Hybrid Catfish Company

Inverness, MS, United States

Hybrid Catfish Company

Inverness, MS, United States
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Abdelrahman H.,Auburn University | ElHady M.,Auburn University | Alcivar-Warren A.,Genomics Usa, Inc. | Allen S.,Virginia Institute of Marine Science | And 62 more authors.
BMC Genomics | Year: 2017

Advancing the production efficiency and profitability of aquaculture is dependent upon the ability to utilize a diverse array of genetic resources. The ultimate goals of aquaculture genomics, genetics and breeding research are to enhance aquaculture production efficiency, sustainability, product quality, and profitability in support of the commercial sector and for the benefit of consumers. In order to achieve these goals, it is important to understand the genomic structure and organization of aquaculture species, and their genomic and phenomic variations, as well as the genetic basis of traits and their interrelationships. In addition, it is also important to understand the mechanisms of regulation and evolutionary conservation at the levels of genome, transcriptome, proteome, epigenome, and systems biology. With genomic information and information between the genomes and phenomes, technologies for marker/causal mutation-assisted selection, genome selection, and genome editing can be developed for applications in aquaculture. A set of genomic tools and resources must be made available including reference genome sequences and their annotations (including coding and non-coding regulatory elements), genome-wide polymorphic markers, efficient genotyping platforms, high-density and high-resolution linkage maps, and transcriptome resources including non-coding transcripts. Genomic and genetic control of important performance and production traits, such as disease resistance, feed conversion efficiency, growth rate, processing yield, behaviour, reproductive characteristics, and tolerance to environmental stressors like low dissolved oxygen, high or low water temperature and salinity, must be understood. QTL need to be identified, validated across strains, lines and populations, and their mechanisms of control understood. Causal gene(s) need to be identified. Genetic and epigenetic regulation of important aquaculture traits need to be determined, and technologies for marker-assisted selection, causal gene/mutation-assisted selection, genome selection, and genome editing using CRISPR and other technologies must be developed, demonstrated with applicability, and application to aquaculture industries. Major progress has been made in aquaculture genomics for dozens of fish and shellfish species including the development of genetic linkage maps, physical maps, microarrays, single nucleotide polymorphism (SNP) arrays, transcriptome databases and various stages of genome reference sequences. This paper provides a general review of the current status, challenges and future research needs of aquaculture genomics, genetics, and breeding, with a focus on major aquaculture species in the United States: catfish, rainbow trout, Atlantic salmon, tilapia, striped bass, oysters, and shrimp. While the overall research priorities and the practical goals are similar across various aquaculture species, the current status in each species should dictate the next priority areas within the species. This paper is an output of the USDA Workshop for Aquaculture Genomics, Genetics, and Breeding held in late March 2016 in Auburn, Alabama, with participants from all parts of the United States. © 2017 The Author(s).


Chatakondi N.G.,Harvest Select Farms | Chatakondi N.G.,U.S. Department of Agriculture | Yant D.R.,Hybrid Catfish Company | Kristanto A.,Auburn University | And 3 more authors.
Journal of the World Aquaculture Society | Year: 2011

This study addresses rapid methods to identify mature channel catfish, Ictalurus punctatus, females for induced spawning with luteinizing hormone releasing hormone analog (LHRHa) and common carp pituitary extract (CPE) and the effects of stage of maturity, hormone dose, season, and cannulation before hormone injection. Hormonal intervention is the most effective method for inducing maturation and spawning in channel catfish × blue catfish, Ictalurus furcatus, hybrids. A total of 80 mature channel catfish were staged for maturity based on secondary sexual characteristics and 20-30 oocytes cannulated to ascertain their maturation stage based on the position of gonadal vesicle. Twenty females were randomly assigned to one of the four hormone regimes (priming + resolving dose): CPE 2 + 8 mg/kg (CPEC); LHRHa 20 + 40 μg/kg (LHRHaL); LHRHa 20 + 60 μg/kg (LHRHaM); and LHRHa 20 + 80 μg/kg (LHRHaH) and a fifth treatment consisted of 20 females selected based on apparent maturity as evidenced by external appearance were injected with CPE, 2 + 8 mg/kg without cannulation (CPEO). Two trials were conducted in early part of the spawning season and two trials in peak season. External methods to identify maturity correlated (r = 0.63) with that of "germinal vesicle position" method to identify the stage of maturity in females. Mean percent of females that ovulated, hatched, and fry produced per kg body weight did not differ (P > 0.05) among the five hormone treatments. The mean percent females that ovulated was higher (P < 0.05) for CPE-induced females that were identified as "excellent" females based on external examination. The performance of cannulated females did not differ (P > 0.05) with that of non-cannulated catfish. The mean egg quality of hormone-induced females and percent of females ovulated in response to hormone treatment established a weak but significant linear relationship (Y = 3.85 + 0.008 × ovulation, r 2 = 0.39, P < 0.05). © by the World Aquaculture Society 2011.


Green C.C.,Louisiana State University | Yant D.R.,Hybrid Catfish Company
Journal of the World Aquaculture Society | Year: 2011

Channel catfish, Ictalurus punctatus, pituitary powder (CP) was prepared and administered to determine the effect of pituitary preparation, head selection criteria, and season of collection on the biological potency of this gonadotropic preparation. Pituitaries were harvested from three catfish processing plants throughout the months of December, February, March, and April to investigate the effect of season on CP potency. Gravid female channel catfish were injected intraperitoneally (IP) with priming (2 mg/kg) and resolving doses (8 mg/kg) of CP treatments, luteinizing hormone-releasing hormone analog (LHRHa), or saline. Pharmaceutical grade Carbopol 971P resin (CAR) was tested as a 1% aqueous dispersion in physiological saline and administered with CP (10 mg/kg) as a IP single injection at a final volume of 2 mL per kg as a slow-release vehicle. Individual females were hand-stripped, and eggs were fertilized with freshly harvested blue catfish, Ictalurus furcatus, spermatozoa. Pituitaries processed with either acetone or ethanol/acetone resulted in significantly different ovulation rates of 35 and 73%, respectively (P≤ 0.01). Ovulation success was not significantly different among CP and LHRHa treatment groups. Ovulation rates were not significantly different among treatment groups of CP collected in April (74%) or December (67%), or collected from male (69%), or small individuals (55%). The shortest latency period among CP treatment was from CP collected in December and from males at 39.1 ± 0.7 and 38.5 ± 0.4 h, respectively. The CAR vehicle treatment performed poorly resulting in an average ovulation rate of 19.0%, with 3.3% viable embryos at 36 h post-fertilization. Pituitaries collected from channel catfish potentially represent a viable spawning aid for the production of hybrid channel × blue catfish. © by the World Aquaculture Society 2011.


Broach J.S.,University of Florida | Ohs C.L.,University of Florida | DiMaggio M.A.,University of Florida | Green C.C.,Louisiana State University | Yant D.R.,Hybrid Catfish Company
North American Journal of Aquaculture | Year: 2015

Abstract: Pinfish Lagodon rhomboides and Pigfish Orthopristis chrysoptera are commonly used as marine baitfish in the southeastern United States. Hormone efficacy studies for spawning induction have been conducted with both species, but spawning aids derived from piscine pituitary extracts have yet to be evaluated. Pituitary extract from Channel Catfish Ictalurus punctatus (CCPE) was chosen to determine its efficacy on spawning induction. The CCPE was dissolved in a saline solution and then injected into female broodfish at 5, 10, 20, or 40 mg/kg. The treated females were then paired with conspecific males receiving half the corresponding female dose. Ovaprim served as a positive control at a dose of 0.5 mL/kg for female broodfish and 0.25 mL/kg for males. A single pair of broodfish was randomly stocked according to treatment into separate 1,600-L tanks within recirculating systems. Broodfish were monitored daily over 72 h after injection for spawning occurrences, and qualitative characteristics including egg and larval morphometrics and viability were quantified. Channel Catfish pituitary extract failed to induce spawning in Pinfish among all dosages administered. All CCPE dosages induced at least one spawn in Pigfish. No significant differences were detected in spawning frequency among treatments although the 10- and 20-mg/kg CCPE dosages and the Ovaprim control each induced ≥50% of Pigfish females to spawn compared with only 16.7% of females treated with the 5- and 40-mg/kg dosages. Floating eggs from Pigfish females in the 10-mg/kg CCPE treatment were significantly larger than eggs produced using either the 20-mg/kg CCPE or Ovaprim treatment. Hatching success, larval survival to first feeding, larval length at hatch and first feeding, and oil droplet diameter of recently hatched Pigfish larvae were significantly greater for those larvae from the Ovaprim treatment than for those from the 10- and 20-mg/kg CCPE treatments. Data from these experiments demonstrate the potential use of CCPE to induce spawning in Pigfish, a popular marine baitfish species. Received June 23, 2014; accepted December 26, 2014 © 2015, © American Fisheries Society.


Grant
Agency: Department of Agriculture | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 80.00K | Year: 2009

The channel x blue hybrid catfish has been documented as a superior animal for use by the catfish industry. To produce the hybrid a hormnone is used to help ovulate the eggs so they can be stripped from the female. Once the eggs are stripped from the female they are fertilized with sperm from the blue catfish and hatched in a manner sililar to channel catfish eggs. Carp pituitary has been used successfully for many years as a spawning aid for catfish. It is very expensive and is produced by a company that depends on commercial fishing to provide the carp. Some years there is a good suppy and other years it is in short supply. Under limited testing, catfish pituitary has been proven to be as effective as Carp pituitary. This project will attemp to optimize the use of this product. Utilizing the catfish pituitary will also provide additional income for the processing plant.

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