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Lora J.,IHSM La Mayora CSIC UMA | Hormaza J.I.,IHSM La Mayora CSIC UMA | Herrero M.,CSIC - Aula Dei Experimental Station
Acta Horticulturae | Year: 2012

Cherimoya (Annona cherimola Mill.) is a subtropical fruit tree with an incipient but promising commercial production niche in subtropical climates. However, the expansion of this crop from its areas of origin has taken place without its natural pollinating agent and crop production relies heavily on hand pollination. Erratic yields are often obtained without apparent causes, and they could be related to the lack of knowledge on the biological requirements of pollen in this species. Since pollen performance in the pistil is dependent on previous pollen development in the anther, here we perform a study on the influence of temperature and water content on final pollen development stages and, as a consequence, on pollen performance in A. cherimola. The results show a significant influence of temperature and relative humidity on pollen performance. These results provide biological support for adequate pollen handling to optimize production in this species.

Encina C.L.,IHSM La Mayora CSIC UMA | Martin E.C.,IHSM La Mayora CSIC UMA | Lopez A.A.,IHSM La Mayora CSIC UMA | Padilla I.M.G.,Centro IFAPA Churriana
Revista Brasileira de Fruticultura | Year: 2014

Annonaceae is an ancient family of plants including approximately 50 genera growing worldwide in a quite restricted area with specific agroclimatic requirements. Only few species of this family has been cultivated and exploited commercially and most of them belonging to the genus Annona such as A. muricata, A. squamosa, the hybrid A. cherimola x A. squamosa and specially Annona cherimola: the cherimoya, commercially cultivated in Spain, Chile, California, Florida, México, Australia, Ecuador, Peru, Brazil, New Zealand and several countries in South and Central America. The cherimoya shows a high degree of heterozygosis, and to obtain homogeneous and productive orchards it is necessary to avoid the propagation by seeds of this species. Additionally, the traditional methods of vegetative propagation were inefficient and inadequate, due to the low morphogenetic potential of this species, and the low rooting rate. The in vitro tissue culture methods of micropropagation can be applied successfully to cherimoya and other Annona sp to overcome these problems. Most of the protocols of micropropagation and regeneration were developed using the cultivar Fino de Jete, which is the major cultivar in Spain. First it is developed the method to micropropagate the juvenile material of cherimoya (ENCINA et al., 1994), and later it was optimized a protocol to micropropagate adult cherimoya genotypes selected by outstanding agronomical traits (PADILLA and ENCINA, 2004) and further it was improved the process through micrografting (PADILLA and ENCINA, 2011).At the present time we are involved in inducing and obtaining new elite genotypes, as part of a breeding program for the cherimoya and other Annonas, using and optimizing different methodologies in vitro: a) Adventitious organogenesis and regeneration from cellular cultures (ENCINA, 2004), b) Ploidy manipulation of the cherimoya, to obtain haploid, tetraploid and triploid plants (seedless), c) Genetic transformation, for the genes introduction to control the postharvest processes and the genes introduction to provide resistance to pathogen and insects and d) Micropropagation and regeneration of other wild Annona or related Annonaceae species such as: Annona senegalensis, A. scleroderma, A. montana, A. reticulata, A. glabra, A. diversifolia and Rollinia sp.

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