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Belaj A.,IFAPA CentroAlameda del Obispo | Dominguez-Garcia M.d.C.,IFAPA CentroAlameda del Obispo | Atienza S.G.,CSIC - Institute for Sustainable Agriculture | Martin Urdiroz N.,University of Cordoba, Spain | And 7 more authors.
Tree Genetics and Genomes | Year: 2012

Molecular markers (SSR, SNP and DArT) and agronomical traits have been used in the world's largest olive (Olea europaea L.) germplasm collection (IFAPA, Centre Alameda del Obispo, Cordoba, Spain) to study the patterns of genetic diversity and underlying genetic structure among 361 olive accessions. In addition the marker data were used to construct a set of core collections by means of two different algorithms (MSTRAT and PowerCore) based on M (maximization) strategy. Our results confirm that the germplasm collection is a useful source of genetically diverse material. We also found that geographical origin is an important factor structuring genetic diversity in olive. Subsets of 18, 27, 36, 45 and 68 olive accessions, representing respectively 5%, 7. 5%, 10%, 12. 5% and 19% of the whole germplasm collection, were selected based on the information obtained by all the data set as well as each marker type considered individually. According to our results, the core collections that represent between 19% and 10% of the total collection size could be considered as optimal to retain the bulk of the genetic diversity found in this collection. Due to its high efficiency at capturing all the alleles/traits states found in the whole collection, the core size of 68 accessions could be of special interest for genetic conservation applications in olive. The high average genetic distance and diversity and the almost equal representation of accessions from different geographical regions indicate that the core size of 36 accessions, could be the working collection for olive breeders. © 2011 Springer-Verlag.

Noormohammadi Z.,Islamic Azad University at Tehran | Trujillo I.,University of Cordoba, Spain | Belaj A.,IFAPA CentroAlameda del Obispo | Ataei S.,National Institutes of Genetic Engineering and Biotechnology NIGEB | Hosseini-Mazinan M.,National Institutes of Genetic Engineering and Biotechnology NIGEB
Scientia Horticulturae | Year: 2014

A collection of 52 local Iranian olive genotypes (North and south of Iran) and 36 olive cultivars from Mediterranean countries were studied in order to determine the genetic structure and their relationships. Thirteen primer pairs of microsatellite loci were selected that were known to be discriminating from previous study on olive. Polymorphic Information Content values ranged from 0.51 to 0.95. The Gst analysis for SSR loci revealed that some of the loci are more common while, some other loci are present in certain populations and show limited distribution among populations. Analysis of molecular variance (AMOVA) revealed that 11.71% of genetic variation attributed to differences between geographical regions while 88.29% came from variation in within regions (P<. 0.05). Pairwise Fst test revealed significant differences (P< 0.05) between populations except between West and East Mediterranean populations. Neighbor Joining cluster analysis grouped the olive cultivars in two distinct clusters separating, olive genotypes located in North of Iran from the rest of olive cultivars studied. STRUCTURE and K means analyses showed the presence of distinct groups in olive trees and revealed variability in allelic compositions and their frequency between Iranian and West and East Mediterranean olive cultivars. The results of Bayesian clearly revealed that due to frequent occurrence of gene flow, the West and East Mediterranean olive cultivars had lower Fst values compared to the other population groups. In present study, genetic structure revealed admixture allelic compositions among Mediterranean olive cultivars different from Iran genotypes. © 2014 Elsevier B.V.

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