were validated and subjected to molecular docking analyses. Molecular docking tool was used to elucidate a comparative binding mode of the crab and rat SOD with potent inhibitors of SOD such as hydrogen peroxide (H2O2), potassium cyanide (KCN) and sodium dodecyl sulphate (SDS). The predicted valid structure of crab MnSOD did not show any interaction with KCN but close interaction with H2O2 and SDS. A possible inhibitory mechanism of SDS and H2O2 due to their interaction with the amino acids present in the active site of the MnSOD of the above two animals are elucidated. This allowed us to predict the binding modes of the proteins to elucidate probable mode of action and sites of interference. © 2013 International Association of Scientists in the Interdisciplinary Areas and Springer-Verlag Berlin Heidelberg. Source

Jena S.,Utkal University | Anand C.,Utkal University | Chainy G.B.N.,Imgenex India Pvt. Ltd. | Dandapat J.,Utkal University
Neurological Sciences | Year: 2012

The present study was carried out to elucidate the effectiveness of curcumin in ameliorating the expression of superoxide dismutase (SOD) in cerebral cortex and cerebellum of rat brain under 6-propyl-2-thiouracil (PTU)- induced hypothyroidism. Induction of hypothyroidism in adult rats by PTU resulted in augmentation of lipid peroxidation (LPx), an index of oxidative stress in cerebellum but not in cerebral cortex. Curcumin-supplementation to PTU-treated (hypothyroid) rats showed significant reduction in the level of LPx in both the regions of brain. The decreased translated products (SOD1 and SOD2) and the unchanged activity of SOD in cerebral cortex of PTUtreated rats were increased on supplementation of curcumin to the hypothyroid rats. Declined translated products of SOD1 and SOD2 in cerebellum of PTU-treated rats were alleviated on administration of curcumin to hypothyroid rats. On the other hand, the decreased activity of SOD in cerebellum of PTU-treated rats was further declined on administration of curcumin to the hypothyroid rats. Results of the present investigation indicate that curcumin differentially modulates the expression of superoxide dismutase in rat brain cortex and cerebellum under PTU-induced hypothyroidism. © 2011 Springer-Verlag. Source

Ray M.,Siksha O' Anusandhan University | Dash S.,Siksha O' Anusandhan University | Shahbazi S.,Panjab University | Achary K.G.,Imgenex India Pvt. Ltd. | And 2 more authors.
LWT - Food Science and Technology | Year: 2016

Early and precise diagnosis of diseases in turmeric (Curcuma longa L.) permits early treatment which can enhance yield and quality of Indian spices. In the present study, polyclonal antibody (pAb) raised against the protein extract from Pythium aphanidermatum was established for the detection of rhizome rot using serological assays. The fungal proteins were recognized by ELISA with high sensitivity (50 ng) and the proteins ranging from 15 to 95 kDa showed immuno-reactivity in Western blot using the developed pAb. Correlation coefficient between infected samples at various stages from different agroclimatic zones with antibody titres was taken as the primary endpoint for standardization of the protocol. Highest positive correlation (r = 0.999) was observed in stage I and II infected samples of North-western zone, whereas low negative correlation (r = -0.284) was found in stage III infected samples of Western zone with developed pAb. Linear positive correlations (R2) exist between antigenic proteins at all the stages of infection and the developed pAb titres. Hence, the developed pAbs could be used to detect the presence of the fungal pathogen at an early stage. These serological approaches allow us to develop a rapid, sensitive, specific and accurate diagnostic kit for early detection of rhizome rot in turmeric. © 2015 Elsevier Ltd. Source

Paital B.,Utkal University | Kumar S.,Institute of Life science | Farmer R.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Tripathy N.K.,Berhampur University | And 2 more authors.
Interdisciplinary Sciences: Computational Life Sciences | Year: 2011

The enzyme catalase breaks down H2O2, a potentially harmful oxidant, to H2O and O2. Besides oxidase activity, the enzyme also exhibits peroxidase activity. Therefore, it plays an important role in maintaining health and regulating pathophysiology of the organisms. However, 3D structure of this important enzyme in invertebrates particularly in crabs is not yet available. Therefore, an attempt has been made to predict the structure of the crab catalase and to envisage its catalytic interaction with H2O2. A three dimensional model of crab catalase was constructed using the NADPH binding site on Beef Liver catalase from Bos taurus (PDBID: 7CAT) as template by comparative modeling approach. Backbone conformation of the modeled structure by PROCHECK revealed that more than 98% of the residues fell in the allowed regions, ERRAT results confirmed good quality of modeled structure and VERIFY3D profile was satisfying. Molecular docking has been used to know the binding modes of hydrogen peroxide with the crab catalase protein. The receptor structures used for docking were derived from molecular dynamics (MD) simulations of homology modeled structure. The docking results showed that the three important determinant residues Arg68, Val70 and Arg108 in catalase were binding with H2O2 as they had strong hydrogen bonding contacts with the substrate. Our analysis provides insight into the structural properties of crab catalase and defines its active sites for binding with substrate. These data are important for further studies of catalase of invertebrates in general and that of crabs in particular. © 2011 International Association of Scientists in the Interdisciplinary Areas and Springer-Verlag Berlin Heidelberg. Source

Dash C.,Orissa University of Agriculture and Technology | Mohapatra S.B.,Orissa University of Agriculture and Technology | Maiti P.K.,Imgenex India Pvt. Ltd.
Preparative Biochemistry and Biotechnology | Year: 2016

Actinobacteria are promising source of a wide range of important enzymes, some of which are produced in industrial scale, with others yet to be harnessed. L-Asparaginase is used as an antineoplastic agent. The present work deals with the production and optimization of L-asparaginase from Actinomycetales bacterium BkSoiiA using submerged fermentation in M9 medium. Production optimization resulted in a modified M9 medium with yeast extract and fructose as carbon and nitrogen sources, respectively, at pH 8.0, incubated for 120 hr at 30 ± 2°C. The crude enzyme was purified to near homogeneity by ammonium sulfate precipitation following dialysis, ion-exchange column chromatography, and finally gel filtration. The sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) revealed an apparent molecular weight of 57 kD. The enzyme was purified 95.06-fold and showed a final specific activity of 204.37 U/mg with 3.49% yield. The purified enzyme showed maximum activity at a pH 10.0 and was stable at pH 7.0 to 9.0. The enzyme was activated by Mn2+ and strongly inhibited by Ba2+. All these preliminary characterization suggests that the L-asparaginase from the source may be a tool useful to pharmaceutical industries after further research. Copyright © 2016 Taylor & Francis Group, LLC. Source

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