Chrysin, a natural and biologically active flavonoid, influences a murine leukemia model in vivo through enhancing populations of T-and B-cells, and promoting macrophage phagocytosis and NK cell cytotoxicity
Lin C.-C.,Fong Yuan Hospital |
Lin C.-C.,Hunkuang University |
Yu C.-S.,China Medical University at Taichung |
Yang J.-S.,China Medical University at Taichung |
And 6 more authors.
In Vivo | Year: 2012
Chrysin (5,7-dihydroxyflavone), a natural and biologically active flavonoid found in plants, possesses many biological activities and anticancer effects. However, there is no available evidence regarding the antileukemia responses to chrysin in a mouse model. We hypothesized that chrysin affects murine WEHI-3 leukemia cells in vitro and in vivo. The present study showed that chrysin at concentrations of 5-50 μM reduced the cell viability in concentration- and time-dependent manners. In an in vivo study, WEHI-3 leukemic BALB/c mice were established in order to determine antileukemia activity of chrysin. Our results revealed that chrysin increased the percentage of CD3 (T-cell maker), CD19 (B-cell maker) and Mac-3 (macrophages) cell surface markers in treated mice as compared with the untreated leukemia group. However, chrysin did not significantly influence the level of CD11b (a monocyte maker) in treated mice. Moreover, there was a significant increase in phagocytosis by macrophages from peripheral blood mononuclear cells, but no effect in those from the peritoneal cavity in leukemic mice after chrysin treatment. Isolated splenocytes from chrysin-treated leukemic mice demonstrated an increase of natural killer (NK) cell cytotoxicity. Based on these observations, chrysin might exhibit antileukemia effects on a murine WEHI-3 cell line-induced leukemia in vivo.
Wang C.-J.,Cheng Hsin General Hospital |
Chen C.-C.,HunKuang University |
Tsay H.-J.,National Yang Ming University |
Chiang F.-Y.,National Yang Ming University |
And 3 more authors.
Journal of Biomedical Science | Year: 2013
Background: Microglial inflammation may significantly contribute to the pathology of Alzheimer's disease. To examine the potential of Cudrania cochinchinensis to ameliorate amyloid β protein (Aβ)-induced microglia activation, BV-2 microglial cell line, and the ramified microglia in the primary glial mixed cultured were employed. Results: Lipopolysaccharide (LPS), Interferon-γ (IFN-γ), fibrillary Aβ (fAβ), or oligomeric Aβ (oAβ) were used to activate microglia. LPS and IFN-γ, but not Aβs, activated BV-2 cells to produce nitric oxide through an increase in inducible nitric oxide synthase (iNOS) expression without significant effects on cell viability of microglia. fAβ, but not oAβ, enhanced the IFN-γ-stimulated nitric oxide production and iNOS expression.The ethanol/water extracts of Cudrania cochinchinensis (CC-EW) and the purified isolated components (i.e. CCA to CCF) effectively reduced the nitric oxide production and iNOS expression stimulated by IFN-γ combined with fAβ. On the other hand, oAβ effectively activated the ramified microglia in mixed glial culture by observing the morphological alteration of the microglia from ramified to amoeboid. CC-EW and CCB effectively prohibit the Aβ-mediated morphological change of microglia. Furthermore, CC-EW and CCB effectively decreased Aβ deposition and remained Aβ in the conditioned medium suggesting the effect of CC-EW and CCB on promoting Aβ clearance. Results are expressed as mean ± S.D. and were analyzed by ANOVA with post-hoc multiple comparisons with a Bonferroni test. Conclusions: The components of Cudrania cochinchinensis including CC-EW and CCB are potential for novel therapeutic intervention for Alzheimer's disease. © 2013 Wang et al.; licensee BioMed Central Ltd.
Lin C.-C.,Fong Yuan Hospital |
Lin C.-C.,Hunkuang University |
Lin J.-J.,China Medical University at Taichung |
Wu P.-P.,China Medical University at Taichung |
And 8 more authors.
In Vivo | Year: 2013
Wogonin, a natural and biologically-active flavonoid found in plants, has been reported to exhibit anticancer effects on several cancer cell types. However, there is no available information regarding the responses to wogonin in leukemia mouse models. At concentrations of 10- 200 μM, wogonin reduced the percentage of viable WEHI-3 cells in a concentration-dependent manner. In an in vivo study, WEHI-3 cells were intraperitoneally injected into normal BALB/c mice for establishing leukemic BALB/c mice to determine the anti-leukemia activity of wogonin. Wogonin increased the survival rate and the body weight of leukemic mice when compared to vehicle (olive oil)-treated groups. Furthermore, the results also revealed that wogonin increased the percentage of cluster of differentiation-3 CD3 (T-cell marker) and CD19 (B-cell marker) but reduced that of Mac- 3 (macrophages) and CD11b (monocytes) cell surface markers in treated mice as compared with the untreated leukemia group. Based on these observations, wogonin might exhibit anti-leukemia effects on murine WEHI-3 cell lineinduced leukemia in vivo.
Wang M.L.,Hunkuang University |
Liu Y.C.,Institute of Chemical Technology |
Lin S.J.,National Tsing Hua University
Journal of the Chinese Institute of Engineers, Transactions of the Chinese Institute of Engineers,Series A/Chung-kuo Kung Ch'eng Hsuch K'an | Year: 2010
In this work, a novel phase-transfer catalyst (4-methyl)methylbenzoatyl- phosphonium bromide (BrPPh3CH2(C6H 4)COOCH3, MBPB), which can be applied to synthesize specialty chemicals via Wittig reaction, is first prepared from the two-phase solution. The MBPB catalyst is first synthesized from the organic-phase reaction of (4-methyl)methyl benzoate (BME) with triphenyl phosphine (PPh3) to produce the desired catalyst (4-methyl)methylbenzoatyl phosphonium bromide (MBPB), which is then transferred to the aqueous phase. Thus, the reaction of synthesizing MBPB was carried out in an aqueous solution/organic solvent two-phase medium. A kinetic model of the reaction was also developed. The investigation considered the effects on the conversion of reaction conditions, including organic solvents, agitation speed, the concentration of triphenyl phosphine (PPh3), the concentration of (4-methyl)methyl benzoate (BME), and the temperature. It was found that the rate constants for the two-phase solution (kT) is larger than that of the rate constant (ks) for the single-phase solution. In addition, there is discussion of the distribution coefficient of MBPB between aqueous phase and organic phase, in which its value was measured experimentally, as affected by the concentration of MBPB in the aqueous phase and in the organic phase.