Neto A.I.,University of Minho |
Neto A.I.,Icvs 3Bspt Government Assoc Laboratory |
Correia C.R.,University of Minho |
Correia C.R.,Icvs 3Bspt Government Assoc Laboratory |
And 8 more authors.
We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering. © The Royal Society of Chemistry 2015. Source