Lee K.,Dongguk University |
Ban H.S.,Korea Research Institute of Bioscience and Biotechnology |
Naik R.,Dongguk University |
Hong Y.S.,Korea Research Institute of Bioscience and Biotechnology |
And 6 more authors.
Angewandte Chemie - International Edition | Year: 2013
Tracking the target: To identify a protein that binds directly to the HIF-1α inhibitor LW6, a series of new chemical probes were synthesized with a clickable tag and/or a photoactivatable moiety. LW6 was found to be localized to the mitochondria (see picture) and MDH2 was identified as a target protein of LW6. These results indicate that the HIF-1α inhibitory activity of LW6 is a consequence of MDH2 suppression. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source
Kwon H.-S.,Seoul National University |
Kwon H.-S.,ILDONG Pharmaceutical Co. |
Park M.C.,Seoul National University |
Kim D.G.,Seoul National University |
And 5 more authors.
Journal of Cell Science | Year: 2012
Aminoacyl-tRNA-synthetase-interacting multifunctional protein 1 (AIMP1/p43) can be secreted to trigger proinflammatory molecules while it is predominantly bound to a cytoplasmic macromolecular protein complex that contains several different aminoacyl-tRNA synthetases. Although its activities as a secreted signaling factor have been well characterized, the functional receptor for its proinflammatory activity has not yet identified. In this study, we have identified the receptor molecule for AIMP1 that mediates the secretion of TNF-α from THP-1 monocytic cells and primary human peripheral blood mononuclear cells (PBMCs). In a screen of 499 soluble receptors we identified CD23, a known low-affinity receptor for IgE, as a high affinity binding partner of AIMP1.We found that downregulation of CD23 attenuated AIMP1-induced TNF-α secretion and AIMP1 binding to THP-1 and PBMCs. We also observed that in THP-1 and PBMCs, AIMP1-induced TNF-α secretion, mediated by CD23, involved activation of ERK1/2. Interestingly, endothelial monocyte activating polypeptide II (EMAP II), the Cterminal fragment of AIMP1 that is also known to work as a proinflammatory cytokine, was incapable of binding to CD23 and of activating ERK1/2. Therefore, identification of CD23 not only explains the inflammatory function of AIMP1 but also provides the first evidence by which the mode of action of AIMP1 can be distinguished from that of its C-terminal domain, EMAP II. © 2012. Source
Park S.,ILDONG Pharmaceutical Co. |
Kim D.,University of Seoul |
Jang I.,Sogang University |
Oh H.B.,Sogang University |
Choe J.,University of Seoul
Biochemical and Biophysical Research Communications | Year: 2014
Most bacteria have developed a hemoprotein degradation system to acquire iron from their hosts. Bacillus subtilis HmoB, a heme monooxygenase, is involved in the degradation of heme and subsequent release of iron. HmoB contains a C-terminal ABM domain, which is similar in sequence and structure to other heme monooxygenases. Heme degradation assay showed that highly conserved residues (N70, W128, and H138) near the heme-binding site were critical for activity of HmoB. However, HmoB was shown to be different from other bacterial heme oxygenases due to its longer N-terminal region and formation of a biological monomer instead of a dimer. The degradation product of B. subtilis HmoB was identified as staphylobilin from mass spectrometric analysis of the product and release of formaldehyde during degradation reaction. © 2014 Elsevier Inc. All rights reserved. Source
Ildong Pharmaceutical Co. | Date: 2016-06-21
Dermal filler kits comprised of a hyaluronic acid gel in a pre-filled syringe, injected under the skin, used to reduce or eliminate fine lines and wrinkles, fill scars and contour the lips and face; fillers for dermatological use; dermal filler kits for medical purposes; dermal filler injection system for medical purposes; skin fillers; dermal implants, including viscosity supplements for medical use, for filling in wrinkles or increasing volume; pectoral implants; dental instruments; contraceptive devices; skin esthetic massaging apparatus; whitening apparatus for skin esthetic; skin esthetic apparatus; clothing especially for operating rooms; gloves for medical purposes; surgical masks; massaging apparatus for personal use; orthopedic articles; medical apparatus and instruments.
Ildong Pharmaceutical Co. | Date: 2014-10-20
Vitamin preparations; Antibiotics; Anti-cancer preparations; Antidepressants; Digestives for pharmaceutical purposes; Cardiovascular pharmaceuticals; Pharmaceutical preparations which contain lactobacillus or probiotic compositions for the treatment of intestinal disorders; Lactose for pharmaceutical purposes; Culture fluids for cultivating human, animal, plant or microorganism cells; Culture media for cultivating human, animal, plant or microorganism cells.