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Chen Y.,Shanghai University of Traditional Chinese Medicine | Chen Y.,Huzhou Key Laboratory of Molecular Medicine | Gao C.,Laboratory of Clinical Pharmacokinetics | Ma Y.,Shanghai University of Traditional Chinese Medicine | Qiu F.,Laboratory of Clinical Pharmacokinetics
European Journal of Drug Metabolism and Pharmacokinetics | Year: 2013

Guizhi decoction (GZD) is a classic traditional Chinese medicine formula, clinically used for the treatment of influenza, common cold, and other pyretic conditions. A sensitive, specific, and validated liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to investigate the pharmacokinetic properties of cinnamic acid, hippuric acid, paeoniflorin, and glycyrrhetic acid in rat. After single dose oral administration of 7.9 g extract/kg body weight GZD in rats, plasma concentrations of cinnamic acid, hippuric acid, paeoniflorin, and glycyrrhetic acid were measured by LC-MS/MS. Pharmacokinetic parameters were calculated from the plasma concentration-time data. The values of AUC0-t, half-life (t 1/2), and C max were 7.2 ± 2.3 μg h/mL, 1.2 ± 0.3 h, and 9.2 ± 5.2 μg/mL for cinnamic acid, 53 ± 31 μg h/mL, 2.8 ± 2.0 h, and 17 ± 3 μg/mL for hippuric acid, 1.1 ± 0.5 μg h/mL, 1.9 ± 1.1 h, and 0.6 ± 0.3 μg/mL for paeoniflorin, and 11 ± 6 μg h/mL, 6.6 ± 2.5 h, and 0.9 ± 0.6 μg/mL for glycyrrhetic acid, respectively. The results would offer useful information for effective components of GZD in vivo. © 2013 Springer-Verlag France. Source

Xie Y.,Zhejiang University of Science and Technology | Wen X.,Zhejiang University of Science and Technology | Jiang Z.,Zhejiang University of Science and Technology | Fu H.Q.,Zhejiang University of Science and Technology | And 2 more authors.
Clinical Laboratory | Year: 2012

Background: An oncogenic capacity of aquaporins (AQPs) has been recently proposed. They are channel-forming membrane proteins that function as osmotically driven transepithelial and transcellular water. Most recently, overexpression of several AQPs has been reported in different types of human cancer, which indicates that AQPs may play an important role in human carcinogenesis. Methods: In this study, we were going to elucidate the involvement of aquaporin 1 and 4 (AQP1,4) in the metastasis of lung cancer. Results: Expression of AQP1,4 was examined by immunohistochemistry on the twenty lung cancer tissues. AQP1,4 were overexpressed in 65% (13 of 20) and 70% (14 of 20) of adenocarcinoma, while the normal lung tissues were negative. We next investigated the roles of AQP1,4 in the invasion of lung cancer cells by transwell migration assays. It is indicated that migration cells of the AQP1-shRNA or AQP4-shRNA were reduced significantly in comparison to the controls (AQP1- shRNA vs. AQP1-CTL, 5.6% vs. 15.9%, p<0.05; AQP4- shRNA vs. AQP4-CTL, 8.9% vs. 14.8%, p<0.05). From this study, we found AQP1 and AQP4 in lung cancer cell extravasation and spread, which may provide a functional explanation for the expression of AQP1 and AQP4 in lung cancer tissues and lung cancer cell lines. Conclusions: Although further details on the molecular function of AQP1 or 4 related to tumorigenesis remain to be elucidated, our results suggest a potential role of AQP1 or 4 as novel therapeutic targets for the management of lung cancer. Source

Li L.,Huzhou Key Laboratory of Molecular Medicine | Li H.,Huzhou Teachers College
Cancer Biology and Therapy | Year: 2013

Matrix metalloproteinases (MMPs) play important roles in tumor cell proliferation and apoptosis and contribute to tumor growth, angiogenesis, migration, and invasion primarily via extracellular matrix (ECM) degradation and/or the activation of pre-pro-growth factors. Recently, there has been considerable interest in the posttranscriptional regulation of MMPs via microRNAs (miRs). In this review, we highlight the complicated interactive network comprised of different MMPs and their regulating microRNAs, as well as the ways in which these interactions influence cancer development, including tumor angiogenesis, growth, invasion, and metastasis. Based on the conclusive roles that microRNAs play in the regulation of MMPs during cancer progression, we discuss the potential use of microRNA-mediated MMP regulation in the diagnosis and treatment of tumors from the clinical perspective. In particular, microRNA-mediated MMP regulation may lead to the development of promising new MMP inhibitors that target MMPs more selectively, and this approach may also target multiple molecules in a network, leading to the efficient regulation of distinct biological processes relevant to malignant tumors. A thorough understanding of the mechanisms underlying microRNA-mediated MMP regulation during tumor progression will help to provide new insights into the diagnosis and treatment of malignant tumors. © 2013 Landes Bioscience. Source

Chen Y.,Huzhou Key Laboratory of Molecular Medicine | Ma Z.,Huzhou Key Laboratory of Molecular Medicine | Min L.,Huzhou Key Laboratory of Molecular Medicine | Li H.,Huzhou Central Hospital | And 4 more authors.
BioMed Research International | Year: 2015

Lung cancer is one of the most common causes of cancer death, for which no validated tumor biomarker is sufficiently accurate to be useful for diagnosis. Additionally, the metabolic alterations associated with the disease are unclear. In this study, we investigated the construction, interaction, and pathways of potential lung cancer biomarkers using metabolomics pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database and the Human Metabolome Database to identify the top altered pathways for analysis and visualization. We constructed a diagnostic model using potential serum biomarkers from patients with lung cancer. We assessed their specificity and sensitivity according to the area under the curve of the receiver operator characteristic (ROC) curves, which could be used to distinguish patients with lung cancer from normal subjects. The pathway analysis indicated that sphingolipid metabolism was the top altered pathway in lung cancer. ROC curve analysis indicated that glycerophospho-N-arachidonoyl ethanolamine (GpAEA) and sphingosine were potential sensitive and specific biomarkers for lung cancer diagnosis and prognosis. Compared with the traditional lung cancer diagnostic biomarkers carcinoembryonic antigen and cytokeratin 19 fragment, GpAEA and sphingosine were as good or more appropriate for detecting lung cancer. We report our identification of potential metabolic diagnostic and prognostic biomarkers of lung cancer and clarify the metabolic alterations in lung cancer. © 2015 Yingrong Chen et al. Source

Qian F.,Huzhou Key Laboratory of Molecular Medicine | Wang W.,Huzhou Central Hospital | Qiu Z.,U.S. Center for Disease Control and Prevention | Shen Y.,Huzhou Central Hospital | And 3 more authors.
Indian Journal of Pathology and Microbiology | Year: 2013

Objective: To compare the performance of a new tuberculosis-related interferon gamma release assay (TB-IGRA) with that of QuantiFERON-TB Gold In-Tube (QFT-GIT) for TB infection diagnosis in China. Materials and Methods: A total of 458 active TB patients and 378 healthy individuals were enrolled. Among the 458 active TB patients, 395 had pulmonary TB and 63 had extra-pulmonary TB. The blood samples were collected from the active TB patients and health controls; then TB-IGRA and QFT-GIT were used to detect interferon gamma (IFN-) levels. Results: The sensitivity, specificity, positive predictive value, and negative predictive value in TB infection diagnosis for active TB by the TB-IGRA were 83.4%, 94.2%, 94.5%, and 82.4%, respectively. For QFT-GIT, the sensitivity, specificity, positive predictive value, and negative predictive value in TB infection diagnosis for active TB were 81.4%, 97.1%, 97.1%, and 81.2%, respectively. Conclusions: TB-IGRA had a high sensitivity and specificity for TB infection; it could be comparable with the QFT-GIT assay. It might be a powerful assisting tool for TB infection diagnosis in the Chinese clinical setting. Source

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