HZI

Braunschweig, Germany
Braunschweig, Germany
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Hemmen K.,HZI | Reinl T.,Helmholtz Center for Infection Research | Buttler K.,University of Gottingen | Behler F.,HZI | And 4 more authors.
Angiogenesis | Year: 2011

Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogeneis. © 2010 Springer Science+Business Media B.V.


Hernandez-Gonzalez M.,CINVESTAV | Alanis A.Y.,University of Guadalajara | Hernandez-Vargas E.A.,HZI
Applied Soft Computing Journal | Year: 2012

Control design for helicopters is a complicated and challenging problem due to the strong inter-couplings and nonlinear uncertainties in the system model. This paper deals with the decentralized control problem for the output trajectory tracking in a Quanser 2 degree of freedom (DOF) helicopter. High order neural network (HONN) is an important technique to approximate non-linearities in the model. Two different discrete-time schemes with a decentralized structure are used. Neural backstepping and neural sliding mode block control techniques are considered in order to control pitch and yaw positions. On one hand, backstepping control divides the whole system into two subsystems which are used to track the pitch and yaw references respectively. Real and virtual controls are approximated by HONNs. On the other hand, block control technique is applied to HONNs which can identify the system helicopter model. Each discrete-time high order neural network is trained on-line with an extended Kalman filter based algorithm. Without the previous knowledge of the plant parameters neither its model, we show via simulations the good performance of both strategies. The block control technique presents slightly better results than backstepping algorithm. © 2011 Elsevier B.V.


PubMed | HZI
Type: Journal Article | Journal: Angiogenesis | Year: 2011

Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogenesis.


News Article | February 22, 2017
Site: news.yahoo.com

FILE PHOTO: An undated image taken with electronic microscope shows EHEC bacteria (enterohaemorrhagic Escherichia coli) in Helmholtz Centre for Infection Research in Brunswick, Germany. REUTERS/Manfred Rohde/Helmholtz-Zentrum fŸr Infektionsforschung (HZI)/File Photo LONDON (Reuters) - Superbug bacteria found in people, animals and food across the European Union pose an "alarming" threat to public and animal health having evolved to resist widely used antibiotics, disease and safety experts warned on Wednesday. A report on antimicrobial resistance in bacteria by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) said some 25,000 people die from such superbugs in the European Union every year. "Antimicrobial resistance is an alarming threat putting human and animal health in danger," said Vytenis Andriukaitis, the EU's health and food safety commissioner. "We have put substantial efforts to stop its rise, but this is not enough. We must be quicker, stronger and act on several fronts." Drug resistance is driven by the misuse and overuse of antibiotics, which encourages bacteria to evolve to survive and develop new ways of beating the medicines. Wednesday's report highlighted that in Salmonella bacteria - which can cause the common and serious food-borne infection Salmonellosis - multi-drug resistance is high across the EU. Mike Catchpole, the ECDC's chief scientist, said he was particularly concerned that some common types of Salmonella in humans, such as monophasic Salmonella Typhimurium, are showing extremely high multi-drug resistance. "Prudent use of antibiotics in human and veterinary medicine is extremely important," he said. "We all have a responsibility to ensure that antibiotics keep working." Resistance to carbapenem antibiotics - usually the last remaining treatment option for patients infected with multi-drug resistant superbugs - was detected for the first time in animals and food, albeit at low levels, as part of EU-wide annual monitoring for the report. It said very low levels of resistance were observed in E. coli bacteria found in pigs and in meat from pigs. Resistance to colistin, another last-resort human antibiotic - was also found at very low levels in Salmonella and E. coli in pigs and cattle, the report said. Marta Hugas, head of EFSA's biological hazards and contaminants unit, noted geographic variations across the European Union, with countries in northern and western Europe generally having lower resistance levels than those in southern and eastern Europe and said this was most likely due to differences in the level of use and overuse of the medicines. "In countries where actions have been taken to reduce, replace and re-think the use of antimicrobials in animals show lower levels of antimicrobial resistance and decreasing trends," she said.

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