Saha D.,Indian Institute of Natural Resins and Gums ICAR |
Kumar R.,Indian Institute of Natural Resins and Gums ICAR |
Ramani R.,Indian Institute of Natural Resins and Gums ICAR
Indian Journal of Biotechnology | Year: 2013
Albizia lucida Benth. is a forest tree known for timber, gum and tannin, and lac production. Micropropagation of A. lucida can potentially address large-scale production of genetically identical superior genotypes. In the present study, a callus-mediated regeneration protocol has been established from nodal explants of A. lucida for micropropagation. Murashige and Skoog's (MS) medium supplemented with plant growth regulators like thidiazuron (TDZ; 0.5 mμM) and 6- benzylaminopurine (BAP; 2.22 mμM) were found efficient in callus induction and multiplication. From the multiplied greenish and compact organogenic calli, shoots were regenerated best on MS media consisting BAP (8.88 mμM), NAA (1-napthaleneacetic acid; 0.54 mμM) and AgNO3 (5.89 mμM). The excised microshoots were rooted efficiently on 1/2 MS medium supplemented with IBA (indole butyric acid; 9.8 mμM) and BAP (0.11 mμM). The rooted shoots were acclimatized in polybags containing garden soil, sand and FYM (1:1:1) and then transferred into the open. About 66.67% of the plants survived in the end. Thegenetic fidelity of the regenerated plantlets was found identical to the mother plant using SRAP primers.