Huzhou Normal University

Huzhou, China

Huzhou Normal University

Huzhou, China

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Ding Z.,Huzhou Normal University | Kong Y.,Huzhou Normal University | Zhang Y.,Huzhou Normal University | Du Z.,East China Normal University | And 4 more authors.
Journal of Fisheries of China | Year: 2017

Dietary conjugated linoleic acids (CLA) have been shown to alter growth rates, improve antioxidant capacity and modulate lipid metabolism in higher animals including some species of fish, but have not been evaluated in economic crustaceans. An 8-weeks feeding trial was conducted to evaluate the effects of dietary CLA on growth, antioxidant capacity and lipid metabolism of oriental river prawn, Macrobrachium nipponense. Five semi-purified diets were supplemented with different levels of CLA (CLA1, 0%; CLA2, 1.5%; CLA3, 3%; CLA4, 4.5%; CLA5, 6%). Each diet was fed to juvenile prawns (mean weight, 0.102±0.0024 g) twice daily to apparent satiation in five replicates. The survival rate was influenced by dietary CLA content, and the survival rate of CLA5 significantly decreased compared with that of CLA1 (P<0.05). No significant difference was observed on weight gain among all groups. Activity of superoxide dismutase (SOD), total antioxidant activity competence (T-AOC) and malondialdehyde (MDA) level in hepatopancreas decreased first, and then increased as CLA level was increased. The SOD activity in prawns fed CLA1 was significantly higher than that of other treatments (P<0.05). The hepatopancreas MDA content was significantly increased when the CLA inclusion was above 4.5% (P<0.05). The total cholesterol concentration (TC) and total triacylglycerol concentration (TG) in plasma were also significantly affected by dietary CLA levels. Gradually decreased TG and TC were observed with the increase of CLA level (P<0.05). The mRNA expressions of carnitine palmityltransferase-1 (CPT1), Acetyl CoA carboxylase (ACC) and scavenger receptor class B type (SR-BI) in hepatopancreas were highly sensitive to dietary CLA. The 4.5% CLA level induced the highest CPT1 and SR-BI mRNA expression levels. Similarly, ACC mRNA expression was also the highest in prawns fed the CLA4 diet, which was significantly higher than that of other groups (P<0.05). These findings demonstrated that (1) CLA inclusion could improve the antioxidant capacity and modulate lipid metabolism; (2) Dietary CLA had little potential for improving growth responses of M. nipponense, and a high proportion (6%) of CLA would reduce the survival rate of prawns.


Ye D.,Shanghai JiaoTong University | Wei K.,Huzhou Normal University | Zhang L.,Shanghai JiaoTong University | Jiang Q.,Shanghai JiaoTong University | And 4 more authors.
American Journal of the Medical Sciences | Year: 2013

AIM: To evaluate the inhibitory effect of a recombinant human papillomavirus (HPV) fusion protein vaccine on oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: An animal model of OSCC was established using human peripheral blood lymphocyte reconstituted nonobese diabetic/severe combined immunodeficiency mice. HPV vaccine was subcutaneously injected into mice after tumor establishment. Tumors and spleens were measured, weighed and stained with hematoxylin and eosin. Lymphocyte subpopulations and cytotoxicity were analyzed with flow cytometric and cytotoxic T lymphocyte assay. RESULTS: The average weight and volume of tumors were significantly lower in the vaccine group than in the control group from day 27. Mice in both groups had high percentages of human CD3 and CD3CD8 T lymphocytes. An elevated percentage of human CD3CD1656 natural killer cells was found in the vaccine group. Moreover, vaccine increased the infiltration of human CD3 and UCHL-1 cells in tumor tissues and enhanced cytotoxicity. CONCLUSIONS: The HPV fusion protein vaccine induces tumor cell death, lymphocyte infiltration and therefore suppresses tumor growth and protects against OSCC. © by the Southern Society for Clinical Investigation.


Ming J.,Huzhou Normal University | Ming J.,Chinese Academy of Fishery Sciences | Ming J.,Nanjing Agricultural University | Xie J.,Chinese Academy of Fishery Sciences | And 4 more authors.
Fish and Shellfish Immunology | Year: 2012

In order to study the effects of dietary emodin, high-dose vitamin C (Vc) and their combination on growth of Wuchang bream (Megalobrama amblycephala Y.) and its resistance to high temperature stress, 1200 healthy Wuchang bream with initial body weight of 133.44 ± 2.11 g were randomly divided into four groups: a control group fed with basal diet (containing 50.3 mg/kg Vc) and three treated groups fed with basal diets supplemented with 60 mg/kg emodin, 700 mg/kg Vc, and the combination of 60 mg/kg emodin + 700 mg/kg Vc, respectively. After feeding for 60 days, the growth performance of Wuchang bream was measured. Then 25 fish per tank were exposed to heat stress of 34 °C. The biochemical parameters of blood and liver, and expression levels of liver two HSP70s mRNA before and after heat stress were determined and the cumulative mortality of each group under heat stress was counted. The results showed that before stress, compared with the control, the weight gain (WG) and specific growth rate (SGR), serum total protein (TP), lysozyme (LSZ), and alkaline phosphatase (ALP) levels, liver superoxide dismutase (SOD) activity and expression level of HSP70 mRNA significantly increased in emodin and Vc groups while feed conversion rate (FCR), serum cortisol (COR), triglyceride (TG) and liver malondialdehyde (MDA) contents decreased (P < 0.05); liver catalase (CAT) activity also significantly increased in emodin group (P < 0.05). Although serum TP, LSZ, and liver HSP70 mRNA levels significantly increased and liver MDA level decreased in combination group (P < 0.05), no synergism was observed.After heat stress, compared with the control, the serum TP, LSZ, ALP levels, liver SOD, CAT activities, and expression levels of HSC70 and HSP70 mRNAs increased in emodin and Vc groups in varying degrees and serum COR, glucose, glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), TG and liver MDA levels decreased to some extent. Although these parameters had similar changing trend as above ones in combination group, it did not show any synergism either. Statistics showed that under heat stress, the cumulative mortalities of emodin and Vc groups, except at 6 h in emodin group, were significantly lower than that of the control (P < 0.05) while the difference between the combination and control groups was not significant (P > 0.05). Thus, the basal diet supplemented with 60 mg/kg emodin or 700 mg/kg Vc could promote the growth of Wuchang bream, reduce FCR, increase non-specific immunity of fish, antioxidant capacity, and two HSP70s mRNA expression levels, and enhance resistance to heat stress in fish. However, the combination of emodin and high-dose Vc showed no better effect. © 2012 Elsevier Ltd.


Zhang W.,Ocean University of China | Wu C.,Ocean University of China | Wu C.,Huzhou Normal University | Mai K.,Ocean University of China | And 2 more authors.
Fish and Shellfish Immunology | Year: 2011

In the present study, the cDNA of heat shock protein 90 from Pacific abalone Haliotis discus hannai Ino (HdhHSP90) was cloned by the combination of homology cloning and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of HdhHSP90 was of 2660 bp, including an open reading frame (ORF) of 2187 bp encoding a polypeptide of 728 amino acids with predicted molecular weight of 84.134 kDa and theoretical isoelectric point of 4.619. BLAST analysis revealed that HdhHSP90 shared high similarity with other known HSP90s, and the five conserved amino acid blocks defined as HSP90 protein family signatures were also identified in HdhHSP90, which indicated that HdhHSP90 should be a cytosolic member of the HSP90 family. The expression levels of HdhHSP90 in haemocytes and hepatopancreas were measured by real-time PCR after Pacific abalone were fed with semi-purified diets containing graded levels of selenium (0.15, 1.32 and 48.70 mg Kg-1) for 20 weeks, respectively. The results showed that the expression levels of HdhHSP90 transcript were significantly up-regulated and reached the maximum (0.47-fold) in hepatopancreas of Pacific abalone fed with optimal dietary Se (1.32 mg Kg-1) (p < 0.05). However, these levels significantly decreased in hepatopancreas at the excessive dietary Se (48.70 mg Kg-1) (p < 0.01). In haemocytes, the expression of HdhHSP90 mRNA increased and reached the maximum (0.96-fold) at the excessive dietary Se (48.70 mg Kg-1) (p < 0.01). It is implied that the expression levels of HdhHSP90 could be affected by dietary Se in hepatopancreas and haemocytes, and HdhHSP90 was potentially involved in the anti-oxidation responses in Pacific abalone H. discus hannai. © 2010 Elsevier Ltd.


PubMed | Henan University and Huzhou Normal University
Type: Journal Article | Journal: Nanoscale research letters | Year: 2014

In this work, the solution-processed CH3NH3PbI3 perovskite/copper indium disulfide (CuInS2) planar heterojunction solar cells with Al2O3 as a scaffold were fabricated at a temperature as low as 250C for the first time, in which the indium tin oxide (ITO)-coated glass instead of the fluorine-doped tin oxide (FTO)-coated glass was used as the light-incidence electrode and the solution-processed CuInS2 layer was prepared to replace the commonly used TiO2 layer in previously reported perovskite-based solar cells. The influence of the thickness of the as-prepared CuInS2 film on the performance of the ITO/CuInS2(n)/Al2O3/(CH3NH3)PbI3/Ag cells was investigated. The ITO/CuInS2(2)/Al2O3/(CH3NH3)PbI3/Ag cell showed the best performance and achieved power conversion efficiency up to 5.30%.


Ding Z.L.,East China Normal University | Ding Z.L.,Huzhou Normal University | Chen L.Q.,East China Normal University | Qin J.G.,Flinders University | And 7 more authors.
Aquaculture Nutrition | Year: 2014

A fatty acid-binding protein (FABP) gene designated as MnFABP10 was cloned and characterized from the freshwater prawn Macrobrachium nipponense. The full-length cDNA of MnFABP10 was 646 bp encoding a 130 amino acid. Real-time quantitative RT-PCR showed that the MnFABP10 gene was expressed in various tissues with the highest expression in the hepatopancreas. The MnFABP10 mRNA levels in the hepatopancreas and ovary of M. nipponense were dependent on the stages of ovarian development. Western blot results revealed a single immunoreactive band with an estimated molecular mass of approximate 14 kDa in the developmental ovary. Then, M. nipponense with an initial body weight of 0.090 ± 0.0010 g were fed with four isonitrogenous and isocaloric diets with different oils, that is, beef tallow (BT), soybean oil (SO), pollack fish oil (FO) and a mixture of fish oil and soybean oil (FO/SO 2 : 1 w/w) for 52 days. The mRNA levels of MnFABP10 in the hepatopancreas were influenced by different lipid sources, with a peak expression observed in prawns fed SO. This study suggests that MnFABP10 may have a putative function in ovary maturation, and its mRNA expression in the hepatopancreas can be regulated by the source of dietary lipids in M. nipponense. © 2013 John Wiley & Sons Ltd.


Wei K.,Huzhou Normal University | Zhang L.,Huzhou Normal University | Yang X.,Huzhou Normal University | Han W.,Huzhou Normal University | And 3 more authors.
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology | Year: 2011

OBJECTIVE: To investigate the expression of cytokeratin 17 (CK17) in oral squamous cell carcinoma (OSCC) as well as its clinical significance.METHODS: Detection of the mRNA level and protein expression of CK17 in the in vitro cellular carcinogenesis model of OSCC, some OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction (PCR), Western blot and immunohistochemistry, respectively.RESULTS: Increased CK17 mRNA level was observed in the HB56 and OSC cell lines compared with the HIOEC using real-time PCR technique. Western blot showed increased CK17 protein expression in all the cell lines compared with the HIOEC. Increased CK17 mRNA and immunoreaction levels were also observed in the cancerous tissue specimens from OSCC patients compared with normal adjacent tissues (P<0.01).CONCLUSION: The significantly increased CK17 gene may be associated with the tumorigenesis and development of OSCC.


PubMed | Huzhou Normal University
Type: Journal Article | Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology | Year: 2011

To investigate the expression of cytokeratin 17 (CK17) in oral squamous cell carcinoma (OSCC) as well as its clinical significance.Detection of the mRNA level and protein expression of CK17 in the in vitro cellular carcinogenesis model of OSCC, some OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction (PCR), Western blot and immunohistochemistry, respectively.Increased CK17 mRNA level was observed in the HB56 and OSC cell lines compared with the HIOEC using real-time PCR technique. Western blot showed increased CK17 protein expression in all the cell lines compared with the HIOEC. Increased CK17 mRNA and immunoreaction levels were also observed in the cancerous tissue specimens from OSCC patients compared with normal adjacent tissues (P<0.01).The significantly increased CK17 gene may be associated with the tumorigenesis and development of OSCC.

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