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São Paulo, Brazil

Losano J.D.A.,University of Sao Paulo | Angrimani D.S.R.,University of Sao Paulo | Pereira R.J.G.,University of Sao Paulo | Rocha A.M.,University of Michigan | And 6 more authors.
Andrologia | Year: 2015

Due to homologies between the chicken egg perivitelline membrane with mammalian zona pellucida proteins, spermatozoa of several species are able to bind to this membrane. However, adequate standardisation is required to attest possible applications of this technique for semen evaluation of a given species. Therefore, we thawed and divided cryopreserved semen samples into two aliquotes, one kept in water bath at 37 °C (thawed) and the other submitted to snap-freezing to damage sperm cells (dead spermatozoa). Aliquotes were mixed into different ratios of thawed:dead cells and analysed for motility, membrane and acrosomal integrity, and mitochondrial activity. In parallel, chicken egg perivitelline membranes were inseminated with these ratios, and the number of spermatozoa bound per mm2 of membrane was assessed by conventional microscopy (CM) and computer-assisted sperm analysis (CASA). Linear regression showed high correlation between thawed:dead sperm ratio and number of spermatozoa bound to the membrane (CM: r2 = 0.91 and CASA: r2 = 0.92 respectively). Additionally, positive correlations were found between the number of spermatozoa bound to the membrane and acrosomal integrity, membrane integrity, mitochondrial activity and motility. These findings indicate that sperm-egg-binding assay associated with CASA is a reliable, practical and inexpensive method for examining the fertilising capacity of cryopreserved bull semen. © 2014 Blackwell Verlag GmbH. Source


Zanatta A.,Huntington Medicina Reprodutiva | Rocha A.M.,Huntington Medicina Reprodutiva | Pereira R.M.A.,Huntington Medicina Reprodutiva | Taylor H.S.,Yale University | And 4 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2010

Purpose: Endometriosis and its associated infertility have been the object of continuous research for over a century. To understand the molecular mechanisms underlying the disease, it has become necessary to determine the aspects of its etiology that are not explained by the retrograde menstruation theory. This could in turn elucidate how various clinical and surgical treatments might affect the evolution and remission of the disease. Methods: This review is focused on the most recent clinical and laboratory findings regarding the association of HOXA10 with endometriosis and infertility. Result: The homebox (Hox/HOX) proteins are highly conserved transcription factors that determine segmental body identities in multiple species, including humans. Hoxa10/HOXA10 is directly involved in the embryogenesis of the uterus and embryo implantation via regulation of downstream genes. Cyclical endometrial expression of Hoxa10/HOXA10, with a peak of expression occurring during the window of implantation, is observed in the adult in response to estrogen and progesterone. Women with endometriosis do not demonstrate the expected mid-luteal rise of HOXA10 expression, which might partially explain the infertility observed in many of these patients. Recent studies also demonstrated HOXA10 expression in endometriotic foci outside the Müllerian tract. Conclusions: Multiple lines of evidence suggest that the actions of the homeobox A10 (Hoxa10/HOXA10) gene could account for some aspects of endometriosis. © 2010 Springer Science+Business Media, LLC. Source


Alegretti J.R.,Huntington Medicina Reprodutiva | Alegretti J.R.,Federal University of Sao Paulo | Rossi A.L.S.,Huntington Medicina Reprodutiva | Riboldi M.,Huntington Medicina Reprodutiva | And 5 more authors.
Jornal Brasileiro de Reproducao Assistida | Year: 2013

Objetive: To evaluate rate blastocyst aneuploidy after Array-CGH in different age groups with different infertility history in couples submitted to IVF. Methods: 598 cycles were evaluated and 2084 blastocysts between 11/2010-01/2013. Embryo biopsy was performed on blastocyst and sample was performed to Array-CGH analysis. Patients were divided in 4 groups according to maternal age: A)≤34years old(yo); B)35-37yo; C)38-40yo and D)≥41yo. The couples infertility history were classified in 6 groups: 1)+1 IVF failure (IVFF) and +1 abortion (AB); 2)1 IVFF without PM; 3)1AB without IVFF; 4)+2 IVF without PM; 5)+2 AB without IVFF and 6) no failures. The aneuploidies were divided in 4 groups: I) Monosomy; II)Trisomy; III)Multiple and IV)Complex. Were also verified the rate of deletions, duplications and DNA degradation. Results: Analysis of aneuploidy rate by age groups showed statistical differences in group 1 [A×D (p = 0.041) and B×C (p = 0.005)], Group 3 [A×B (p = 0.05), A×C (p=0.05) and A×D (p=0.02)], Group 4 [A×D (p=0.011), B×D (p=0.002) and C×D (p=0.03)], Group 5 [A×C (p=0.013), A×D (p=0.001) and C×D (p=0.018)] and Group 6 [A×C (p=0.001), A×D (p=0.001), B×C (p = 0.013) and B×D (p = 0.001)]. Group 2 did not differ between age groups. However, the comparative analysis between the types of aneuploidy found in all age groups and infertility factors showed no statistically significant differences Conclusions: Our study suggests that the presence of embryonic chromosomal abnormalities is not related with the infertility history. Similar maternal age groups with different backgrounds showed the same incidence of the chromosomal abnormalities. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida. Source


Chamie L.P.,Fleury Medicina e Saude | Pereira R.M.A.,Huntington Medicina Reprodutiva | Zanatta A.,Huntington Medicina Reprodutiva | Serafini P.C.,Huntington Medicina Reprodutiva | Serafini P.C.,University of Sao Paulo
Radiographics | Year: 2010

Deeply infiltrating endometriosis (DIE) is a common gynecologic disease that is characterized by a difficult and delayed diagnosis. Radiologic mapping of the DIE lesion sites is crucial for case management, patient counseling, and surgical planning. Transvaginal ultrasonography (US) is the initial imaging modality for investigating DIE and has been the focus of several recent studies. DIE typically manifests at imaging as hypoechogenic nodules throughout the affected sites and thickening of the intestinal wall, with some lesions showing a mixed pattern due to cystic areas. Transvaginal US performed after bowel preparation improves the ability to diagnose intestinal lesions and provides invaluable details, including which layers of the intestine are affected and the distance between the lesion and the anal border. It is vital that radiologists be familiar with the technical aspects of this modality and with the US manifestations of DIE lesions. Transvaginal US performed after bowel preparation should be the first-line imaging modality for the evaluation of women with suspected endometriosis. © RSNA, 2010. Source


Chamie L.P.,Diagnostic Imaging | Blasbalg R.,Diagnostic Imaging | Blasbalg R.,University of Sao Paulo | Ricar-do Mendes A.P.,Huntington Medicina Reprodutiva | And 4 more authors.
Radiographics | Year: 2011

Endometriosis is a common multifocal gynecologic disease that manifests during the reproductive years, often causing chronic pelvic pain and infer-tility. It may occur as invasive peritoneal fibrotic nodules and adhesions or as ovarian cysts with hemorrhagic content. Although findings at physical examination may be suggestive, imaging is necessary for definitive diagnosis, patient counseling, and treatment planning. The imaging techniques that are most useful for preoperative disease mapping are transvaginal ultrasonography (US) after bowel preparation, and magnetic resonance (MR) imaging. Initial transvaginal US is a reliable technique for detecting rectosigmoid endometriotic lesions. MR imaging is indicated as a comple-mentary examination in complex cases of endometriosis with extensive adhesions and ureteral involvement. Peritoneal endometriotic implants are typically hypoechoic on transvaginal US images and demonstrate low signal intensity on T2-weighted MR images. Endometriotic implants most commonly are found in retrocervical and rectosigmoid sites, followed by the vagina, bladder, and ureters. Cysts with low-level internal echoes and echogenic peripheral foci at transvaginal US are suggestive of endome-triomas. MR imaging has high specificity for identifying endometriomas, which are characterized by high signal intensity on T1-weighted images and low signal intensity on T2-weighted images. Correlation of the radiologic imaging features of endometriotic lesions with their laparoscopic appear-ances may help improve individual proficiency in the radiologic diagnosis of endometriosis. © RSNA, 2011. Source

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