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Adeje, Spain

Martin C.,IHSM la Mayora CSIC | Herrero M.,EE Aula Dei | Hormaza J.I.,IHSM la Mayora CSIC
PLoS ONE | Year: 2011

Increasing germplasm erosion requires the recovery and conservation of traditional cultivars before they disappear. Here we present a particular case in Spain where a thorough prospection of local fruit tree species was performed in the 1950s with detailed data of the origin of each genotype but, unfortunately, the accessions are no longer conserved in ex situ germplasm collections. However, for most of those cultivars, an old stone collection is still preserved. In order to analyze the diversity present at the time when the prospection was made and to which extent variability has been eroded, we developed a protocol in apricot (Prunus armeniaca L.) to obtain DNA from maternal tissues of the stones of a sufficient quality to be amplified by PCR. The results obtained have been compared with the results from the profiles developed from apricot cultivars currently conserved in ex situ germplasm collections. The results highlight the fact that most of the old accessions are not conserved ex situ but provide a tool to prioritize the recovery of particular cultivars. The approach used in this work can also be applied to other plant species where seeds have been preserved. © 2011 Martín et al. Source

Alcaraz M.L.,IHSM la Mayora CSIC | Hormaza J.I.,IHSM la Mayora CSIC
Acta Horticulturae | Year: 2012

In avocado, only a very small fraction of flowers produced at anthesis are able to set fruits due to a massive drop of flowers and developing fruitlets. To explore the implications of the nutritive status of the flower in the reproductive process, in this work starch content in avocado pistils has been examined in pollinated and nonpollinated flowers at anthesis and during the days following anthesis. Each flower was histochemically processed to be observed under the microscope after staining with I2KI for starch reserves. Starch content in the stained preparations was measured with the help of an image analysis system attached to the microscope. Flowers at anthesis were rich in starch, which was widely compartmentalized into the different pistil tissues. While no external differences could be recorded among flowers at anthesis, a wide variability could be observed in starch content. Pistil weight increased in the days following anthesis in both pollinated and non-pollinated flowers, and this initial development appears to be independent of pollination. Source

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