IFAPA Rancho de la Merced

Jerez de la Frontera, Spain

IFAPA Rancho de la Merced

Jerez de la Frontera, Spain
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Pineiro Z.,IFAPA Rancho de la Merced | Marrufo-Curtido A.,IFAPA Rancho de la Merced | Vela C.,IFAPA Rancho de la Merced | Palma M.,University of Cádiz
Food and Bioproducts Processing | Year: 2017

By-products from the winemaking companies, specifically woody vine material, can be an interesting source of bioproducts, specifically stilbenes and related compounds, however the extraction conditions can dramatically modify the final recoveries of these beneficial compounds. The effects of microwave-assisted extraction on the recovery of stilbenes have been evaluated in this manuscript. The stability under the extraction conditions was assessed in order to define the working range. A fractional factorial experimental design was developed to analyse the influence on the extraction process of seven different extraction variables: solvent, stirring, extraction temperature, extraction time, microwave power, sample weight and extraction volume. This new method enables stilbenes to be extracted from grape stems and canes in only 5 min, with an extraction temperature of 125 °C and 80% ethanol in water as the extraction solvent. Repeatability and reproducibility were also evaluated and the resulting RSD values (n = 5) were lower than 10% for all analysed stilbenes. Additionally, stilbenes in 20 different grape stem and cane samples were determined. © 2017 Institution of Chemical Engineers


Cantos M.,CSIC - Institute of Natural Resources and Agriculture Biology of Seville | Arroyo-Garcia R.,Technical University of Madrid | Garcia J.L.,CSIC - Institute of Natural Resources and Agriculture Biology of Seville | Lara M.,IFAPA Rancho de la Merced | And 10 more authors.
Comptes Rendus - Biologies | Year: 2017

For decades, human activities have gradually destroyed the natural habitats of wild grapevine, Vitis vinifera L. subsp. sylvestris (Gmelin) Hegi, and nowadays this species is endangered in southern Europe. In this paper, 94 populations of this species have been localized and characterized in the Andalusian region in the Iberian Peninsula between 1989 and 2013. Location, ecological aspects, and sanitary characteristics are described. Must properties and in vitro tolerance to calcareous conditions were also checked. The paper also contains a global description of female and male individuals. Two hundred individuals from six river basin populations have been sampled, and their genetic structure analyzed by using 25 nuclear microsatellites loci to investigate the gene diversity of wild grape populations in Andalusia at two levels: total individuals and at river basin populations. Also, the genetic relationship of wild and cultivated accessions has been tested. Wild grapevine is considered the ancestor of the cultivated varieties and should be preserved as this material could be used to start breeding programs of cultivated varieties and also to restore riverbank forests, which constitute one of the worst preserved ecosystems in the area. © 2017 Académie des sciences


Arroyo-Garcia R.,Technical University of Madrid | Cantos M.,CSIC - Institute of Natural Resources and Agriculture Biology of Seville | Lara M.,IFAPA Rancho de la Merced | Lopez M.A.,University of Seville | And 6 more authors.
Spanish Journal of Agricultural Research | Year: 2016

Wild grapevine is becoming a threatened species in the Iberian Peninsula due to human impacts. The aim of this work was to carry out a holistic study for six years of the largest wild grapevine population found up to date in SW Iberian Peninsula. This population has 115 vines. Ampelographic and soil characteristics have been studied. Evaluation of its environment has also been studied by describing the main parasitic species and natural enemies of pests. The ability of this plant material for its micropropagation and storage in slow-growth conditions has been tested. Microvinification resulted in a wine with good acidity and medium color intensity, two interesting characteristics under a warm climatology. Finally, the identification of private alleles in this wild population, absent in other locations from the Northern and Southern Iberian territories, is a very valuable feature and confirms the importance of establishing conservation programs. The population here studied is genetically unique and potentially useful for commercial rootstocks and cultivars breeding that would improve viticulture and enology. © 2016 INIA.


Pineiro Z.,IFAPA Rancho de la Merced | Guerrero R.F.,IFAPA Rancho de la Merced | Fernandez-Marin M.I.,IFAPA Rancho de la Merced | Cantos-Villar E.,IFAPA Rancho de la Merced | Palma M.,University of Cádiz
Journal of Agricultural and Food Chemistry | Year: 2013

A new method for fast determination of stilbenoids from grape stems was developed. Ultrasound-assisted extraction was applied prior to chromatographic determination of stilbenoids in the extracts, and the stability of stilbenoids under extraction conditions was checked. A fractional experimental design was developed to analyze the influence on the extraction process of seven different extraction variables: temperature, ultrasound amplitude, ultrasonic cycle duration, ultrasonic probe type, time, sample-solvent ratio, and solvent (mixtures of ethanol and water). The most important variables for the recovery of major stilbenoids were studied and the final conditions optimized. With this new method, the main stilbenoids found in grape stems can be extracted in 15 min, using 75 C as the extraction temperature and 80% ethanol as the extraction solvent, and no cleaning step with organic solvent is needed. The optimized method allowed for the analysis of stilbenoid content from 22 grape stem samples, many of them analyzed for the first time. © 2013 American Chemical Society.


Pineiro Z.,IFAPA Rancho de la Merced | Cantos-Villar E.,IFAPA Rancho de la Merced | Palma M.,University of Cádiz | Puertas B.,IFAPA Rancho de la Merced
Journal of Agricultural and Food Chemistry | Year: 2011

A validated HPLC method with fluorescence detection for the simultaneous quantification of hydroxytyrosol and tyrosol in red wines is described. Detection conditions for both compounds were optimized (excitation at 279 and 278 and emission at 631 and 598 nm for hydroxytyrosol and tyrosol, respectively). The validation of the analytical method was based on selectivity, linearity, robustness, detection and quantification limits, repeatability, and recovery. The detection and quantification limits in red wines were set at 0.023 and 0.076 mg L -1 for hydroxytyrosol and at 0.007 and 0.024 mg L -1 for tyrosol determination, respectively. Precision values, both within-day and between-day (n = 5), remained below 3% for both compounds. In addition, a fractional factorial experimental design was developed to analyze the influence of six different conditions on analysis. The final optimized HPLC-fluorescence method allowed the analysis of 30 nonpretreated Spanish red wines to evaluate their hydroxytyrosol and tyrosol contents. © 2011 American Chemical Society.


Pineiro Z.,IFAPA Rancho de la Merced | Marrufo-Curtido A.,IFAPA Rancho de la Merced | Serrano M.J.,IFAPA Rancho de la Merced | Palma M.,University of Cádiz
Molecules | Year: 2016

An analytical ultrasound-assisted extraction (UAE) method has been optimized and validated for the rapid extraction of stilbenes from grape canes. The influence of sample pre-treatment (oven or freeze-drying) and several extraction variables (solvent, sample-solvent ratio and extraction time between others) on the extraction process were analyzed. The new method allowed the main stilbenes in grape canes to be extracted in just 10 min, with an extraction temperature of 75°C and 60% ethanol in water as the extraction solvent. Validation of the extraction method was based on analytical properties. The resulting RSDs (n = 5) for interday/intraday precision were less than 10%. Furthermore, the method was successfully applied in the analysis of 20 different grape cane samples. The result showed that grape cane byproducts are potentially sources of bioactive compounds of interest for pharmaceutical and food industries. © 2016 by the authors; licensee MDPI.


PubMed | University of Cádiz and IFAPA Rancho de la Merced
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016

An analytical ultrasound-assisted extraction (UAE) method has been optimized and validated for the rapid extraction of stilbenes from grape canes. The influence of sample pre-treatment (oven or freeze-drying) and several extraction variables (solvent, sample-solvent ratio and extraction time between others) on the extraction process were analyzed. The new method allowed the main stilbenes in grape canes to be extracted in just 10 min, with an extraction temperature of 75 C and 60% ethanol in water as the extraction solvent. Validation of the extraction method was based on analytical properties. The resulting RSDs (n = 5) for interday/intraday precision were less than 10%. Furthermore, the method was successfully applied in the analysis of 20 different grape cane samples. The result showed that grape cane byproducts are potentially sources of bioactive compounds of interest for pharmaceutical and food industries.


PubMed | IFAPA Rancho de la Merced
Type: Evaluation Studies | Journal: Journal of agricultural and food chemistry | Year: 2011

A validated HPLC method with fluorescence detection for the simultaneous quantification of hydroxytyrosol and tyrosol in red wines is described. Detection conditions for both compounds were optimized (excitation at 279 and 278 and emission at 631 and 598 nm for hydroxytyrosol and tyrosol, respectively). The validation of the analytical method was based on selectivity, linearity, robustness, detection and quantification limits, repeatability, and recovery. The detection and quantification limits in red wines were set at 0.023 and 0.076 mg L(-1) for hydroxytyrosol and at 0.007 and 0.024 mg L(-1) for tyrosol determination, respectively. Precision values, both within-day and between-day (n = 5), remained below 3% for both compounds. In addition, a fractional factorial experimental design was developed to analyze the influence of six different conditions on analysis. The final optimized HPLC-fluorescence method allowed the analysis of 30 nonpretreated Spanish red wines to evaluate their hydroxytyrosol and tyrosol contents.


PubMed | IFAPA Rancho de la Merced
Type: Evaluation Studies | Journal: Journal of agricultural and food chemistry | Year: 2013

A new method for fast determination of stilbenoids from grape stems was developed. Ultrasound-assisted extraction was applied prior to chromatographic determination of stilbenoids in the extracts, and the stability of stilbenoids under extraction conditions was checked. A fractional experimental design was developed to analyze the influence on the extraction process of seven different extraction variables: temperature, ultrasound amplitude, ultrasonic cycle duration, ultrasonic probe type, time, sample-solvent ratio, and solvent (mixtures of ethanol and water). The most important variables for the recovery of major stilbenoids were studied and the final conditions optimized. With this new method, the main stilbenoids found in grape stems can be extracted in 15 min, using 75 C as the extraction temperature and 80% ethanol as the extraction solvent, and no cleaning step with organic solvent is needed. The optimized method allowed for the analysis of stilbenoid content from 22 grape stem samples, many of them analyzed for the first time.

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