Huzhou Academy of Agricultural science

Pishan, China

Huzhou Academy of Agricultural science

Pishan, China
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Li Z.,Zhejiang University | Jiang Y.,Zhejiang University | Hua S.,Zhejiang Academy of Agricultural Sciences | Ren Y.,Huzhou Academy of Agricultural science | And 4 more authors.
Plant Growth Regulation | Year: 2013

Arabidopsis seeds accumulate considerable fatty acids (FA/s) from embryo until maturity. The environmental stimuli and the regulatory factors that determine FA accumulation, however, remain unclear. In this study, we investigated the role of DELLAs, the negative regulatory factors in the gibberellin transduction pathway, in seed oil formation in Arabidopsis. Seed morphology, weight-per-thousand seeds, oil content, and FA composition were compared among the seeds of the DELLAga1-3 rga-t2 rgl2-1 (triple), ga1-3 rga-t2 rgl1-1rgl2-1 (quadruple), ga1-3 gai-t6 rga-t2 rgl1-1 rgl 2-1 (penta), and wild type (WT), Landsberg erecta (Ler). Significant differences between the WT and the penta mutant for the above traits were observed. Compared with WT, the penta seeds were characterized by a decrease of 22 % seed oil in term of μg/mg, an increment of 10 % seed oil in term of FA/seed, and an alteration of FA composition. In general, the dynamics of FA accumulation in the penta and WT developing seeds (2-16 days after anthesis, DAA) were not obviously different. However, there was higher FA accumulation in penta seeds from 6 DAA compared with WT. RNA gel blotting indicated that the fatty acid desaturase genes were expressed in seeds as well as in leaves. The different transcript levels of the genes between the penta mutant and WT unveiled a part of the reasons accounting for the differences observed. To our knowledge, this is the first report on the effect of DELLAs on the dynamic process of seed oil formation of a flowering plant. © 2012 Springer Science+Business Media Dordrecht.

Yin Y.-Y.,Nanjing Agricultural University | Yin Y.-Y.,Huzhou Academy of Agricultural science | Liu Y.-J.,Nanjing Agricultural University | Zhang R.-Y.,Nanjing Agricultural University | And 2 more authors.
Acta Prataculturae Sinica | Year: 2016

In this study, three cannulated Holstein cows (590±48 kg liveweight) were used to investigate the effect of acarbose (glucosidase inhibitor) addition (0, 0.5 and 1.0 g/d) on ruminal and hindgut fermentation characteristics in a 3×3 Latin square design. The results showed that acarbose addition decreased the propionate concentration in rumen fluid (P<0.05), increased the ratio of acetate to propionate (P<0.05) but did not affect the concentrations of lactate, acetate, isobutyrate, butyrate, isovalerate, valerate, total volatile fatty acid, ammonia-nitrogen, and pH value compared with the control (P>0.05). Acarbose addition decreased the pH value and ammonia-nitrogen levels (P<0.05) and increased the concentrations of lactate, butyrate, isovalerate (P<0.05) in feces, but no differences were observed in the concentration of acetate, propionate, isobutyrate, valerate, total volatile fatty acid and the ratio of acetate to propionate among the three groups. In general, our data revealed that acarbose addition may not be useful for preventing the occurrence of ruminal acidosis in the long term. Additionally, acarbose addition may increase hindgut fermentation and produce detrimental effects on the hindgut health of dairy cattle. Copyright © ACTA PRATACULTURAE SINICA.

Hua S.,Zhejiang Academy of Agricultural Sciences | Yu H.,Zhejiang Academy of Agricultural Sciences | Zhang Y.,Zhejiang Academy of Agricultural Sciences | Lin B.,Zhejiang Academy of Agricultural Sciences | And 4 more authors.
Australian Journal of Crop Science | Year: 2012

Determination ofdynamics of the physiological traits that affect the seed oil content in canola(Brassica napusL.)is essential forhigh seed oil content breeding programs.The main purpose of the present experiments was to explore the relationship between seed oil content and carbohydrates, N and P content of various canola aboveground tissues during flowering stage using two recombinant inbred lines RILS. Two field experiments were performed simultaneously in both 2010 and 2011. In experiment 1, seed yield and quality were compared between two RILs with contrasting seed oil content, that is, high oil content line (HOCL) and low oil content line (LOCL) at 50.4% and 41.4%, respectively. In experiment 2, our resultsshowed theHOCLproduced markedly more biomass in the reproductive organsby 9.9% and 30% at 35 days after anthesis (DAA) in 2010 and 2011, respectively. Furthermore, compared with LOCL, HOCLaccumulated higher fructose,sucrose, and P contentin the reproductive organs atthelate flowering stage, withan averageincrement of 85.0%, 31.1%, and 12.4%at 35 DAA, respectively. However, the mean N content in the reproductive organsof HOCLwas 17.27% lower than that in LOCLat 35DAA. These results reveal the importance of thehigher reproductive organ C/N ratio in the regulation of thehigher seed oil content ofHOCL.Therefore, the C/N ratio of the reproductive organ may be a useful physiological indicator to screen canola lines for high seed oil content in future breeding program.

Ren Y.,Huzhou Academy of Agricultural science | Zhu J.,Huzhou Academy of Agricultural science | Hussain N.,Zhejiang University | Ma S.,Huzhou Academy of Agricultural science | And 3 more authors.
Canadian Journal of Plant Science | Year: 2014

Seedling quality is an essential indicator for seed yield in canola, which is affected by many factors, including seedling age. Two field experiments were conducted to compare canola seedling quality and seed yield on 30-, 35-, 40-, 45-, 50-, 55-, and 60-d-old seedlings in 2011 and 2012. The relationship between seedling quality traits and seed yield of different seedling ages was also analyzed. Results revealed that the highest seed yield obtained from 40-d-old seedlings was attributed to more branches and siliques per plant. The negative effect of young seedlings (30-d-old) on seed yield was greater than that of old seedlings (60-d-old). The reduction rates in seed yield on the 30- and 60-d-old seedlings were 25.7 and 18.2%, respectively, compared with the 40-d-old seedlings. Increased root neck diameter, green leaf number, shoot, and root dry matter was the case on 40-d-old seedling transplanted plants compared with other ages. However, the increase was larger in the old seedlings than in the young seedlings. On average, the shoot and root dry weights of the 30-d-old seedlings were 1.9 and 1.7% of those in the 60-d-old seedlings. However, correlation analysis revealed that the seedlings with the highest shoot and root dry matter did not necessarily obtain the highest seed yield. Factor analysis suggested that the effects of root neck diameter and green leaf number on seed yield were more pronounced than those of shoot and root dry matter. Therefore, high seed yield in canola could be defined in terms of optimum green leaf numbers and branches per plant. © 2014, Agricultural Institute of Canada. All rights reserved.

Hua S.,Zhejiang Academy of Agricultural Sciences | Lin B.,Zhejiang Academy of Agricultural Sciences | Hussain N.,Zhejiang University | Zhang Y.,Zhejiang Academy of Agricultural Sciences | And 4 more authors.
International Journal of Agriculture and Biology | Year: 2014

Delayed planting causes substantial reduction in crop yield; however, information on physiological regulation of yield reduction is scarce. A field experiment was conducted using four planting dates as treatments, namely, early (September 15); optimal (September 25); late (October 25); and very late (November 15) in 2011 and 2012 to unravel the impact of delayed planting date on canola yield and physiological indexes, including tissue dry matter and carbohydrate profile partitioning. Optimal planting date was considered as a control. Results showed that canola seed yields on late and very late planting dates were severely decreased by 30% and 70%, respectively, compared with those on the optimal planting date because of a decrease in the number of branches and siliques per plant and seed weight. Delayed sowing reduced the inflorescence productivity due to less biomass from initial flowering. Inflorescence sugar profile including fructose, glucose, and sucrose showed more content on the late planting than that on the early and optimal planting date at 28 days after anthesis (DAA) (peak point) indicating they were possibly assigned to gain more biomass. However, starch content in the inflorescence on the early and optimal planting date was much higher than that in the late plantation at 28 DAA, indicating that the photoassimilates were mainly used as seed reserves such as lipids. © 2014 Friends Science Publishers.

Hua S.,Zhejiang Academy of Agricultural Sciences | Zhang Y.,Zhejiang Academy of Agricultural Sciences | Yu H.,Zhejiang Academy of Agricultural Sciences | Lin B.,Zhejiang Academy of Agricultural Sciences | And 4 more authors.
International Journal of Agriculture and Biology | Year: 2014

The mechanical harvesting of canola seeds requires a suitable plant height. The appropriate time of paclobutrazol application can reduce canola plant height. To evaluate the best application time of paclobutrazol, a field experiment was conducted with the paclobutrazol applied at stalk heights of 10, 20, 30, 40 and 50 cm. Plants without paclobutrazol application were taken as control. The results indicated that the plant height was reduced by 27% with paclobutrazol applied at 10 cm stalk height as compared with the control. The seed yield was significantly improved by mean increment of 21%. The increased branching ability contributed to yield improvement. The total soluble sugar, sucrose, and starch content in the stem, leaf and bud organs were significantly increased by paclobutrazol application at the initial flowering stage. Paclobutrazol enhanced the sucrose synthesis by sucrose phosphate synthase as well as the catalysis via sucrose synthase, neutral invertase, and in particular acid invertase. The amount of carbohydrates during harvesting remained significantly lower in the root after paclobutrazol treatment, thereby indicating the high-efficiency of carbohydrate utilization by the canola plants in comparison with the control plants. Thus, paclobutrazol applied at 10 cm stalk height takes advantages to control plant height and improve seed yield through enhancing carbohydrate utilization efficiency, which is useful in future for canola mechanical harvesting. © 2014 Friends Science Publishers.

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