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Iba Gmbh | Date: 2015-04-30

The invention provides new methods of isolating target cells using a solid phase, the solid phase comprising a ligand L wherein the ligand L is capable of specifically binding a ligand binding partner LB, the ligand binding partner LB being present in a receptor molecule binding reagent or a multimerization reagent used for isolating target cells. The invention also provides corresponding new arrangements and devices for isolating a target cell from a sample.

PubMed | IBA GmbH and Charles University
Type: | Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology | Year: 2016

In mass cytometry, the isolation of pure lymphocytes is very important to obtain reproducible results and to shorten the time spent on data acquisition. To prepare highly purified cell suspensions of peripheral blood lymphocytes for further analysis on mass cytometer, we used the new CD81+ immune affinity chromatography cell isolation approach. Using 21 metal conjugated antibodies in a single tube we were able to identify all basic cell subsets and compare their relative abundance in final products obtained by density gradient (Ficoll-Paque) and immune affinity chromatography (CD81+ T-catch) isolation approach. We show that T-catch isolation approach results in purer final product than Ficoll-Paque (P values 0.0156), with fewer platelets bound to target cells. As a result acquisition time of 10

Lettau U.,Iba AG
MPT Metallurgical Plant and Technology International | Year: 2014

Only with a precise simultaneous analysis of energy consumption and production data can the efficiency of production plants be optimized. At its hot rolling mill, Stahlwerk Thüringen monitors the energy efficiency of the hydraulic unit for the roll stands by means of a measuring system from Iba AG. The Iba system covers various equipment and machines across the plant. As it also captures production data, it is possible to conduct comprehensive source studies.

Lettau U.,Iba AG
MPT Metallurgical Plant and Technology International | Year: 2013

A Russian producer of longitudinally welded large-diameter steel tubes commissioned a new tube plant at its Chelyabinsk works. German plant manufacturer SMS Meer implemented a data acquisition system from Iba AG for smooth process analysis. Before installing the Iba measurement and automation systems in the entire tube plant, a test phase was implemented to provide theoretical models for the machine operations. In the Russian pipe works, Iba data acquired from the Siemens Simatic-S7 automation system and the MAC-8 automation system from Bosch-Rexroth could be read using an ibaBM-DPMS Profibus-Sniffer. ibaBM-DPM-S is an interface device for fast data exchange on the Profibus. The device offers two modes of operation, the Sniffer mode and the Slave mode and can read up to 512 analogue and 512 digital signals per millisecond. After having recorded the process data, the measured values from the machines were stored as DAT-files and analyzed by means of the ibaAnalyzer.

Iba Gmbh | Date: 2012-07-17

The present invention relates to methods of reversibly staining a target cell. The invention also relates to methods of isolating a target cell or a target cell population that is defined by the presence of at least one common specific receptor molecule. The invention also provides kits that can be used to carry out the methods of the invention.

Disclosed is a bifunctional adapter molecule comprising two binding moieties A and B, the adapter molecule being capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag, wherein the binding moiety A comprises at least two chelating groups K, wherein each chelating group is capable of binding to a transition metal ion, thereby rendering moiety A capable of binding to an oligohistidine affinity tag, and the binding moiety B is an affinity tag other than an oligohistidine tag.

Iba Gmbh | Date: 2013-11-18

The invention concerns novel streptavidin muteins. In one embodiment such a mutein (a) contains at least one mutation in the region of the amino acid positions 115 to 121 with reference to the amino acid sequence of wild type streptavidin as set forth at SEQ ID NO: 15 and (b) has a higher binding affinity than each of (i) a streptavidin mutein that comprises the amino acid sequence Val44-Thr45-Ala46-Arg47 (SEQ ID NO: 98), or (ii) a streptavidin mutein that comprises the amino acid sequence He44-Gly45-Ala46-Arg47 (SEQ ID NO: 99) at amino acid positions 44 to 47, or (iii) wild type-streptavidin (SEQ ID NO: 15) for peptide ligands comprising the amino acid sequence Trp-Ser-His-Pro-Gln-Phe-Glu-Lys (SEQ ID NO: 100).

Iba Gmbh | Date: 2015-04-24

The present invention relates to methods of treating a subject with a population of target cells defined by the presence of a specific receptor molecule on the surface; wherein the population of target cells is isolated by a method of reversibly staining the target cells with a detectable label.

Schmidt T.G.M.,IBA GmbH | Batz L.,IBA GmbH | Bonet L.,IBA GmbH | Carl U.,IBA GmbH | And 6 more authors.
Protein Expression and Purification | Year: 2013

Short peptide affinity tags have become indispensable in protein research. They cannot only be used for affinity purification but also downstream for detection and assay of an arbitrary fused recombinant protein without the need for any prior knowledge of its biochemical properties. Strep-tag®II is particularly popular for providing recombinant proteins at high purity and functionality by using physiological conditions within a rapid one-step protocol. The affinity receptor for Strep-tag®II is affinity engineered streptavidin, named Strep-Tactin®. Strep-tag®II binds to the biotin binding pocket enabling mild competitive elution with biotin derivatives, preferably desthiobiotin, for repeated use of the Strep-Tactin® affinity resins. Fast binding and dissociation kinetics allow comparatively high flow rates throughout column chromatography including elution. Fast dissociation kinetics may be, however, limiting for using Strep-tag®II for direct purification of target proteins from large volumes of diluted extracts like mammalian cell culture supernatants or in assay formats requiring extended washing like ELISA. For this reason, binding characteristics were improved by development of the Twin-Strep-tag® consisting of two Strep-tag®II moieties connected by a short linker. The resulting avidity effect, i.e., the combined synergistic binding of two Strep-tag®II moieties to tetrameric Strep-Tactin®, reduces the off-rate for more steady binding under non-competitive conditions. The addition of a competitor, however, reverses the synergistic avidity effect and, hence, efficient elution capability is preserved. In fact, the Twin-Strep-tag® features all beneficial properties of Strep-tag®II, including efficient elution under gentle competitive conditions, but, due to its higher affinity, additionally enables a more universal use in applications requiring stable binding. © 2013 Elsevier Inc. All rights reserved.

PubMed | IBA GmbH and U.S. National Institutes of Health
Type: | Journal: Protein expression and purification | Year: 2016

Human cannabinoid receptor CB

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