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Suresh V.,Rajiv Gandhi Center for Biotechnology | Suresh V.,Government Victoria College | Sojan J.,Government Victoria College | Krishna Radhika N.,Central Tuber Crops Research Institute ICAR | Asha V.V.,Rajiv Gandhi Center for Biotechnology
Journal of Ethnopharmacology

Ethnopharmacological relevance: Phyllanthus rheedei Wight is a plant used by Muthuvan tribes of Kerala for treating liver related diseases. Materials and methods: The different extracts of Phyllanthus rheedei were analysed on cell lines were viz, PLC/PRF, Hep3B, FLCII10 and HepG2215 for its anti-HBV property. The analysis was done through ELISA, SQRT-PCR and immuno blotting. The most active extract was then divided in to fractions using HPTLC and the most active fraction was further identified. Results: From the screening experiments it was shown that the ethanol extract of this plant has the maximum activity in lowering the viral markers like HBsAg, HBV Core and HBV X protein and whole virions with comparatively lesser cytotoxicity. The dose responses of this particular extract were further established. Conclusions: This study concluded that the ethanol extract of Phyllanthus rheedei is very much effective in preventing the multiplication of HBV at the cellular level. This study scientifically validated the tribal claim of the use of this plant for severe liver disorders. © 2014 Elsevier Ireland Ltd. All rights reserved. Source

Rizvi S.,Icarda Afghanistan Program | Sharma R.,ICARDA CAC | Srinivas T.,Icarda Afghanistan Program | Srinivas T.,Central Tuber Crops Research Institute ICAR | And 6 more authors.
Acta Agronomica Hungarica

The introduction of new crop varieties is important to improve farm productivity and increase food security in developing countries. This study was conducted to determine the performance of improved varieties of wheat (Triticum aestivum L.), rice (Oryza sativa L.), mungbean [(Vigna radiata (L.) Wilczek] and potato (Solanum tuberosum L.) in comparison to local varieties through farmers' participatory evaluation. The study was conducted during three years (2006 to 2008) with 948 farmers' participatory field trials across 18 districts in three Eastern provinces (Nangarhar, Laghman and Kunar) of Afghanistan. One or more improved varieties of wheat, rice, mungbean and potato were compared to the most popular local variety. Data were recorded on the grain yield of wheat, rice and mungbean, and the tuber yield of potato. On average, the improved varieties outyielded local varieties by 69, 57, 70 and 65% for wheat, rice, mungbean, and potato, respectively. Economic analysis in terms of net benefit demonstrated that the adoption of improved varieties resulted in additional incomes of US$ 1840, 1299, 574 and 790 ha-1 for wheat, rice, mungbean and potato, respectively. These findings underline the importance of on-farm farmers' participatory technology evaluation in developing countries to disseminate new crop varieties to improve farm productivity. Source

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