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Oeiras, Portugal

Electrophoresis | Year: 2010

This study describes an electrophoretically mediated microanalysis method, suitable for the preclinical evaluation of the hydrolysis of ester drugs by the serum of different animals and for further characterization of human-animal correlation. Dog, cat, cow, horse, sheep, rat and human serum were diluted (25%) in the appropriate buffer and replaced the enzyme solution usually used in electrophoretically mediated microanalysis methods for the study of enzyme kinetics. They were then compared in terms of the ability to hydrolyze acetylthiocholine and butyrylthiocholine (0.25 mM) by in-capillary reaction. Human serum afforded the highest conversion rates (52% butyryltiocholine and 34% acetylthiocholine) followed by horse (31 and 35%), dog (26 and 24%), cat (22 and 14%), rat (11 and 15%) and sheep (8 and 8%). Hydrolysis by bovine serum was negligible. The method is fast (under 8min including rinsing steps), sensitive (under 25 mM substrate could be quantified) and repeatable (RSD≈2%), only requiring minute amounts of sample. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Source

Silva A.C.,IBET ITQB UNL | Peixoto C.,IBET ITQB UNL | Lucas T.,University of Ulm | Kuppers C.,University of Ulm | And 3 more authors.
Current Gene Therapy | Year: 2010

Replication deficient adenovirus vectors are frequently used tools for the delivery of transgenes in vitro and in vivo. In addition, several therapeutic products based on adenovirus are under clinical development. This review outlines adenovirus vector production discussing different vector types, available production cell lines and state of the art of production process development and purification. © 2010 Bentham Science Publishers Ltd. Source

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