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Chen D.-C.,National Central University | Chen D.-C.,Taiwan Landseed Hospital | Chen L.-Y.,National Central University | Ling Q.-D.,National Central University | And 13 more authors.
Biomaterials | Year: 2014

The purification of human adipose-derived stem cells (hADSCs) from human adipose tissue cells (stromal vascular fraction) was investigated using membrane filtration through poly(lactide-co-glycolic acid)/silk screen hybrid membranes. Membrane filtration methods are attractive in regenerative medicine because they reduce the time required to purify hADSCs (i.e., less than 30 min) compared with conventional culture methods, which require 5-12 days. hADSCs expressing the mesenchymal stem cell markers CD44, CD73, and CD90 were concentrated in the permeation solution from the hybrid membranes. Expression of the surface markers CD44, CD73, and CD99 on the cells in the permeation solution from the hybrid membranes, which were obtained using 18 mL of feed solution containing 50 × 104 cells, was statistically significantly higher than that of the primary adipose tissue cells, indicating that the hADSCs can be purified in the permeation solution by the membrane filtration method. Cells expressing the stem cell-associated marker CD34 could be successfully isolated in the permeation solution, whereas CD34+ cells could not be purified by the conventional culture method. The hADSCs in the permeation solution demonstrated a superior capacity for osteogenic differentiation based on their alkali phosphatase activity, their osterix gene expression, and the results of mineralization analysis by Alizarin Red S and von Kossa staining compared with the cells from the suspension of human adipose tissue. These results suggest that the hADSCs capable of osteogenic differentiation preferentially permeate through the hybrid membranes. © 2014 Elsevier Ltd.


Wu C.-H.,National Central University | Lee F.-K.,Cathay General Hospital | Suresh Kumar S.,National Central University | Ling Q.-D.,Cathay Medical Research Institute | And 10 more authors.
Biomaterials | Year: 2012

Human adipose-derived stem cells (hADSCs) were purified from a suspension of human adipose tissue cells (stromal vascular fraction) by the conventional culture method and by membrane filtration through polyurethane (PU) foam membranes. hADSCs can be obtained from a suspension of human adipose tissue cells using the membrane filtration method in less than 30 min, whereas the conventional culture method requires 5-12 days. hADSCs that express the mesenchymal stem cell markers CD44, CD73, and CD90 were concentrated in the recovery solution from the PU membranes; no hADSCs were isolated in the permeate. After filtration, the cells expressing the mesenchymal stem cell markers were 3-4.5 times more concentrated than in the initial suspension of human adipose tissue cells, with the level of concentration depending on the surface modification of the PU membrane. Cells expressing the stem cell-associated marker CD34 could be successfully isolated in the recovery solutions, whereas CD34+ cells could not be purified by the conventional culture method. The hADSCs in the recovery solution demonstrated a superior capacity for osteogenic differentiation than did the cells in the suspension of human adipose tissue cells. These results suggested that the hADSCs with the capability for osteogenic differentiation adhered to the PU membranes. © 2012 Elsevier Ltd.


Hsieh C.-H.,Fu Jen Catholic University | Hsieh C.-H.,Chung Shan Hospital | Hsieh C.-H.,Hungchi Women and Childrens Hospital | Chang W.-C.,China Medical University at Taichung | And 4 more authors.
International Journal of Gynecology and Obstetrics | Year: 2012

Objective: To investigate the association between the prevalence of urinary incontinence and parity or mode of delivery among Taiwanese women aged 60 years or older. Methods: Between July 1999 and December 2000, a nationwide epidemiologic study was conducted in Taiwan among 2410 women selected by a multistage random sampling method. Face-to-face interviews with 1517 women were conducted. The relationship between the prevalence of urinary incontinence and the number of vaginal deliveries or number of cesarean deliveries was assessed by frequency and Pearson X 2 test using a significance level of less than 0.05. Logistic regression was used to investigate the significance of dichotomous dependent variables. Results: Decades ago, most Taiwanese women (1435 of 1511 respondents, 94.97%,) gave birth via vaginal delivery and the rate of cesarean delivery was low (20 of 1513 respondents, 1.32%). Parity (odds ratio [OR], 2.42; 95% confidence interval [CI], 0.87-6.71; P=0.091), vaginal delivery (OR, 0.76; 95% CI, 0.39-1.47; P=0.408), and cesarean delivery (OR, 1.47; 95% CI, 0.59-3.70; P=0.409) did not increase the risk of urinary incontinence. Conclusion: There was no association between urinary incontinence and parity or mode of delivery among Taiwanese postmenopausal women decades after their first delivery. © 2012 Published by Elsevier Ireland Ltd. on behalf of International Federation of Gynecology and Obstetrics.


Higuchi A.,National Central University | Higuchi A.,National Health Research Institute | Higuchi A.,Cathay Medical Research Institute | Chuang C.-W.,National Central University | And 10 more authors.
Journal of Membrane Science | Year: 2011

Adipose-derived stem cells (ADSCs) were purified from mice adipose-tissue cell solutions by the conventional culture method and the membrane filtration (i.e., batch-type filtration and perfusion-type filtration) method. The ADSCs expressing the mesenchymal stem cell marker CD73 were concentrated in a recovery solution through one sheet of polyurethane (PU) foaming membranes with a pore size of 11 μm, and in a permeate solution through five sheets of Nylon mesh filters with a pore size of 11 μm, by the perfusion-type filtration method. This provided a concentration of cells expressing the marker that was 1.7 times higher than that of cells in the primary adipose-tissue cell solution. The ADSCs in the recovery solution that went through the PU foaming membranes but not through the Nylon mesh filters showed greater adipogenic and osteogenic differentiation ability than the cells contained in the primary adipose-tissue cell solution. The perfusion-type filtration effectively recovered ADSCs with a greater ability to differentiate into adipocytes and osteoblasts than the cells recovered by batch-type filtration. These results suggested that the ADSCs with adipogenic and osteogenic differentiation ability tended to adhere to PU membranes but not to Nylon mesh filters when using perfusion-type filtration. The relationship between the ratio of cells expressing the mesenchymal stem cell surface marker (i.e., CD73) and the adipogenic and osteogenic differentiation ability of the cells was also investigated. © 2010 Elsevier B.V.


Higuchi A.,National Central University | Higuchi A.,King Saud University | Ling Q.-D.,National Central University | Ling Q.-D.,Cathay Medical Research Institute | And 8 more authors.
Laboratory Investigation | Year: 2015

Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs. © 2015 USCAP, Inc All rights reserved.


Higuchi A.,National Health Research Institute | Higuchi A.,National Central University | Higuchi A.,Cathay Medical Research Institute | Huang S.-C.,National Central University | And 8 more authors.
Current Nanoscience | Year: 2011

Stem cells from amniotic fluid were cultured on dishes coated or grafted with extracellular matrix (ECM) or Matrigel where gelatin, collagen, fibronectin, laminin, and vitronectin were selected as ECM components (nanosegments). The effects of interactions between amniotic fluid stem cells and nanosegments were investigated on the expression of surface markers of mesenchymal stem cells and on the differentiation abilities of osteoblasts and neural cells. The ECM-coated dishes produced water contact angles from 35 to 65 degrees, whereas ECM-grafted dishes produced water contact angles from 50 to 70 degrees, which was an adequate water contact angle range for our cell culture conditions. Culture on ECM-immobilized dishes enhances amniotic fluid stem cell differentiation into osteoblasts more than culture on polystyrene dishes grafted with amino groups (PS-NH2 dishes). This finding indicates that specific interactions between amniotic fluid cells and the ECM grafted onto the culture dishes promote the differentiation of cells into osteoblasts. Matrigel-immobilized dishes promoted a more extensive differentiation of amniotic fluid stem cells into neural cells than did ECM- immobilized dishes, but they did not promote differentiation into osteoblasts. Immobilization of the optimal nanosegments (ECM or Ma- trigel) onto culture dishes enhances amniotic fluid stem cell differentiation into osteoblasts and neural cells; the choice of nanosegments depends on the desired differentiated cell type. © 2011 Bentham Science Publishers.


Higuchi A.,National Central University | Higuchi A.,National Health Research Institute | Higuchi A.,Cathay Medical Research Institute | Shen P.-Y.,National Central University | And 8 more authors.
Tissue Engineering - Part A | Year: 2011

The effect of visible light irradiation on the expression of pluripotent genes (Oct-4, Sox2, and Nanog) in amniotic fluid-derived stem cells (AFSCs) and on the osteogenic differentiation ability of AFSCs was investigated using light-emitting diodes (LEDs) at 0-2mW/cm2 in various wavelengths : [blue (470nm), green (525nm), yellow (600nm), and red (630nm)]. Pluripotent gene expression in AFSCs was up-regulated by visible light irradiation from a LED for more than 6h. Green light irradiation of AFSCs up-regulated the expression of pluripotent genes more significantly than irradiation with other light. The osteogenic differentiation of AFSCs was facilitated by green and blue light irradiation. Facilitated differentiation into osteogenic cells by visible light irradiation was not mediated by reactive oxygen species (ROS); alkaline phosphatase activity (a marker of early osteogenic differentiation) and gene expression of osteopontin (a marker of late osteogenic differentiation) did not change significantly between AFSCs in differentiation medium with or without a ROS scavenger (vitamin C). The mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway, as well as other unknown signaling pathways, may be responsible for the activation of signaling pathways that facilitate the differentiation of AFSCs into osteogenic cells on light irradiation. © Copyright 2011, Mary Ann Liebert, Inc. 2011.


PubMed | National Central University, University Putra Malaysia, King Saud University, National Health Research Institute and 3 more.
Type: Journal Article | Journal: Laboratory investigation; a journal of technical methods and pathology | Year: 2014

Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs.


Wang J.,Fu Jen Catholic University | Chiu W.-H.,National Yang Ming University | Chiu W.-H.,Central Clinic and Hospital | Chiu W.-H.,Jinan University | And 7 more authors.
Asia-Pacific Journal of Public Health | Year: 2015

The authors sought to explore the prevalence and factors related to nonalcoholic fatty liver disease (NAFLD) among occupational population in Taipei, Taiwan. A total of 8347 healthy adults voluntarily admitted to annual physical check-up. Blood samples and ultrasound-proved fatty liver sonography results were collected. The results showed that the prevalence of NAFLD was 48.4% and revealed a statistically significant increase with increasing population age. Males exhibited a greater prevalence of NAFLD than did females (57.8% vs 32.4%, P <.001). Using multiple logistic regression analysis, in addition to male gender, older age, higher body mass index, higher aspartate aminotransferase level, higher alanine aminotransferase level, presence of hypertension, presence of hyperuricemia, presence of hypercholesterolemia, higher fasting plasma glucose, and presence of hypertriglyceridemia were the significant factors associated with NAFLD. The differences in occupational professions were revealed. In conclusion, occupational populations are asymptomatic, and the diagnosis of NAFLD should be considered with older age, hyperuricemia, higher aspartate aminotransferase level, higher alanine aminotransferase level, and metabolic risk factors. © 2013 APJPH.


PubMed | MacKay Memorial Hospital, Gene Biodesign Co., Taipei Medical University, National Yang Ming University and 2 more.
Type: Journal Article | Journal: Taiwanese journal of obstetrics & gynecology | Year: 2017

We present prenatal diagnosis of familial transmission of 17q12 duplication associated with no apparent phenotypic abnormality.A 36-year-old woman underwent amniocentesis at 17 weeks of gestation because of advanced maternal age. Cytogenetic analysis revealed a karyotype of 46,XY. Array comparative genomic hybridization of uncultured amniocytes revealed a 1.42-Mb duplication of 17q12 or arr 17q12 (34,822,465-36,243,365) 3 encompassing 12 Online Mendelian Inheritance in Man (OMIM) genes including LHX1, ACACA, and HNF1B. Array comparative genomic hybridization analysis of parental bloods revealed no genomic imbalance in the mother, and a result of arr 17q12 (34,611,377-36,248,889) 2.9 encompassing 16 OMIM genes, including LHX1, ACACA, and HNF1B, in the 29-year-old phenotypically normal father. Prenatal ultrasound findings were unremarkable. The parents elected to continue the pregnancy. At 37 weeks of gestation, a 2789-g normal male baby was delivered uneventfully. When examined at the age of 7 months, the neonate was as phenotypically normal as his father.The 17q12 microduplication may present with variable phenotypes including no apparent phenotypic abnormality in familial cases. However, neuropsychiatry assessment and monitoring should be warranted in childhood and through adulthood under such a circumstance.

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