Kim K.,Hwayang Dong
17th ITS World Congress | Year: 2010
Installation of video collection devices (CCTV) that can be used to determine the road situation in real time has been expanded recently. This trend reflects the fact that image information is preferred by the road operators and users due to characteristics like immediacy, accuracy, conciseness and visibility. Since the CCTV system that is being operated at the present by the Traffic Information Center is run by operators, however, difficulty in operation will increase as the number of CCTV systems increases. When an accident like an incident occurs, rapid response will be difficult because verification will be late. Since there is functional demand for environmental information like direction information, location information and environment information that are difficult to determine with just image information, text information is displayed simultaneously with the image information. The goal of this paper is to improve service by transferring the passing pattern conveniently in video and images by adding functions which allow vehicle flow to be determined. The improvement method that is being proposed has the advantage of making it possible for the real time traffic information to be understood together with image information. This is accomplished by processing the CCTV image data so that it can be displayed together with the traffic data after analyzing and processing the traffic information that is entered so that traffic data that is collected at the corresponding point is displayed through the CCTV monitor when real time situation of the road is observed through the CCTV system. In addition, there is the advantage that an operator can understand the traffic situation quickly since traffic speed information that is difficult to determine from the CCTV monitor screen is converted to numerical values and displayed on the CCTV screen. In this paper, a video information processing technique for realizing the function of the traffic information display system and schemes to correct the problems that were unearthed were proposed.
Tiwari M.K.,Hwayang Dong |
Tiwari M.K.,Technical University of Denmark |
Kalia V.C.,University of Delhi |
Kang Y.C.,Korea University |
Lee J.-K.,Hwayang Dong
Molecular BioSystems | Year: 2014
Studies on the protein-metal binding sites have mainly focused on the residues immediately surrounding the reacting substrate, cofactors, and metal ions. The contribution of residues in remote layers to the highly optimized microenvironments of catalytic active sites is not well understood. To improve our understanding, the present study examined the role of remote residues on the structure and function of zinc-dependent polyol dehydrogenases. We used an integrated computational and biochemical approach to determine the role of L136 in the third shell of the L-arabinitol 4-dehydrogenase (LAD) from Neurospora crassa. Substitution of L136 with charged (Asp, Lys, or His) and bulky (Trp) side chain amino acids abolished enzyme activity. Whereas the L136A mutant exhibited a 95% decrease in catalytic efficiency (kcat/Km), the L136C mutant exhibited a 39% decrease in kcat/Km. Additionally, molecular docking and dynamic simulations on the mutant (L136A, L136C, L136H, and L136P) complexes showed the loss of crucial H-bonds between G77 and the corresponding mutated residue. It is evident from theoretical and biochemical studies that the L136 is part of the extensive hydrogen bonding network coupled to the reaction catalyzed at the active site. We propose that L136, critically positioned behind the active site residues H78 and E79 in the third shell of LAD, plays a crucial role in modulating catalysis or substrate binding by stabilizing the GHE motif in the LAD active site. © the Partner Organisations 2014.
Tiwari M.K.,Hwayang Dong |
Singh R.,Hwayang Dong |
Singh R.K.,Hwayang Dong |
Kim I.-W.,Hwayang Dong |
And 2 more authors.
Computational and Structural Biotechnology Journal | Year: 2012
Proteins are the most multifaceted macromolecules in living systems and have various important functions, including structural, catalytic, sensory, and regulatory functions. Rational design of enzymes is a great challenge to our understanding of protein structure and physical chemistry and has numerous potential applications. Protein design algorithms have been applied to design or engineer proteins that fold, fold faster, catalyze, catalyze faster, signal, and adopt preferred conformational states. The field of de novo protein design, although only a few decades old, is beginning to produce exciting results. Developments in this field are already having a significant impact on biotechnology and chemical biology. The application of powerful computational methods for functional protein designing has recently succeeded at engineering target activities. Here, we review recently reported de novo functional proteins that were developed using various protein design approaches, including rational design, computational optimization, and selection from combinatorial libraries, highlighting recent advances and successes.