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Idir M.,Brookhaven National Laboratory | Dovillaire G.,Imagine Optic Inc. | Mercere P.,Synchrotron Soleil
Synchrotron Radiation News | Year: 2013

In recent years, there has been growing interest in the design of electron accelerators in order to reduce beam emittance and to increase photon brilliance (from third-generation synchrotron sources to free electron lasers). This has increased the coherent properties of the beam and has opened up new branches of microscopy and spectroscopy at nanometer-length scales. The X-ray nano probe is going to be an important tool for future research, hence there has been substantial research carried out in order to develop nano focusing optics of diffraction-limited performance. © 2013 Copyright Taylor and Francis Group, LLC.

Idir M.,Brookhaven National Laboratory | Kaznatcheev K.,Brookhaven National Laboratory | Dovillaire G.,Imagine Optic Inc. | Legrand J.,Imagine Optic Inc. | Rungsawang R.,Imagine Optic Inc.
Optics Express | Year: 2014

We present a 2D Slope measuring System based on a Stitching Shack Hartmann Optical Head (SSH-OH) aiming to perform high accuracy optical metrology for X-ray mirrors. This system was developed to perform high-accuracy automated metrology for extremely high quality optical components needed for synchrotrons or Free Electrons Lasers (FEL), EUV lithography and x-ray astronomy with slope error accuracy better than 50 nrad rms. © 2014 Optical Society of America.

Izeddin I.,University Pierre and Marie Curie | Izeddin I.,Ecole Normale Superieure de Paris | El Beheiry M.,University Pierre and Marie Curie | Andilla J.,Imagine Optic Inc. | And 4 more authors.
Optics Express | Year: 2012

We present a novel approach for three-dimensional localization of single molecules using adaptive optics. A 52-actuator deformable mirror is used to both correct aberrations and induce two-dimensional astigmatism in the point-spread-function. The dependence of the z-localization precision on the degree of astigmatism is discussed. We achieve a z-localization precision of 40 nm for fluorescent proteins and 20 nm for fluorescent dyes, over an axial depth of ∼800 nm. We illustrate the capabilities of our approach for three-dimensional high-resolution microscopy with superresolution images of actin filaments in fixed cells and single-molecule tracking of quantum-dot labeled transmembrane proteins in live HeLa cells. © 2012 Optical Society of America.

Hosy E.,Institut Universitaire de France | Hosy E.,French National Center for Scientific Research | Butler C.,Institut Universitaire de France | Butler C.,French National Center for Scientific Research | And 3 more authors.
Current Opinion in Chemical Biology | Year: 2014

Progresses in microscopy have often led to major discoveries in neuroscience, and the recent advent of super-resolution microscopy is no exception. In this review, we will show how imaging has advanced our modern vision of synaptic function. More particularly, we will emphasize how novel nanoscopy techniques have helped in deciphering the organization of post-synaptic proteins, offering new insight into the mechanism of synaptic transmission. © 2014 Elsevier Ltd.

Agency: European Commission | Branch: H2020 | Program: RIA | Phase: FETOPEN-1-2014 | Award Amount: 4.00M | Year: 2015

Computerized Tomography (CT) has been one of the greatest achievements in medical imaging, but at the cost of a high, potentially harmful, X-ray irradiation dose. The ultimate goal of VOXEL is to provide an alternative to tomography with a disruptive technology enabling 3D X-ray imaging at very low dose. VOXEL aims at prototyping new cameras that will combine the X-ray penetration and nanometre spatial resolution, easiness to use, afforded by avoiding the rotation of the source or the sample, and extremely low dose for maximum impact on medicine and biology. VOXEL relies on the integration of trans-disciplinary fields in medical imaging, optics, X-ray physics, applied mathematics and value to society through foreseeable commercialization. VOXEL aims at prototyping in parallel a soft X-ray water window microscope and a hard X-ray 3D camera for medical applications. While both cameras need groundbreaking development in the underlying physics, only hard X-ray camera has high technological risk (and high societal impact). VOXEL will benefit from the soft X-ray camera thanks to its Biological applications in nano-tomography but also as a test platform for our physical and mathematical models.. The VOXEL team members are leaders in X-ray metrology, wavefront sensing, atomic physic, mathematical computing and 3D medical imaging; with VOXEL we are uniquely positioned to succeed, and to raise the competitiveness of Europe. Doing so by basing the research lead in Portugal with a woman coordinator will be exemplary: beyond the scientific and technological success, thanks to our focus in science and its valorisation, VOXEL will be transformative for scientifically emerging countries.

Imagine Optic Inc. and French National Center for Scientific Research | Date: 2014-08-27

According to one aspect, the invention concerns a method for microscopy of a thick sample arranged on a sample support, with edge-illumination of the sample. The method comprises, in particular, emitting at least one illumination beam (1), forming, from the illumination beam, an illumination surface, focusing the illumination surface in the sample by means of a microscope lens (120) and deflecting the illumination surface originating from the microscope lens, in order to form a transverse illumination surface, located in a plane substantially perpendicular to the optical axis of the microscope lens. The method further comprises forming, by means of said microscope lens (120), the image of an area of the sample illuminated by the transverse illumination surface on a detection surface (131) of a detection device (130), scanning the illumination beam, allowing the transverse illumination surface to move along the optical axis of the microscope lens, and superimposing the object imaging surface and the transverse illumination surface, by focusing means comprising means separate from the means for the relative axial movement of the microscope lens and the sample.

Cha J.W.,Massachusetts Institute of Technology | Ballesta J.,Imagine Optic Inc. | So P.T.C.,Massachusetts Institute of Technology
Journal of Biomedical Optics | Year: 2010

The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration. © 2010 Society of Photo- Optical Instrumentation Engineers.

Agency: European Commission | Branch: FP7 | Program: MC-IAPP | Phase: PEOPLE-2007-3-1-IAPP | Award Amount: 524.76K | Year: 2008

The development of high resolution, non damaging imaging techniques are crucial for understanding the biological processes occurring at the cellular level. Nonlinear microscopy (NLM) is rapidly establishing as a powerful technique for high resolution imaging of living biological samples. The high peak powers and low pulse energies available from ultrashort pulses, allow an efficient excitation of nonlinear effects with reduced collateral damage when interacting with cells. Ultrashort pulse light has three additional parameters that can be exploited for a more efficient light-cell interaction. i) Pulse shaping, acting on the temporal intensity profile and phase parameters of a pulse will allow for a more efficient and less damaging interaction with the sample. ii) Adaptive optics will be used to modulate the beams wavefront spatial distribution parameter. This will allow correcting aberrations for an increased transversal resolution, larger penetration depths and fields of view. As focus spot size is reduced, it will also allow for lower power to be used, preserving living specimens. In this project, we aim, for the first time, to join complementary intersectorial expertise to perform simultaneous pulse shaping and wavefront correction at the sample plane of a NLM. The knowledge of each of the teams will be transferred, based on microscopy applications. Firstly the work will be focused towards implementing adaptive optics to minimise aberrations induced by the setup and by the sample of study. Then pulse shaping will be use to enhance the output signal and reduce side effects (such as photobleaching). Finally, the combined action will result on new contrast generation technique, constituting an important breakthrough in NLM with great implications into bio-medicine.

A super-resolution microscopy method includes forming an image of an emitting particle in a detection plane of a detector by a microscopy imaging system and correcting, by a wavefront-modulating device, at least some of the optical defects present between the emitting particle and the detection plane. The method further includes introducing, via the wavefront-modulating device, a deformation of the wavefront emitted by the emitting particle, of variable amplitude, allowing a bijective relationship to be formed between the shape of the image of the emitting particle in the detection plane and the axial position of the emitting particle relative to an object plane that is optically conjugated with the detection plane by the microscopy imaging system. The method further includes controlling the amplitude of the deformation of the wavefront by controlling the wavefront-modulating device, as a function of the given range of values of the axial position of the particle.

Methods and devices for reducing the dimensions of an incident light beam of large dimensions are disclosed. The method includes the dispatching of a first light beam toward a partially reflecting plate of dimensions suitable for the dimensions of the light beam of large dimensions, the dispatching onto a convergent reflective element of a second light beam arising from the transmission through the partially reflecting plate of the first light beam, the dispatching of a third light beam arising from the reflection on the convergent reflective element of the second light beam, toward said partially reflecting plate, and the reflection of the third beam on the partially reflecting plate so as to form a fourth light beam.

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