Carlsbad, CA, United States
Carlsbad, CA, United States
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Patent
Ibis Biosciences | Date: 2017-08-30

The present invention provides methods, compositions, and kits for performing amplification (e.g., whole genome amplification) employing primers that have a 5 restriction site, a 3 random sequence (e.g., a random hexamer), and an identifiable barcode sequence. In certain embodiments, the amplification generates individual amplified sequences that are ligated together to form concatamers containing at least two amplified sequences (e.g., not contiguous on the original target sequence) that are separated by the barcode sequences. In particular embodiments, a plurality of the concatamers are sequenced and aligned with an alignment algorithm that uses the barcode sequences to identify artificial junctions between amplified sequences.


Patent
Ibis Biosciences | Date: 2017-08-30

Provided herein are systems and methods for determining the epigenetic sequences and signatures of bacteria, methods of characterizing bacteria based thereon, and methods of use thereof.


Patent
Ibis Biosciences | Date: 2017-08-30

Apparatuses introduce a liquid over a sample of interest, and related systems and methods utilizing such apparatuses. Apparatuses combine the primary function of a reagent pack with a sample I swab port. The reagent pack portion of the apparatus (e.g., rupturable packs positioned within a main body) holds /stores wet reagents in rupturable packs or reservoirs and also contains dried and or lyophilized reagents and all ancillary items to perform a chemical I biochemical analysis of a sample. The reagent pack is designed in such a fashion such that when activated, rupturable packs (e.g., Blister packs) burst and flow into dried reagent reservoirs and the sample I swab port which contains the sample of interest. The reagent pack is a separate modular piece from any of the microfluidic components but is easily integrated with a microfluidic card (e.g., via a peel and place adhesive I alignment strategy).


Patent
Ibis Biosciences | Date: 2017-07-26

Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).


Patent
Ibis Biosciences | Date: 2017-03-08

The invention relates generally to compositions and methods for disrupting cells (e.g., disrupting cellular and nuclear membranes). In particular, the invention provides modified solid surfaces (e.g., bead surfaces) and their use in disruption of cellular membranes (e.g., during cellular lysis procedures (e.g., for recovery of nucleic acid (e.g., DNA or RNA) from mechanical cell lysis)). Compositions and methods of the invention find use in a wide range of applications including molecular biology and medical science.


Patent
Ibis Biosciences | Date: 2017-08-30

Provided herein are apparatuses, systems, kits and methods for sterilely delivering fluid to an elongated swab. In particular, provided herein are apparatuses configured to sterilely deliver a desired fluid to a swab contained within a swab housing. Apparatuses for delivering fluid to an elongated swab contained within an elongated swab housing are provided. The apparatus comprises a hollow main body having therein a chamber containing a fluid, a hollow applicator body positioned beneath the hollow main body, the hollow applicator body having therein a puncturing element, and an open channel positioned beneath the hollow applicator body, wherein the open channel is configured to connect with an elongated swab housing containing an elongated swab. Puncturing of the chamber results in flow of the fluid through the interior of the hollow main body, through the hollow applicator body, and through the open channel and out the open channel.


Patent
Ibis Biosciences | Date: 2017-05-24

The present invention provides whole blood nucleic acid extraction methods, compositions, and kits, as well as nested isothermal amplification methods, compositions, and kits. In certain embodiments, these methods are applied to detecting Lyme disease, including in patients without classic erythema migrans skin lesions.


The present invention provides systems, methods, and compositions for performing molecular tests. In particular, the present invention provides methods, compositions and systems for generating target sequence-linked solid supports (e.g., beads) using a solid support linked to a plurality of capture sequences and capture primers composed of a 3 target-specific portion and a 5 capture sequence portion. In certain embodiments, the target sequence linked solid support is used in sequencing methods (e.g., pyrosequencing, zero-mode waveguide type sequencing, nanopore sequencing, etc.) to determine the sequence of the target sequence (e.g., in order to detect the identity of a target nucleic acid in sample).


Patent
Ibis Biosciences | Date: 2016-08-29

The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo-Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.


Patent
Ibis Biosciences | Date: 2016-11-07

The present invention provides methods, kits, and compositions for producing single-stranded circular DNA by PCR. In particular, hairpin primers are provided, and methods of use thereof to produce single-stranded circular DNA molecules.

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