New Haven, CT, United States
New Haven, CT, United States
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An automated method of screening for cervical cancer cells, comprising the steps of:(a) obtaining a slide containing a fluid biological sample comprising nuclei from a subject, said biological sample having been contacted with a first nuclear centromeric probe bearing a first detectable label directed either to chromosome 3 or chromosome 7, and a second nuclear non-centromeric probe bearing a second detectable label directed to single copy sequences on the q arm of chromosome 3 wherein said single copy sequences comprise a contiguous sequence of four overlapping BAC clones containing at least a portion of the TERC gene at chromosomal location 3q26, and counterstained with DAPI, wherein said first and second probes are contacted under conditions that promote hybridization of each said first and second probe to the respective chromosomal loci;(b) searching said slide for DAPI stained areas within said biological sample;(c) searching said DAPI stained areas for the presence of label of said first nuclear centromeric probe and label of said second nuclear non-centromeric probe;(d) quantitatively determining the number of said first nuclear centromeric probe and second nuclear non-centromeric probe per nucleus in each DAPI stained area within said biological sample;(e) designating the biological sample as comprising a potential for progression to high hyperplasia and invasive cervical cancer when the number of said first probe per nucleus within the DAPI is about 2 per nucleus and the number of said second nuclear non-centromeric probe per nucleus within the DAPI stained area is equal to or greater than 4.


A method for automated microscopic analysis wherein the test protocol is obtained from interrogatable data affixed to each microscope slide. The method further comprises the algorithms that implement the test protocol.


A method for automated microscopic analysis wherein the test protocol is obtained from interrogatable data affixed to each microscope slide. The method further comprises the algorithms that implement the test protocol.


Patent
Ikonisys | Date: 2014-06-27

An improved filter wheel is disclosed, through embodiments, that comprises a plurality of reflector support structures formed as a unitized structure with a circular base member. In some embodiments, the filter wheel further comprises a plurality of light sources mounted on the filter wheel.


Patent
Ikonisys | Date: 2013-03-19

An improved filter wheel is disclosed, through embodiments, that comprises a plurality of reflector support structures formed as a unitized structure with a circular base member. In some embodiments, the filter wheel further comprises a plurality of light sources mounted on the filter wheel.


Patent
Ikonisys | Date: 2013-09-09

In various embodiments methods for automated screening for gene amplification in biological tissue samples using an automated fluorescence microscope to analyze fluorescence in situ hybridized samples are provided. Various additional embodiments provide methods of high throughput screening for gene amplification.


A method for providing quantitative information regarding the extent of infection of host cells by an infectious agent. A microscope image of a specimen of a bodily fluid is analyzed using image processing techniques to quantify the percentage of the area of the specimen that is infected.


Automated methods for detecting cancer and related hyperplasias in biological samples.


A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.


Patent
Ikonisys | Date: 2013-10-25

A system and method for remote control of an automated microscope via a widely distributed network.

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