ICAR National Bureau of Plant Genetic Resources

Delhi, India

ICAR National Bureau of Plant Genetic Resources

Delhi, India
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Saha D.,ICAR National Bureau of Plant Genetic Resources | Rana R.S.,ICAR National Bureau of Plant Genetic Resources | Arya L.,ICAR National Bureau of Plant Genetic Resources | Mondal T.K.,ICAR National Bureau of Plant Genetic Resources
Agri Gene | Year: 2017

Germin-like protein (GLP) genes in plants constitute a multigene family that functions in a variety of biological processes, such as plant growth and development and respond to abiotic and biotic stresses. Available genome sequences of foxtail millet (Setaria italica) have facilitated the identification and delineation of the GLP gene family, which is perhaps the first report in any millet crop. A total of 20 SiGLP genes were mapped in six of the nine foxtail millet chromosomes. The majority of these SiGLP genes, except six, clustered into five known germin (GER) groups and revealed group-specific variations in their gene structure and conserved protein motifs. The SiGLP genes shared homologous relationship within and across plant genomes as per their syntenic chromosomal regions, indicating gene duplication and expansion in course of plant evolution. Gene expression dynamics revealed four out of the 20 SiGLP genes having a putative functional role in different tissues. The presence of diverse cis-acting regulatory elements in the putative SiGLP promoter sequences and homology-based 3-D protein structure predictions revealed their possible functional diversity. The present work encompasses a molecular insight into the organization and functions of SiGLP gene family members. This may serve as a platform for functional analysis and their efficient utilizations in genetic improvement of foxtail millet and other related crops. © 2016 Elsevier Inc.

Gawade B.H.,ICAR National Bureau of Plant Genetic Resources | Sirohi A.,Indian Agricultural Research Institute
Indian Journal of Nematology | Year: 2016

Foliar spray and root dip treatments with rose bengal (RB) and DL-amino-n-butyric acid (BABA) @ 25, 50, 100 and 200 μg/ml were given to eggplants and the plants were challenged with root-knot nematode (RKN) juveniles. The elicitors reduced the number of root galls, final population of RKN and increased the growth parameters of eggplant. Both the elicitors were most effective at 200 mg/ml followed by 100 mg/ml concentration in foliar spray as well as root dip treatment. The root dip treatment proved more effective than foliar spray at each concentration. The RB proved more effective in reducing nematode infection than BABA in both the methods. © 2017, Nematological Society of India. All rights reserved.

Dubey S.C.,ICAR National Bureau of Plant Genetic Resources | Singh B.,Indian Agricultural Research Institute | Srinivasa N.,Indian Agricultural Research Institute
Indian Phytopathology | Year: 2017

Wilt caused by Fusarium oxysporum f. sp. ciceris is an important yield limiting disease of chickpea. Cultivation of resistant cultivars is an economical management option for the disease. Six hundred sixty eight genotypes of chickpea were screened against F. oxysporum f. sp. Ciceris in sick field for three years from 2013-14 to 2015-16. The genotypes SCGP-WR 28, H 10-05, GL 10023, IPC 2006-77 and CSJK 72 were found to be resistant and the genotypes H 09-70, H 08-25, H 10-16, DCP 92-3, GNG 1936 and Pusa 212 were moderately resistant. The remaining genotypes showed susceptible reaction against the disease. These genotypes could be cultivated as such or used as resistance source in breeding programme. © Indian Phytopathological Society 2017.

Kaur V.,ICAR National Bureau of Plant Genetic Resources | Behl R.K.,CCS Haryana Agricultural University
Cereal Research Communications | Year: 2017

ADP-glucose pyrophosphorylase (AGPase) activity in the developing grains of two contrasting wheat cultivars WH730 (thermo-Tolerant) and UP2565 (thermo-sensitive) was determined in relation to their allosteric effectors and grain growth. The developing grains (35 days after anthesis) were excised from the middle portion of spikes of wheat genotypes subjected to high temperature, drought and their combination at booting, post-Anthesis and booting+post-Anthesis. The impact of stress treatments was studied by measuring starch content and yield attributes in relation to AGPase activity. AGPase, a key enzyme for starch synthesis, is allosterically activated by 3-phosphoglyceric acid (3-PGA) and inhibited by inorganic phosphate (Pi). Sensitivity of AGPase towards individual and combined high temperature and drought has not been adequately investigated, therefore the present study analyzed AGPase activity, its sensitivity to allosteric effectors under influence of high temperature, drought in order to elucidate the relationship of AGPase with starch accumulation and grain growth. Significant difference in behavior of the enzyme and its allosteric effectors were observed between the two cultivars under high temperature and/or drought. AGPase activity was substantially decreased by high temperature, drought and was found to be positively correlated with the 3-PGA, starch accumulation and yield attributes, while negatively correlated with Pi content. The results showed that effects of high temperature and drought were additive and more severe at booting+post-Anthesis stage. Such studies might help in understanding the control mechanisms associated with the pathway of starch biosynthesis and thus provide chemical means to manipulate starch content vis-à-vis grain yield under heat and drought stress. © 2017 Akadémiai Kiadó, Budapest.

Jacob S.R.,ICAR National Bureau of Plant Genetic Resources | Tyagi V.,Germplasm Exchange Unit | Agrawal A.,Tissue Culture and Cryopreservation Unit | Chakrabarty S.K.,ICAR National Bureau of Plant Genetic Resources | Tyagi R.K.,ICAR National Bureau of Plant Genetic Resources
PLoS ONE | Year: 2015

Food security is a global concern amongst scientists, researchers and policy makers. No country is self-sufficient to address food security issues independently as almost all countries are inter-dependent for availability of plant genetic resources (PGR) in their national crop improvement programmes. Consultative Group of International Agricultural Research (CGIAR; in short CG) centres play an important role in conserving and distributing PGR through their genebanks. CG genebanks assembled the germplasm through collecting missions and acquisition the same from national genebanks of other countries. Using the Genesys Global Portal on Plant Genetic Resources, the World Information and Early Warning System (WIEWS) on Plant Genetic Resources for Food and Agriculture and other relevant databases, we analysed the conservation status of Indian-origin PGR accessions (both cultivated and wild forms possessed by India) in CG genebanks and other national genebanks, including the United States Department of Agriculture (USDA) genebanks, which can be considered as an indicator of Indian contribution to the global germplasm collection. A total of 28,027,770 accessions are being conserved world-wide by 446 organizations represented in Genesys; of these, 3.78% (100,607) are Indian-origin accessions. Similarly, 62,920 Indian-origin accessions (8.73%) have been conserved in CG genebanks which are accessible to the global research community for utilization in their respective crop improvement programmes. A total of 60 genebanks including 11 CG genebanks have deposited 824,625 accessions of PGR in the Svalbard Global Seed Vault (SGSV) as safety duplicates; the average number of accessions deposited by each genebank is 13,744, and amongst them there are 66,339 Indian-origin accessions. In principle, India has contributed 4.85 times the number of germplasm accessions to SGSV, in comparison to the mean value (13,744) of any individual genebank including CG genebanks. More importantly, about 50% of the Indian-origin accessions deposited in SGSV are traditional varieties or landraces with defined traits which form the backbone of any crop gene pool. This paper is also attempting to correlate the global data on Indian-origin germplasm with the national germplasm export profile. The analysis from this paper is discussed with the perspective of possible implications in the access and benefit sharing regime of both the International Treaty on Plant Genetic Resources for Food and Agriculture and the newly enforced Nagoya Protocol under the Convention on Biological Diversity. © 2015 Jacob et al.

Henry R.J.,University of Queensland | Rangan P.,ICAR National Bureau of Plant Genetic Resources | Furtado A.,University of Queensland
Current Opinion in Plant Biology | Year: 2016

Adaptation of cereal crops to variable or changing climates requires that essential quality attributes are maintained to deliver food that will be acceptable to human consumers. Advances in cereal genomics are delivering insights into the molecular basis of nutritional and functional quality traits in cereals and defining new genetic resources. Understanding the influence of the environment on expression of these traits will support the retention of these essential functional properties during climate adaptation. New cereals for use as whole grain or ground to flour for other food products may be based upon the traditional species such as rice and wheat currently used in these food applications but may also include new options exploiting genomics tools to allow accelerated domestication of new species. © 2016 Elsevier Ltd.

Pandey C.D.,ICAR National Bureau of Plant Genetic Resources | Pandey S.,ICAR National Bureau of Plant Genetic Resources
Indian Journal of Horticulture | Year: 2015

Melochia corchorifolia seeds collected from Odisha were tested for germination before conserving in genebank, exhibited physical dormancy due to hard seededness. In order to determine the most suitable method to reduce the hard seeds and promote rapid and uniform germination, seeds were subjected to hot water treatment at different temperatures and duration. Soaking seeds in water at 70°C for 10 min. was found most effective in breaking dormancy and on an average, germination increased from 7 to 96% in two genotypes. Besides germination, seedling vigour, vigour index and electrolyte leakage were also noticed significantly higher in the treated seeds than in untreated seeds. © 2015, Horticulture Society of India. All rights reserved.

Rana J.C.,ICAR National Bureau of Plant Genetic Resources | Chaudhary D.P.,ICAR National Bureau of Plant Genetic Resources
Indian Journal of Genetics and Plant Breeding | Year: 2015

Fifty-one accessions collected from remote areas of NWH region (Himachal Pradesh and Jammu and Kashmir) were evaluated for genetic diversity on the basis of phenotypic and grain quality data. Significant differences were found among accessions for agro-morphological as well as quality traits. These accessions had relatively greater variability for yield per plant (g), grain weight (g), plant height (cm), ear height (cm), kernel rows, number of kernels per row, leaf width (cm) and tryptophan content. The cluster analysis based on the phenotyping and biochemical data divided 51 accessions into four clusters; all accessions from Jammu and Kashmir region grouped into cluster 1. Principal component analysis revealed that plant height, ear height, protein, oil, sugar, starch contents and leaf length (cm) were major contributor towards diversity. The grouping pattern obtained in the cluster analysis and PC biplot was congruent with geographical relationship among the accessions. Accessions such as IC556421 with high protein (13.27%) and sugar (4.53%) content, IC568267 with high oil content (4.94%) and IC568265 with high tryptophan content (0.56%) could be utilized in future research programme. © 2015, Indian Society of Genetics and Plant Breeding. All rights reserved.

Dikshit N.,ICAR National Bureau of Plant Genetic Resources | Dikshit S.,University of Auckland
International Journal of Agricultural and Statistical Sciences | Year: 2016

Cucumis melo L. subsp. agrestis (Naudin) Pangalo is a weedy and feral form of cultivated melon (Cucumis melo L.) and has a widespread occurrence in Africa, Asia and Australian continents. In the present paper, an attempt has been made to study the in situ diversity of fruit and seed characteristics in 23 accessions of Cucumis melo L. subsp. agrestis (Naudin) Pangalo germplasm collected from Central Indian region. Significant variability was observed among the reproductive traits. High coefficient of variation was observed for fruit weight (38.51%) followed by seed breadth (19.19%), fruit length (14.94%), and fruit breadth (12.29%). The studies revealed strong correlation between fruit weight with fruit breadth (0.921) followed by fruit length and fruit weight (0.881), fruit length and seed weight (0.683) and seed length and seed weight (0.657). Based on Ward's minimum variance dendrogram, the accessions can be grouped into two main clusters and there is no distinct pattern of geographic relationship among the accessions studied. Principal component analysis explained that the first principal component accounted for the maximum proportion (63.66%) of the total variability followed by 17.55% by the second Principal components, third component explain 11.58% and the fourth only 3.98% of the variances.

Kumar R.,ICAR National Bureau of Plant Genetic Resources | Radhamani J.,ICAR National Bureau of Plant Genetic Resources
Indian Journal of Horticulture | Year: 2016

Genetic diversity in any crop species is critical for sustaining and thus continuing our efforts towards successful development of desirable varieties. Among the several methods available to assess diversity among germplasm, the SSR markers based DNA profiling is one of the most reliable approaches to assess differences across accessions or varieties unambiguously. In the present study we identified genic-SSR markers in the vegetable Brassica through transferability studies. The identified genic-SSR markers were also tested for their reproducibility across a panel of related but different Brassica species. The genic-SSR markers showing polymorphism across different Brassica species were employed in understanding relationship of vegetable Brassicas with that of both related and distant Brassica species. © 2016 Horticulture Society of India. All Rights reserved.

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