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Lorenzi A.T.,Molecular Oncology Center | Fregnani J.H.T.G.,Center for the Researcher Support | Possati-Resende J.C.,Barretos Cancer Hospital | Neto C.S.,Barretos Cancer Hospital | And 6 more authors.
Gynecologic Oncology | Year: 2013

Objective. Cervical cancer is the second most common cancer among Brazilian women. High-risk human papillomavirus (hr-HPV) persistence is the primary cause of cervical neoplasia. Early detection of hr-HPV is important for identifying women at risk for developing cervical lesions. Approximately 85% of new cases of cervical cancer worldwide and 50% of the total cervical cancer deaths occurred in developing countries. Here, a newmethodology to support a cervical cancer screening program was evaluated in women from various Brazilian regions. Methods. Two thousand women aged 18-77 years were enrolled in an opportunistic cervical cancer screening programandwere randomized into self-vaginal or health professional-guided cervical sampling groups. The Qiagen careHPV™testwas performed on all samples. Pap testswere performed on all women using liquid-based cytology. Results. Positive hr-HPV resultswere obtained in 12.3% (245/2000) ofwomen; similar rateswere observed in self- or health professional-collected samples. Eighty-nine percent (1719/2000) of cervical cytologies classified as normal were negative to hr-HPV. Among the cytological samples, 36.6% classified as ASC-US+ were positive to hr-HPV, 78.8% were LSIL and 75.0% were HSIL. Conclusions. Self-sampled and health professional-sampled vaginal/cervical specimens did not differ in their rates of detection of hr-HPV. Therefore, HPV DNA testing in self-sampled vaginal cells is an alternative to primary screening in low-resource settings. © 2013 Elsevier Inc. All rights reserved. Source


Gomes A.P.,University of Beira Interior | Mano J.F.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Mano J.F.,Icvs 3Bs Pt Government Assoc Laboratory | Queiroz J.A.,University of Beira Interior | Gouveia I.C.,University of Beira Interior
Carbohydrate Polymers | Year: 2015

A large group of low molecular weight natural compounds that exhibit antimicrobial activity has been isolated from animals and plants during the past two decades. Among them, peptides are the most widespread resulting in a new generation of antimicrobial agents with higher specific activity. In the present study we have developed a new strategy to obtain antimicrobial wound-dressings based on the incorporation of antimicrobial peptides into polyelectrolyte multilayer films built by the alternate deposition of polycation (chitosan) and polyanion (alginic acid sodium salt) over cotton gauzes. Energy dispersive X ray microanalysis technique was used to determine if antimicrobial peptides penetrated within the films. FTIR analysis was performed to assess the chemical linkages, and antimicrobial assays were performed with two strains: Staphylococcus aureus (Gram-positive bacterium) and Klebsiella pneumonia (Gram-negative bacterium). Results showed that all antimicrobial peptides used in this work have provided a higher antimicrobial effect (in the range of 4 log-6 log reduction) for both microorganisms, in comparison with the controls, and are non-cytotoxic to normal human dermal fibroblasts at the concentrations tested. © 2015 Elsevier Ltd. Source


Barbosa S.,University of Santiago de Compostela | Topete A.,University of Santiago de Compostela | Alatorre-Meda M.,University of Santiago de Compostela | Alatorre-Meda M.,Icvs 3Bs Pt Government Assoc Laboratory | And 6 more authors.
Journal of Physical Chemistry C | Year: 2014

This paper reports the development of a multimodal therapy nanoplatform based on gold nanostars (Au NS) as core particles. These NS were functionalized with the chemotherapeutic drug doxorubicin (DOXO), which was conjugated to the NS surface by means of a cleavable heterobifunctional cross-linker (sulfo-LC-SPDP) to allow its release under the action of reducing enzymes. To ensure a specific delivery of the chemotherapeutic drug, the nanoplatform was additionally functionalized with folic acid (FA) as targeting ligand and cellular uptake adjuvant. By synthetically modifying the plasmon band of Au NS to the near-infrared (NIR) region of the electromagnetic spectrum, the present nanoplatform was able to simultaneously combine the capability of photothermal therapy (PTT) through the conversion of absorbed light energy into localized heat and chemotherapy, enabling their monitoring by means of optical fluorescence imaging thanks to DOXOs autofluorescence. Cellular uptake was observed to be enhanced when the Au NPs were decorated with the targeting ligand. In addition, the therapeutic efficiency of the nanoplatform tested in HeLa cells demonstrated the larger cytotoxicity efficiency of the combined therapy if compared to individual ones. © 2014 American Chemical Society. Source


Lima A.C.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Lima A.C.,Icvs 3Bs Pt Government Assoc Laboratory | Mano J.F.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Mano J.F.,Icvs 3Bs Pt Government Assoc Laboratory
Nanomedicine | Year: 2015

Inspired by natural structures, great attention has been devoted to the study and development of surfaces with extreme wettable properties. The meticulous study of natural systems revealed that the micro/nano-topography of the surface is critical to obtaining unique wettability features, including superhydrophobicity. However, the surface chemistry also has an important role in such surface characteristics. As the interaction of biomaterials with the biological milieu occurs at the surface of the materials, it is expected that synthetic substrates with extreme and controllable wettability ranging from superhydrophilic to superhydrophobic regimes could bring about the possibility of new investigations of cell-material interactions on nonconventional surfaces and the development of alternative devices with biomedical utility. This first part of the review will describe in detail how proteins and cells interact with micro/nano-structured surfaces exhibiting extreme wettabilities. © 2015 Future Medicine Ltd. Source


Pinto F.,University of Minho | Pinto F.,Icvs 3Bs Pt Government Assoc Laboratory | Pertega-Gomes N.,University of Minho | Pertega-Gomes N.,Icvs 3Bs Pt Government Assoc Laboratory | And 13 more authors.
Clinical Cancer Research | Year: 2014

Purpose: Successful therapy of patients with prostate cancer is highly dependent on reliable diagnostic and prognostic biomarkers. Brachyury is considered a negative prognostic factor in colon and lung cancer; however, there are no reports on Brachyury's expression in prostate cancer.Experimental Design: In this study, we aimed to assess the impact of Brachyury expression in prostate tumorigenesis using a large series of human prostate samples comprising benign tissue, prostate intraepithelial neoplasia (PIN) lesions, localized tumor, and metastatic tissues. The results obtained were compared with what can be inferred from the Oncomine database. In addition, multiple in vitro models of prostate cancer were used to dissect the biologic role of Brachyury in prostate cancer progression.Results: We found that Brachyury is significantly overexpressed in prostate cancer and metastatic tumors when compared with normal tissues, both at protein and at mRNA levels. Brachyury expression in the cytoplasm correlates with highly aggressive tumors, whereas the presence of Brachyury in the nucleus is correlated with tumor invasion. We found that Brachyury-positive cells present higher viability, proliferation, migration, and invasion rates than Brachyury-negative cells. Microarray analysis further showed that genes co-expressed with Brachyury are clustered in oncogenic-related pathways, namely cell motility, cellcycle regulation, and cell metabolism.Conclusions: Collectively, the present study suggests that Brachyury plays an important role in prostate cancer aggressiveness and points, for the first time, to Brachyury as a significant predictor of poor prostate cancer prognosis. Our work paves the way for future studies assessing Brachyury as a possible prostate cancer therapeutic target. © 2014 American Association for Cancer Research. Source

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