Icia Instituto Canario Of Investigaciones Agrarias

Santa Cruz de Tenerife, Spain

Icia Instituto Canario Of Investigaciones Agrarias

Santa Cruz de Tenerife, Spain

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Rivera D.,University of Murcia | Obon C.,University Miguel Hernández | Alcaraz F.,University of Murcia | Egea T.,University Miguel Hernández | And 4 more authors.
Taxon | Year: 2013

The nomenclature of the Canary Island endemic palm, Phoenix canariensis, is reviewed. It is concluded that Hermann Wildpret is the correct author of Phoenix canariensis. Phoenix canariensis, P. cycadifolia Regel and P. jubae (Webb & Berthel.) Webb ex H. Christ. are lectotypified.


Lerias J.R.,University of Lisbon | Lerias J.R.,IBET Institute Biologia Experimental e Tecnologica | Hernandez-Castellano L.E.,University of Las Palmas de Gran Canaria | Morales-delaNuez A.,University of Las Palmas de Gran Canaria | And 9 more authors.
Tropical Animal Health and Production | Year: 2013

Seasonal weight loss (SWL), caused by poor quality pastures during the dry season, is the major limitation to animal production in the tropics. One of the ways to counter this problem is to breed animals that show tolerance to SWL. The objective of this study was to understand the effect of feed restriction in milk production and live weight (LW) evolution in two goat breeds, with different levels of adaptation to nutritional stress: the Majorera (considered to be tolerant) and the Palmera (considered to be susceptible). A total of ten animals per breed were used. Animals were divided in four groups (two for each breed): a restricted group (restricted diet) and a control group. LW and milk yield parameters were recorded through a trial that lasted 23 days in total. Overall, there were no significant differences between both restricted groups, regarding neither LW nor milk yield reductions (LW reduction 13 % and milk yield reduction of 87 % for both restricted groups). In what concerns control groups, there were no significant differences between breeds, thought there were different increments at the end of the trial for both breeds regarding LW (6 and 4 %, for Majorera and Palmera, respectively) and milk yield (28 and 8 %, respectively for Majorera and Palmera). The lack of statistically significant differences between Palmera and Majorera LW and milk yields in restricted groups may be due to the fact that the controlled trial does not replicate harsh field conditions, in which Majorera would excel, and the stress induced by those differences. © 2013 Springer Science+Business Media Dordrecht.


Lerias J.R.,University of Lisbon | Lerias J.R.,IBET Institute Biologia Experimental e Tecnologica | Pena R.,Autonomous University of Barcelona | Hernandez-Castellano L.E.,University of Las Palmas de Gran Canaria | And 12 more authors.
Journal of Dairy Research | Year: 2015

Feed restriction, and seasonal weight loss (SWL), are major setbacks for animal production in the tropics and the Mediterranean. They may be solved through the use of autochthonous breeds particularly well adapted to SWL. It is therefore of major importance to determine markers of tolerance to feed restriction of putative use in animal selection. Two indigenous breeds from the Canary Islands, Palmera and Majorera, are commonly used by dairy goat farmers and, interestingly, have different phenotype characteristics albeit with a common ancestry. Indeed, Majorera is well adapted to feed restriction whereas the Palmera is susceptible to feed restriction. In addition, regardless of their importance in dairy production, there are only a limited number of reports relating to these breeds and, to the best of our knowledge, there is no description of their blood metabolite standard values under control conditions or as affected by feed restriction. In this study we analysed the blood metabolite profiles in Majorera and Palmera goats aiming to establish the differential responses to feed restriction between the two breeds and to characterise their metabolite standard values under control conditions. We observed significant differences in creatinine, urea, non-esterified fatty acids (NEFAs), cholesterol, IGF-1 and T3 due to underfeeding. Furthermore, a PCA analysis, revealed that animals submitted to undernutrition could be distinguished from the control groups, with the formation of three separate clusters (Palmera individuals after 22 d of subnutrition (PE22); Majorera individuals after 22 d of subnutrition (ME22) and animals assigned to control conditions (MC0, MC22, PC0 and PC22)), highlighting different responses of the two breeds to undernutrition. Copyright © Proprietors of Journal of Dairy Research 2015


Hernandez-Castellano L.E.,University of Bern | Ferreira A.M.,ITQB UNL Institute Tecnologia Quimica e Biologica | Ferreira A.M.,ICAAM Institute Ciencias Agrarias e Ambientais Mediterranicas | Nanni P.,ETH Zurich | And 9 more authors.
Journal of Proteomics | Year: 2016

Seasonal weight loss (SWL) is a significant limitation to animal production. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. Herein, label free proteomics was used to characterize the effects of SWL in two goat breeds with different levels of adaptation to nutritional stress: Majorera (tolerant) and Palmera (susceptible). Nine Majorera and 10 Palmera dairy goats were used, divided in 4 sets, 2 for each breed: underfed and a control group: Majorera Control (MC), Palmera Control (PC), Majorera Restricted (ME) and Palmera Restricted (PE). At day 22, mammary gland biopsy samples were obtained. Label free proteomic analysis enabled the identification of over 1000 proteins, of which 96 showed differential expression between two of the groups within studied comparisons. Majorera breed showed higher expression of immune system related proteins. In contrast, Palmera breed showed higher expression of proteins related to apoptosis. Results indicate that the two goat breeds have a distinct metabolism reaction to SWL, and that proteins related to the immune system and apoptosis such as cadherin-13, collagen alpha-1, nidogen-2, clusterin and protein s100-A8 could be considered putative candidates as markers of tolerance to SWL. Biological significance: Seasonal weight loss (SWL) is one of the major constraints to animal production in the tropics. We compared the proteomics profiles of two dairy goat breeds with different levels of tolerance to SWL under control and feed restriction conditions using label free proteomics. We have identified over 1000 proteins in the goat mammary gland, of which 96 showed differential expression. Despite the fact that both breeds showed a decrease in the number of proteins related to protein, carbohydrates and fat biosynthesis, the tolerant breed showed higher expression of immune system related proteins compared to the susceptible breed. On the contrary, the susceptible breed had higher expression of apoptosis related proteins indicating that both breeds reacted differently to weight loss and that proteins related to the immune system and apoptosis such as cadherin-13, collagen alpha 1, nidogen-2 or clusterin may be suggested as markers of tolerance to SWL. © 2016 Elsevier B.V.


Lerias J.R.,University of Lisbon | Lerias J.R.,IBET Institute Biologia Experimental e Tecnologica | Pena R.,Autonomous University of Barcelona | Hernandez-Castellano L.E.,University of Las Palmas de Gran Canaria | And 13 more authors.
Journal of Dairy Research | Year: 2015

Feed restriction, and seasonal weight loss (SWL), are major setbacks for animal production in the tropics and the Mediterranean. They may be solved through the use of autochthonous breeds particularly well adapted to SWL. It is therefore of major importance to determine markers of tolerance to feed restriction of putative use in animal selection. Two indigenous breeds from the Canary Islands, Palmera and Majorera, are commonly used by dairy goat farmers and, interestingly, have different phenotype characteristics albeit with a common ancestry. Indeed, Majorera is well adapted to feed restriction whereas the Palmera is susceptible to feed restriction. In addition, regardless of their importance in dairy production, there are only a limited number of reports relating to these breeds and, to the best of our knowledge, there is no description of their blood metabolite standard values under control conditions or as affected by feed restriction. In this study we analysed the blood metabolite profiles in Majorera and Palmera goats aiming to establish the differential responses to feed restriction between the two breeds and to characterise their metabolite standard values under control conditions. We observed significant differences in creatinine, urea, non-esterified fatty acids (NEFAs), cholesterol, IGF-1 and T3 due to underfeeding. Furthermore, a PCA analysis, revealed that animals submitted to undernutrition could be distinguished from the control groups, with the formation of three separate clusters (Palmera individuals after 22 d of subnutrition (PE22); Majorera individuals after 22 d of subnutrition (ME22) and animals assigned to control conditions (MC0, MC22, PC0 and PC22)), highlighting different responses of the two breeds to undernutrition. © Proprietors of Journal of Dairy Research 2015.


PubMed | Icia Instituto Canario Of Investigaciones Agrarias, University of Lisbon, University of Las Palmas de Gran Canaria and Autonomous University of Barcelona
Type: Journal Article | Journal: The Journal of dairy research | Year: 2015

Feed restriction, and seasonal weight loss (SWL), are major setbacks for animal production in the tropics and the Mediterranean. They may be solved through the use of autochthonous breeds particularly well adapted to SWL. It is therefore of major importance to determine markers of tolerance to feed restriction of putative use in animal selection. Two indigenous breeds from the Canary Islands, Palmera and Majorera, are commonly used by dairy goat farmers and, interestingly, have different phenotype characteristics albeit with a common ancestry. Indeed, Majorera is well adapted to feed restriction whereas the Palmera is susceptible to feed restriction. In addition, regardless of their importance in dairy production, there are only a limited number of reports relating to these breeds and, to the best of our knowledge, there is no description of their blood metabolite standard values under control conditions or as affected by feed restriction. In this study we analysed the blood metabolite profiles in Majorera and Palmera goats aiming to establish the differential responses to feed restriction between the two breeds and to characterise their metabolite standard values under control conditions. We observed significant differences in creatinine, urea, non-esterified fatty acids (NEFAs), cholesterol, IGF-1 and T3 due to underfeeding. Furthermore, a PCA analysis, revealed that animals submitted to undernutrition could be distinguished from the control groups, with the formation of three separate clusters (Palmera individuals after 22 d of subnutrition (PE22); Majorera individuals after 22 d of subnutrition (ME22) and animals assigned to control conditions (MC0, MC22, PC0 and PC22)), highlighting different responses of the two breeds to undernutrition.


PubMed | Icia Instituto Canario Of Investigaciones Agrarias, University of Bern, University of Las Palmas de Gran Canaria and New University of Lisbon
Type: Journal Article | Journal: Molecular bioSystems | Year: 2016

Goats are of special importance in the Mediterranean and tropical regions for producing a variety of dairy products. The scarcity of pastures during the dry season leads to seasonal weight loss (SWL), which affects milk production. In this work, we studied the effect of feed-restriction on two dairy goat breeds, with different tolerance levels to SWL: the Majorera breed (tolerant) and the Palmera breed (susceptible). Nuclear magnetic resonance (NMR) was used to compare the metabolome of an aqueous fraction of the mammary gland and milk serum from both breeds. Goats in mid-lactation were divided by breed, and each in two feed-regime groups: the control group and the restricted-fed group (to achieve 15-20% reduction of body weight at the end of the experiment). Milk and mammary gland samples were collected at the end of the experimental period (23rd day). (1)H NMR spectra were collected from the aqueous extract of the mammary gland biopsies and the milk serum. Profiling analysis has led to the identification of 46 metabolites in the aqueous extract of the mammary gland. Lactose, glutamate, glycine and lactate were found to be the most abundant. Analysis of milk serum allowed the identification of 50 metabolites, the most abundant being lactose, citrate and creatine. Significant differences were observed, in mammary gland biopsies and milk serum, between control and restricted-fed groups in both breeds, albeit with no differences between the breeds. Variations seem to be related to metabolism adaptation to the low-energy diet and are indicative of breed-specific microflora. Milk serum showed more metabolites varying between control and restricted groups, than the mammary gland. The Majorera breed also showed more variations than the Palmera breed in milk samples, which could be an indication of a prompt adaptation to SWL by the Majorera breed.


PubMed | Icia Instituto Canario Of Investigaciones Agrarias, Polytechnic University of Valencia, Ecologia y Proteccion Agricola SL and Direccion General de Agricultura Gobierno de Canarias
Type: Journal Article | Journal: Journal of agricultural and food chemistry | Year: 2016

The four-spotted coconut weevil, Diocalandra frumenti Fabricius (Coleoptera: Dryophthoridae), is a small weevil found attacking economically important palm species, such as coconut, date, oil, and Canary palms. Given the scarcity of detection and management tools for this pest, the availability of a pheromone to be included in trapping protocols would be a crucial advantage. Previous laboratory experiments showed evidence for aggregation behavior; thus, our main goal was to identify the aggregation pheromone in this species. The volatile profile of D. frumenti individuals was studied by aeration and collection of effluvia in Porapak-Q and also by solid phase microextraction (SPME) techniques. Moreover, solvent extraction of previously frozen crushed individuals was also performed. All resulting extracts and SPME fibers were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The comparison of male and female samples provided the candidate compound, 5-ethyl-2,4-dimethyl-6,8-dioxabicyclo[3.2.1]octane (multistriatin), whose biological activity was evaluated in olfactometer and field assays.

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