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Okazaki Y.,Fuji Womens University | Utama Z.,Hiroshima University | Suidasari S.,Hiroshima University | Zhang P.,Hiroshima University | And 4 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2012

To examine the effect of supplemental dietary vitamin B6 on the colonic luminal environment, growing male rats were fed a high-fat diet containing 1, 7, or 35 mg pyridoxine HCl/kg diet for 6 wk. Food intake and growth were unaffected by the dietary treatment. Supplemental dietary vitamin B6 signifi cantly reduced the production of a fecal secondary bile acid, lithocholic acid (the most toxic secondary bile acid and a risk factor for colon cancer), and markedly reduced the ratio of lithocholic acid to deoxycholic acid (a less toxic secondary bile acid) in feces (p<0.05). Increasing dietary vitamin B6 increased fecal mucin levels (a marker of intestinal barrier function) in a dose-dependent manner (p<0.05) but did not affect fecal immunoglobulin A levels (an index of intestinal immune function). Cecal levels of organic acids were not signifi cantly affected by supplemental dietary vitamin B6. These results suggest the possibility that dietary vitamin B6 affects the colonic luminal environment by altering the production of secondary bile acids and mucins. Source

Hosaka T.,Tokushima University | Nii Y.,Tokushima Prefectural Industrial Technology Center | Tomotake H.,Iida Womens Junior College | Ito T.,Tokushima University | And 9 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2012

Buckwheat has been shown to have various health benefits such as reduction of hypertension and improvement of hypercholesterolemia; however, its effect on diabetes has not been fully elucidated. In this study, buckwheat bran extracts (BBE) inhibited sucrase activity in vitro more effectively than buckwheat. Balb/c mice pretreated with BBE showed dose-dependent reductions of blood glucose, greater than those observed with control mice, within 60 min following oral sucrose administration. Blood glucose levels in mice pretreated with buckwheat extracts were also significantly lower compared to those in control mice within 30 min following oral administration of sucrose. However, rutin, one of the abundant polyphenols of BBE, did not lower blood glucose level. Our data indicate that components of BBE other than rutin have inhibitory activity against sucrase in vivo. These results suggest that BBE could have beneficial effects on diabetes. Source

Utama Z.,Hiroshima University | Okazaki Y.,Fuji Womens University | Tomotake H.,Iida Womens Junior College | Kato N.,Hiroshima University
Plant Foods for Human Nutrition | Year: 2013

The present study investigated the effect of dietary tempe, a fermented soy product, on the colonic environment of rats fed high-fat (HF, 30 % fat; experiment 1) or low-fat (LF, 6 % fat; experiment 2) diets. Growing male rats were fed the experimental diets with or without 25 % tempe for 21 days. Tempe consumption slightly but significantly increased the growth of rats fed both the HF and LF diets (P < 0.05). With both the HF and LF diets, dietary tempe markedly reduced a harmful fecal secondary bile acid, lithocholic acid (a risk factor of colon cancer) (P < 0.05), and markedly elevated fecal mucins (indices of intestinal barrier function) and immunoglobulin A (IgA, an index of intestinal immune function) (P < 0.05). With the HF diet, dietary tempe increased cecal acetate, butyrate, propionate, and succinate concentrations (P < 0.05). Analysis of the profile of cecal microflora revealed lower Bacteroides and higher Clostridium cluster XIVa levels in the tempe group of rats fed the HF diet (P < 0.05). Compared with the control group, the fecal activity of β-glucosidase was markedly higher in the tempe group (P < 0.05), while that of urease was lower (P < 0.05) with both the HF and LF diets. The present results suggest that tempe consumption modulates the colonic environment in rats. © 2013 Springer Science+Business Media New York. Source

Okazaki Y.,Fuji Womens University | Tomotake H.,Iida Womens Junior College | Tsujimoto K.,Seiren Co. | Sasaki M.,Seiren Co. | Kato N.,Hiroshima University
Journal of Nutrition | Year: 2011

We previously reported that consumption of a resistant protein, sericin, reduces colon tumorigenesis, constipation, and serum TG in rodents. The present study was conducted to elucidate the effects of dietary sericin on the intestinal luminal environment in rats fed a high-fat (HF) diet. Rats were fed 300 or 50 g/kg of beef tallow with or without 40 g/kg sericin, a protein purified from cocoons of Bombix mori, for 3 wk. Intestinal luminal variables, including IgA (index of intestinal immune function), mucins (index of barrier function), organic acids, microflora, and secondary bile acids, were measured. Dietary sericin markedly elevated fecal IgA in the HF diet group (3-fold, P < 0.05) but not in the low-fat (LF) diet group. Fecal mucin levels were elevated by sericin intake in the HF diet group (P < 0.05). Cecal organic acids, including acetate, propionate, n-butyrate, and succinate, were significantly lower in the HF diet group compared with the LF diet group. Dietary sericin significantly elevated cecal acetate and n-butyrate in the HF diet group but not in the LF diet group. Compared with the LF diet, the HF diet significantly increased serum TG in the untreated group but not in those fed sericin. The HF diet increased lower density lipoprotein (VLDL + IDL + LDL) cholesterol and it was reduced by sericin intake (P < 0.05). There was an inverse correlation between serum TG and cecal acetate (Spearman rank correlation coefficient = 20.63; P < 0.001). The profile of microflora in cecal digesta and fecal secondary bile acids (a risk factor for colon cancer) did not differ between the HF diet and HF diet with sericin groups. These results suggest a novel and favorable effect of sericin on colon health by modulating intestinal immune and barrier functions and fermentation in rats fed a HF diet. © 2011 American Society for Nutrition. Source

Hosaka T.,Tokushima University | Hosaka T.,Kyorin University | Sasaga S.,Tokushima University | Yamasaka Y.,Tokushima University | And 8 more authors.
Journal of Medical Investigation | Year: 2014

Buckwheat powder or protein has been shown to decrease the total serum cholesterol level in non-diabetic mice or rats. However, the lipid-lowering effect of buckwheat bran extract (BBE) in diabetic mice has not been fully elucidated. KK-Ay mice that received six-week treatment with BBE showed decreased body weight and liver weight compared to those of control (vehicle) mice. However, there was no significant difference in food intake. BBE treatments prevented liver triglyceride accumulation and decreased the serum level of triglycerides. In addition, mRNA expression levels lipogenic enzyme genes, fatty acid synthase, acetyl-coenzyme a oxidase and stearyl-coenzyme a desaturase 1, but not those of β-oxidized enzyme genes, were decreased in BBE-treated mice. Level of transcription factors ChREBP and SREBP1c, transcripts of lipogenic genes, were also decreased in BBE-treated mice. These results suggest that chronic treatment with BBE derivatives could have beneficial effects on hypertriglycemia in patients with type 2 diabetes mellitus. © 2014 by The University of Tokushima Faculty of Medicine. Source

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