PubMed | IIS Inc and University of Kiel
Type: Journal Article | Journal: Journal of the American Society of Nephrology : JASN | Year: 2016
AKI is histologically characterized by necrotic cell death and inflammation. Diverse pathways of regulated necrosis have been reported to contribute to AKI, but the molecular regulators involved remain unclear. We explored the relative contributions of ferroptosis and necroptosis to folic acid (FA)-induced AKI in mice. FA-AKI in mice associates with lipid peroxidation and downregulation of glutathione metabolism proteins, features that are typical of ferroptotic cell death. We show that ferrostatin-1 (Fer-1), an inhibitor of ferroptosis, preserved renal function and decreased histologic injury, oxidative stress, and tubular cell death in this model. With respect to the immunogenicity of ferroptosis, Fer-1 prevented the upregulation of IL-33, an alarmin linked to necroptosis, and other chemokines and cytokines and prevented macrophage infiltration and Klotho downregulation. In contrast, the pancaspase inhibitor zVAD-fmk did not protect against FA-AKI. Additionally, although FA-AKI resulted in increased protein expression of the necroptosis mediators receptor-interacting protein kinase 3 (RIPK3) and mixed lineage domain-like protein (MLKL), targeting necroptosis with the RIPK1 inhibitor necrostatin-1 or genetic deficiency of RIPK3 or MLKL did not preserve renal function. Indeed, compared with wild-type mice, MLKL knockout mice displayed more severe AKI. However, RIPK3 knockout mice with AKI had less inflammation than their wild-type counterparts, and this effect associated with higher IL-10 concentration and regulatory T cell-to-leukocyte ratio in RIPK3 knockout mice. These data suggest that ferroptosis is the primary cause of FA-AKI and that immunogenicity secondary to ferroptosis may further worsen the damage, although necroptosis-related proteins may have additional roles in AKI.
Vivanco F.,IIS Inc |
Vivanco F.,Complutense University of Madrid |
Barderas M.G.,Hospital Nacional Of Paraplejicos |
Laborde C.M.,Hospital Nacional Of Paraplejicos |
And 4 more authors.
Journal of Biomedicine and Biotechnology | Year: 2011
Metabolomics involves the identification and quantification of metabolites present in a biological system. Three different approaches can be used: metabolomic fingerprinting, metabolic profiling, and metabolic footprinting, in order to evaluate the clinical course of a disease, patient recovery, changes in response to surgical intervention or pharmacological treatment, as well as other associated features. Characteristic patterns of metabolites can be revealed that broaden our understanding of a particular disorder. In the present paper, common strategies and analytical techniques used in metabolomic studies are reviewed, particularly with reference to the cardiovascular field. © 2011 Maria G. Barderas et al.
Zheng B.,IIS Inc |
Sun Y.,Brother Industries |
Takamatsu J.,NAIST |
Ikeuchi K.,IIS Inc
IPSJ Transactions on Computer Vision and Applications | Year: 2013
In this paper, we propose a novel local image descriptor DoP which is termed as the difference of images represented by polynomials in different degrees. Once an interest point/region is extracted by a common image detector such as Harris corner, our DoP descriptor is able to characterize the interest point/region with high distinctiveness, compactness, and robustness to viewpoint change, image blur, and illumination variation. To efficiently build DoP descriptor, we propose to numerically reduce the computational cost by jumping over the repeatedly calculating polynomial representation. Our experimental results demonstrate a better performance compared to several state-of-art candidates. © 2013 Information Processing Society of Japan.
Makino H.,NILIM |
Kamijo S.,IIS Inc
IEICE Transactions on Fundamentals of Electronics, Communications and Computer Sciences | Year: 2015
ITS R&D includes wide variety of research area such as mechanical engineering, road engineering, traffic engineering, information and communication engineering, and electrical engineering. In spite of initiatives across the variety of engineering is essential to solve the problems of practical social systems, it is difficult to collaborate among engineering. Based on the joint research of the Japan Society of Civil Engineers and the Institute of Electrical Engineers held at the Great East Japan Earthquake, this paper discusses about necessity of collaboration among academies on ITS R&D. International collaboration is also important for ITS R&D. Asian countries could share the same problems and solutions, since many of mega cities exist in Asia region and they suffers from heavy traffics. Therefore, we need to discuss the common solution to our problems. Copyright © 2015 The Institute of Electronics, Information and Communication Engineers.
Fukuba T.,University of Tokyo |
Aoki Y.,University of Tokyo |
Fukuzawa N.,University of Tokyo |
Yamamoto T.,Tokyo Institute of Technology |
And 3 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2011
We have developed and tested a functionally integrated in situ analyzer, the IISA-ATP system, for microbial activity assays based on a quantitative determination of the total (particulate and dissolved) ATP in ocean environments. The IISA-ATP utilizes a PDMS-glass hybrid microfluidic device as its core functional element, which can perform cell lysis and total ATP quantification by a luciferin-luciferase bioluminescence assay in situ. Transparent heaters and a temperature sensor fabricated on a glass substrate provide temperature control. As a result of the evaluation using the microfluidic device with ATP standard solutions, the bioluminescence intensity was linearly correlated with 2 × 10-12 to 2 × 10 -8 M of ATP. A detection limit of 1.1 × 10-11 M was determined using the completed IISA-ATP system, which includes a miniature pumping module and a control module. As a result of the evaluation using the environmental seawater sample collected from Tokyo Bay, Japan, 2.7 × 10-10 M of total ATP was successfully determined in the laboratory by the IISA-ATP. The system was operated at a shallow submarine hot spring area in Okinawa, Japan for an in situ trial. The result shows the system was successfully operated in situ and the total ATP was determined to be 3.4 × 10-10 M. This journal is © The Royal Society of Chemistry.
Cheng C.,IIS Inc |
Cheng C.,University of Tokyo |
Kawaguchi K.,IIS Inc
Measurement: Journal of the International Measurement Confederation | Year: 2015
Steel structures had experienced an intense and long transient response caused by the great earthquake motion during the Great East Japan Earthquake on 11th March 2011. Lots of non-structural damages such as ceiling fallen and glass broken are the main damage characteristics of steel structures although there were rarely structural damages. Some opened earthquake records and videos were adopted in this study in order to investigate the seismic behavior of the steel structures. An application of vision-based method has been carried out on a video of surveillance camera provided by Japan Broadcasting Corporation (Nippon Housou Kyoukai) to obtain the displacement response and natural period of the high-rise steel buildings in Tokyo. © 2014 Elsevier Ltd. All rights reserved.
Visser R.G.,IIS Inc
Accreditation and Quality Assurance | Year: 2016
The tentative arguments mentioned by Paul De Bievre are discussed and alternatives are given that may lead to different insights. © 2016 Springer-Verlag Berlin Heidelberg
Kasyanov V.,IIS Inc |
Kasyanova E.,IIS Inc
Scientific Visualization | Year: 2014
Visualization is a process of transformation of large and complex abstract forms of information into visual form, strengthening user's cognitive abilities and allowing them to take the most optimal decisions. Graphs are the most common abstract structure encountered in computer science and are widely used for abstract information representation. Any system that consists of discrete states (or sites) and connections between them can be modeled by a graph. Methods and tools for graph visualization are widely used in many applications, such as system and applied programming, biology, chemistry, artificial intellect, analysis of financial information, sociology and many others. In the paper, a survey on main existent methods of information visualization on the base of graph models is given.
News Article | November 3, 2016
NEW YORK, Nov. 3, 2016 /PRNewswire/ -- IIS announced they have been named HPE 2016 Global Partner of the Year. In response to this recognition they have released the following statement: There was no shortage of competition. With a large network of HPE partners across the world,...