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Bellaria-Igea Marina, Italy

Meneghelli M.,University of Padua | Pasinato A.,University of Padua | Salvadori S.,University of Padua | Gaio P.,University of Padua | And 4 more authors.
Journal of Perinatology | Year: 2016

Objective:The objective of this study was to evaluate possible influences of parenteral nutrition on growth and bone development in preterms and to search for markers of bone status.Study Design:Metacarpus bone transmission time (mc-BTT) was performed at birth, 21 days and 36 weeks of gestational age (GA) in preterms, receiving two different nutritional regimens, together with biochemical analysis.Result:A total of 234 patients were studied. Newborns with aggressive nutrition had significantly better growth rate and higher values of mc-BTT until discharge. Mc-BTT at day 21 correlates positively with nutritional intakes and phosphatemia; lower limb length positively correlated with mc-BTT (P<0.01). Newborns with low energy intake in the first week of life (<70 kcal kg-1 per day) and low serum phosphate level (<1.4 mmol l-1) at 21 days had lower mc-BTT at 36 weeks of GA (P<0.01).Conclusion:Aggressive parenteral intakes in preterms improve growth and bone status in the short-medium term, suggesting that early nutrition could influence bone development. © 2016 Nature America, Inc. All rights reserved. Source


Ongaro A.,University of Ferrara | Pellati A.,University of Ferrara | Bagheri L.,University of Ferrara | Fortini C.,University of Ferrara | And 2 more authors.
Bioelectromagnetics | Year: 2014

Pulsed electromagnetic fields (PEMFs) play a regulatory role on osteoblast activity and are clinically beneficial during fracture healing. Human mesenchymal stem cells (MSCs) derived from different sources have been extensively used in bone tissue engineering. Compared with MSCs isolated from bone marrow (BMSCs), those derived from adipose tissue (ASCs) are easier to obtain and available in larger amounts, although they show a less osteogenic differentiation potential than BMSCs. The hypothesis tested in this study was to evaluate whether PEMFs favor osteogenic differentiation both in BMSCs and in ASCs and to compare the role of PEMFs alone and in combination with the biochemical osteogenic stimulus bone morphogenetic protein (BMP)-2. Early and later osteogenic markers, such as alkaline phosphatase (ALP) activity, osteocalcin levels, and matrix mineralization, were analyzed at different times during osteogenic differentiation. Results showed that PEMFs induced osteogenic differentiation by increasing ALP activity, osteocalcin, and matrix mineralization in both BMSCs and ASCs, suggesting that PEMF activity is maintained during the whole differentiation period. The addition of BMP-2 in PEMF exposed cultures further increased all the osteogenic markers in BMSCs, while in ASCs, the stimulatory role of PEMFs was independent of BMP-2. Our results indicate that PEMFs may stimulate an early osteogenic induction in both BMSCs and ASCs and they suggest PEMFs as a bioactive factor to enhance the osteogenesis of ASCs, which are an attractive cell source for clinical applications. In conclusion, PEMFs may be considered a possible tool to improve autologous cell-based regeneration of bone defects in orthopedics. © 2014 Wiley Periodicals, Inc. Source


Trademark
Igea Inc | Date: 2008-08-19

Medical and hospital electromedical equipment for electroporation, electrochemotherapy, tumor tissue ablation and gene therapy, namely, an electroporator that uses high-voltage pulses.


Igea Inc | Entity website

Gennaio 2015 L'elettrochemioterapia rimborsata dal Sistema Sanitario Svizzero L'elettrochemioterapia stata inserita nell'elenco degli interventi chirurgici rimborsati dallo Sviss DRG system. In Svizzera gi tre centri oncologici utilizzano l'elettrochemioterapia applicata con Cliniporator ...


Ongaro A.,University of Ferrara | Varani K.,University of Ferrara | Masieri F.F.,University of Ferrara | Pellati A.,University of Ferrara | And 7 more authors.
Journal of Cellular Physiology | Year: 2012

Synovial fibroblasts (SFs) contribute to the development of osteoarthritis (OA) by the secretion of a wide range of pro-inflammatory mediators, including cytokines and lipid mediators of inflammation. Previous studies suggest that electromagnetic fields (EMFs) may represent a potential therapeutic approach to limit cartilage degradation and control inflammation associated to OA, and that they may act through the adenosine pathway. Therefore, we investigated whether EMFs might modulate inflammatory activities of human SFs from OA patients (OASFs) treated with interleukin-1β (IL-1β), and the possible involvement of adenosine receptors (ARs) in mediating EMF effects. EMF exposure induced a selective increase in A 2A and A 3 ARs. These increases were associated to changes in cAMP levels, indicating that ARs were functionally active also in EMF-exposed cells. Functional data obtained in the presence of selective A 2A and A 3 adenosine agonists and antagonists showed that EMFs inhibit the release of prostaglandin E 2 (PGE 2) and the proinflammatory cytokines interleukin-6 (IL-6) and interleukin-8 (IL-8), while stimulating the release of interleukin-10 (IL-10), an antinflammatory cytokine. These effects seem to be mediated by the EMF-induced upregulation of A 2A and A 3 ARs. No effects of EMFs or ARs have been observed on matrix degrading enzyme production. In conclusion, this study shows that EMFs display anti-inflammatory effects in human OASFs, and that these EMF-induced effects are in part mediated by the adenosine pathway, specifically by the A 2A and A 3 AR activation. Taken together, these results open new clinical perspectives to the control of inflammation associated to joint diseases. © 2011 Wiley Periodicals, Inc. Source

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