Muller A.P.,ICBS |
Haas C.B.,ICBS |
Camacho-Pereira J.,Federal University of Rio de Janeiro |
Brochier A.W.,ICBS |
And 5 more authors.
Experimental Neurology | Year: 2013
The mitochondrial electron transport system (ETS) is a main source of cellular ROS, including hydrogen peroxide (H2O2). The production of H2O2 also involves the mitochondrial membrane potential (δΨm) and oxygen consumption. Impaired insulin signaling causes oxidative neuronal damage and places the brain at risk of neurodegeneration. We evaluated whether insulin signaling cross-talks with ETS components (complexes I and FoF1ATP synthase) and δΨm to regulate mitochondrial H2O2 production, in tissue preparations from rat brain. Insulin (50 to 100ng/mL) decreased H2O2 production in synaptosomal preparations in high Na+ buffer (polarized state), stimulated by glucose and pyruvate, without affecting the oxygen consumption. In addition, insulin (10 to 100ng/mL) decreased H2O2 production induced by succinate in synaptosomes in high K+ (depolarized state), whereas wortmannin and LY290042, inhibitors of the PI3K pathway, reversed this effect; heated insulin had no effect. Insulin decreased H2O2 production when complexes I and FoF1ATP synthase were inhibited by rotenone and oligomycin respectively suggesting a target effect on complex III. Also, insulin prevented the generation of maximum level of incrementΨm induced by succinate. The PI3K inhibitors and heated insulin maintained the maximum level of incrementΨm induced by succinate in synaptosomes in a depolarized state. Similarly, insulin decreased ROS production in neuronal cultures. In mitochondrial preparations, insulin neither modulated H2O2 production or oxygen consumption. In conclusion, the normal downstream insulin receptor signaling is necessary to regulate complex III of ETS avoiding the generation of maximal incrementΨm and increased mitochondrial H2O2 production. © 2013 Elsevier Inc.
Muller A.P.,ICBS |
Tort A.H.,ICBS |
Gnoatto J.,ICBS |
Moreira J.D.,ICBS |
And 5 more authors.
Behavioural Pharmacology | Year: 2010
Olanzapine and highly palatable diets can alter metabolism and brain function. We investigated the interaction of chronic treatment (4 months) with olanzapine and a cafeteria diet on metabolic parameters, memory tasks (spatial and aversive), the elevated plus maze and locomotor activity induced by d-amphetamine. Male Wistar rats were separated into the following groups: standard diet vehicle, standard diet and olanzapine, cafeteria diet vehicle and cafeteria diet and olanzapine. Olanzapine was administered in the drinking water (approximately 1.5 mg/kg/day), and after 3 days of treatment, the rats exhibited an expected anxiolytic effect and reduced amphetamine-induced hyperlocomotion. After 4 months of treatment, cafeteria diet vehicle and cafeteria diet olanzapine rats exhibited an increased body weight and heavier fat pads compared with the standard diet groups. Olanzapine increased only the epididymal and mesenteric fat pads. The cafeteria diet and olanzapine group showed greater glucose intolerance compared with all other groups. The cafeteria diet altered the effects of chronic olanzapine on the performance in the water maze and inhibitory avoidance tasks. Chronic olanzapine treatment failed to affect amphetamine-induced locomotion and to produce anxiolytic effects in the elevated plus maze task, regardless of the diet. Our results suggest that chronic olanzapine caused an increase in fat pads, which is putatively involved in the etiology of many metabolic diseases. Rats on the cafeteria diet were overweight and exhibited glucose intolerance. We did not observe these effects with olanzapine treatment with the standard diet. Moreover, the chronic treatment regimen caused tolerance to the antipsychotic and anxiolytic effects of olanzapine and seemed to potentiate some of the metabolic effects of the cafeteria diet. The cafeteria diet also modified the effects of chronic treatment with olanzapine on cognitive tasks, which may represent an undesirable effect of poor diets in psychiatric patients. © 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Fuchs S.C.,Federal University of Rio Grande do Sul |
Moreira L.B.,ICBS |
Moreira L.B.,Federal University of Rio Grande do Sul |
Picon R.V.,Federal University of Rio Grande do Sul |
And 2 more authors.
Journal of Asthma | Year: 2010
Background. The epidemiology of asthma has been investigated with questionnaires, such as the International Study of Asthma and Allergies in Childhood protocol. Aim. To investigate the performance of the questions of the International Study of Asthma and Allergies in Childhood questionnaire to diagnose asthma in adolescents. Methods. This is a population-based cross-sectional study of adolescents in the Syndrome of Obesity and Risk Factors for Cardiovascular Disease study. The validity of the asthma symptoms of the International Study of Asthma and Allergies in Childhood protocol was assessed by calculating sensitivity, specificity, positive and negative posttest probabilities, and Youden's Index, taking as a gold standard the history of a medical diagnosis of asthma. Risk ratios (RRs) and 95% confidence intervals (CIs), adjusting for sex and age, were calculated using Cox regression model. Results. In total, 575 adolescents were investigated. Overall, 28.7% reported a lifetime medical diagnosis of asthma, and 40.0% reported at least one episode of wheezing. Ever wheezing had the highest sensitivity (80.6%) for the diagnosis of asthma, compared with the other ISAAC questions. Adolescents who reported ever wheezing were about 8 times more likely (adjusted RR: 8.3; 95% CI: 4.9-14.2) to have ever had asthma, independent of age and sex. Symptoms within the last 12 months (wheezing, cough without cold or respiratory infection, sleep disturbed due to wheezing, wheezing due to exercise, speech limited due to wheezing) had specificity of 92.0% or higher. Dry cough at night without cold or respiratory infection was the strongest independent predictor of asthma (adjusted RR: 8.8; 95% CI: 6.1-12.7). Conclusions. Ever wheezing is the most sensitive indicator of the diagnosis of asthma but falsely identifies a portion of adolescents as asthmatic. Symptoms of asthma in the last 12 months, such as cough without cold or respiratory infection, are rarely positive in the absence of a lifetime asthma diagnosis. The combination of ever wheezing for screening and the presence of other symptoms within the past 12 months to confirm the diagnosis could be an effective strategy to identify the prevalence of asthma in communities. © 2010 Informa ealthcare USA, Inc.
Araujo A.R.,ICBS |
Das Chagas Vallone M.L.D.,PUC Minas |
Dante Perdigao I.C.,PUC Minas |
Amaral S.G.F.,PUC Minas |
And 3 more authors.
Proceedings of the 4th IEEE Global Humanitarian Technology Conference, GHTC 2014 | Year: 2014
The areas of expertise in physical therapy are growing and expanding and the work with human functionality and disability demands the interaction with various areas of knowledge, in addition to innovation and promotion of social capital. In recent years, the technological advances at various areas of the health care system have brought important improvement in the quality of life and in the early detection of changes in health conditions. It was the combined efforts of professionals from different fields, such as the health and exact science, by sharing their experiences and skills and creating conditions for the development of new strategies for solving problems in medicine and biology. This paper highlights the importance of the coalition between physical therapy and engineering, thus, the relationship can grow and fortify, allowing the technological developments to overcome the social problems. The importance of the joint work between physiotherapists and engineers will be demonstrated by the demands identified at the physiotherapy clinic at PUC Minas - Brazil. The combination of the expertise in the area of training of these professionals, to the social issues of inclusion and participation, it is certainly a way to develop concrete actions for the benefit of the society. © 2014 IEEE.
Teixeira A.S.,ICBS |
Ferreira A.S.,ICBS |
Jornal Brasileiro de Reproducao Assistida | Year: 2012
Objective: To describe the different types of studies and different types of issues that are published about Reproduction in JBRA Assisted Reproduction. Method: We conducted a bibliometric study to search for articles published in the journal during the years 2004 to 2011. The categories searched were the type of publication: original article, opinion article, review, summary of thesis statement, Congress abstracts and case reports. Another research item was raised for inclusion in the thematic section of the articles, ie: gynecology, andrology, embryology, reproduction technique, deployment, and others. Results: We found 232 publications between the years 2004-2011. The theme of gynecology accounted for 33.19%. The technology of reproduction 25.86%. Andrology, 18.10%. Studies related to the embryo 9.05%. Implantation 3.88%. Scientific methodology, 0.43% and others 9.49%. Regarding the type of publication, the original articles amounted to 56.48%. Review articles, 23.71%. Opinion articles, 7.32%. Case report, 5.17%. Summary of thesis, 3.01%. Communications, 0.43%. Congress abstracts, 3.88%. Conclusions: The analysis so far, the understanding of the presented research, evaluation methodologies and the presence of various researchers in the journal already show a wider range of Human Reproduction as a specialty and academic discipline. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.
PubMed | ICBS
Type: | Journal: Experimental neurology | Year: 2013
The mitochondrial electron transport system (ETS) is a main source of cellular ROS, including hydrogen peroxide (HO). The production of HO also involves the mitochondrial membrane potential (m) and oxygen consumption. Impaired insulin signaling causes oxidative neuronal damage and places the brain at risk of neurodegeneration. We evaluated whether insulin signaling cross-talks with ETS components (complexes I and FFATP synthase) and m to regulate mitochondrial HO production, in tissue preparations from rat brain. Insulin (50 to 100 ng/mL) decreased HO production in synaptosomal preparations in high Na(+) buffer (polarized state), stimulated by glucose and pyruvate, without affecting the oxygen consumption. In addition, insulin (10 to 100 ng/mL) decreased HO production induced by succinate in synaptosomes in high K(+) (depolarized state), whereas wortmannin and LY290042, inhibitors of the PI3K pathway, reversed this effect; heated insulin had no effect. Insulin decreased HO production when complexes I and FFATP synthase were inhibited by rotenone and oligomycin respectively suggesting a target effect on complex III. Also, insulin prevented the generation of maximum level of m induced by succinate. The PI3K inhibitors and heated insulin maintained the maximum level of m induced by succinate in synaptosomes in a depolarized state. Similarly, insulin decreased ROS production in neuronal cultures. In mitochondrial preparations, insulin neither modulated H2O2 production or oxygen consumption. In conclusion, the normal downstream insulin receptor signaling is necessary to regulate complex III of ETS avoiding the generation of maximal m and increased mitochondrial H2O2 production.