Hung Kung University

Taichung, Taiwan

Hung Kung University

Taichung, Taiwan
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Yuan S.-Y.,Taichung Veterans General Hospital | Yuan S.-Y.,Hung Kung University | Shiau M.-Y.,Hung Kung University | Ou Y.-C.,Taichung Veterans General Hospital | And 8 more authors.
Oncology Reports | Year: 2017

Miconazole (MIC), an antifungal agent, diplays anti-tumorigenic activity in various types of human cancers, including bladder cancer, yet its mechanism of antitumor action is not well understood. In the present study, we demonstrated that, in a cell viability assay, MIC had a cytotoxic effect on human T24, J82 and TSGH-8301 bladder cancer cells in a dose- and time-dependent manner, but did not exhibit significant toxicity toward human peripheral blood mononuclear cells. Cell cycle analysis revealed that MIC at concentrations of 25 and 50 μM significantly caused G0/G1 arrest in the TSGH-8301 and T24 cells, respectively. DNA fragmentation, mitochondrial membrane potential and western blot analyses showed that MIC inhibited the growth of these cells by both mitochondrial-mediated and death receptor (DR5)-mediated apoptosis pathways. Specifically, MIC increased the protein levels of p21 and p27, but decreased the expression of cyclin E1, CDK2 and CDK4. MIC augmented the expression of DR5, cleaved forms of caspase-3-8 and -9, poly(ADP-ribose) polymerase and Bax, decreased the expression of Bcl-2 but increased cytosol levels of cytochrome c. Our results suggest that MIC inhibits the growth of bladder cancer cells through induction of G0/G1 arrest and apoptosis via activation of both the extrinsic and intrinsic apoptotic pathways. MIC is a potential chemotherapeutic agent for treating bladder cancer in humans.


Lin C.-H.,National Yang Ming University | Lin C.-H.,Hung Kung University | Chou L.-W.,National Yang Ming University | Wei S.-H.,National Yang Ming University | And 3 more authors.
Computer Methods and Programs in Biomedicine | Year: 2014

Background and objective: This study was designed to establish the validity and reliability of a new device that measures bilateral shoulder and elbow range of motion (ROM) and grip force performance in vivo. A further aim was to investigate the control of inter-limb grip force coordination during isometric force-maintenance tasks. Validity of the ROM and grip force measurements was examined using a validated clinical goniometer and standard weights. Subjects: Twenty-one healthy adults (six female, 15 male mean. ±. standard deviation age. = 23.05. ± 3.51) were recruited for this study. Design: All subjects were asked to perform tests to evaluate the validity and reliability of ROM, grip force maximum voluntary contraction (MVC) and coordination control measurements. Results: The ROM and grip force measurements were linearly correlated with criterion standards. For reliability testing, all of the intraclass correlation coefficient values were >0.99. The inter-limb grip force coordination control task showed that the force modulation timing during dominant-to-non-dominant hand transition was longer than the non-dominant-to-dominant hand transition (p< 0.05). Conclusions: These results demonstrate that this device is valid and reliable when used to measure shoulder and elbow ROM and grip force of both hands. Isometric force-maintenance tasks also indicated changes in inter-limb grip force control. © 2014 Elsevier Ireland Ltd.


Wang L.-Y.,National Taiwan University | Wang L.-Y.,National Taiwan University Hospital | Luo H.-J.,National Taiwan University | Luo H.-J.,Hung Kung University | And 8 more authors.
Pediatric Pulmonology | Year: 2010

Oral feeding has been reported to compromise breathing among preterm infants with bronchopulmonary dysplasia (BPD) during hospitalization or shortly after discharge. However, limited information was available concerning whether preterminfants with BPD remain vulnerable to feeding and growth insufficiency after a longer term of follow-up. The purpose of this study was therefore to examine the effect of severity of BPD on pulse oxygen saturation (SpO 2) during feeding and growth in very low birth weight (VLBW) preterm infants during infancy. Seventy-two preterm infants with VLBW and 15 terminfants were prospectively examined their growth and SpO2 during feeding at 2, 4, and 6 months of corrected age. The severity of BPD was graded in VLBW infants according to the American National Institutes of Health consensus definition. In comparison to VLBW infants with mild BPD and terminfants, VLBW infants with severe BPD showed significantly lower mean levels of SpO 2 during feeding at 2-6 months corrected age (P<0.05). Those with severe BPD further exhibited higher rates of growth delay (weight<10th percentile) throughout the study period. Among VLBW infants, severe BPD had an adverse relation with subsequent weight measures after adjustment for medical and demographic confounding variables (β=-904 g, P=0.03). The consensus BPD definition is useful to identify those preterm infants who are at greater risk of feeding desaturation and growth delay during infancy and close monitoring of SpO2 during feeding should be advised. © 2010 Wiley-Liss, Inc.


Yu W.-J.,Hung Kung University | Yu B.-C.,National Cheng Kung University | Yang F.-Y.,National Cheng Kung University | Chang C.-C.,Tainan University of Technology | And 2 more authors.
Journal of Food and Drug Analysis | Year: 2012

One of the major causes of the decline in semen quality appears to be the elevated levels of oxidative stress. Leaves of Toona Sinensis (TSL) have been used as a traditional medicine for treatment of certain male reproduction-related disorders in Chinese ancient medical books; however, the scientific data are limited. In the present study, human spermatozoa treated with hydrogen peroxide (H2O2) were used to investigate the protective function of TSL-6 under oxidative stress. The intracellular reactive oxygen species (ROS) levels of human spermatozoa were increased after 0.2 mmol/L H2O2 treatment and the elevated levels were diminished by TSL-6 dose dependently. The protection of TSL-6 against oxidative stress in human spermatozoa was further substantiated by increasing sperm motility assay including computer assisted sperm analysis (CASA), percentage of high MMP cells, ATP production, percentage of double strand DNA cells, and decreasing cell death. Our results suggested that TSL-6 improved functions of spermatozoa under oxidative stress via decreasing ROS level and cell death. In addition, TSL-6 increased sperm motility, MMP, ATP levels, and maintained the integrity of chromatin structure. TSL-6 is beneficial for human sperm and is potentially developed into functional foods for infertile men under oxidative stress.


Fan H.-J.,Hung Kung University | Lu C.-S.,National Taichung Nursing College | Lee W.-L.W.,Chung Shan Medical University | Chiou M.-R.,Hung Kung University | Chen C.-C.,National Taichung University of Education
Journal of Hazardous Materials | Year: 2011

The Crystal Violet (CV) dye represented one of the major triphenylmethane dyes used in textile-processing and some other industrial processes. Various metals doped titanium dioxide (TiO2) photocatalysts have been studied intensively for the photodegradation of dye in wastewater treatment. In order to understand the mechanistic detail of the metal dosage on the activities enhancement of the TiO2 based photocatalyst, this study investigated the CV photodegradation reactions under UV light irradiation using a Pt modified TiO2 photocatalyst. The results showed that Pt-TiO2 with 5.8% (W/W) Pt dosage yielded optimum photocatalytic activity. Also the effect of pH value on the CV degradation was well assessed for their product distributions. The degradation products and intermediates were separated and characterized by HPLC-ESI-MS and GC-MS techniques. The results indicated that both the N-de-methylation reaction and the oxidative cleavage reaction of conjugated chromophore structure occurred, but with significantly different intermediates distribution implying that Pt doped TiO2 facilitate different degradation pathways compared to the P25-TiO2 system. © 2010.


Huang J.-A.,Taichung Veterans General Hospital | Huang J.-A.,Hung Kung University | Weng R.-H.,Chia Nan University of Pharmacy and Science | Lai C.-S.,National Yunlin University of Science and Technology | Hu J.-S.,Fu Jen Catholic University
Evaluation and the Health Professions | Year: 2013

The purpose of this study was to explore the perceptual market orientation gaps between physician and patients and its impact on relationship quality and patient loyalty, and to verify the critical role of internal marketing on developing market orientation. Self-administered questionnaires were then employed to collect the data. We used a convenience sample of physicians and patients at a medical center in central Taiwan, and 90 usable questionnaires from physicians and 450 usable questionnaires from patients were returned. Hierarchical multiple regression analyses were used to validate the research hypotheses. Physicians' perceptual market orientation had a positive influence on physician-patient relationship quality, hospital-patient relationship quality, and patient loyalty, but the perceptual market orientation gap between physicians and their patients had a direct negative impact on these constructs. Finally, internal marketing was found to have an influence on relationship quality and patient loyalty through the mediation of market orientation. In order to enhance relationship quality and patient loyalty, hospital managers should focus their efforts on improving employees' market orientation and reducing patients' perceptual market orientation gap. In addition, internal marketing was found to be a useful pathway in developing market orientation. © The Author(s) 2012.


Yu C.-S.,China Medical University at Taichung | Huang A.-C.,St. Mary's College | Yang J.-S.,China Medical University at Taichung | Yu C.-C.,China Medical University at Taichung | And 7 more authors.
Anticancer Research | Year: 2012

Safrole, a component of Piper betle inflorescence, is a carcinogen which has been demonstrated to induce apoptosis on human oral cancer HSC-3 cells in vitro and to inhibit HSC- 3 cells in xenograft tumor cells in vivo. In our previous study, safrole promoted phagocytosis by macrophages and natural killer cell cytotoxicity in normal BALB/c mice. The cytotoxic effects of safrole on HL-60 cells were investigated by using flow cytometric analysis, comet assay, 4′,6-diamidino-2-phenylindole (DAPI) staining, western blotting and confocal laser microscopy. The obtained results indicate that safrole induced a cytotoxic response through reducing the percentage of viable cells and induction of apoptosis in HL-60 cells in a dose-dependent manner. DAPI staining and comet assay also showed that safrole induced apoptosis (chromatin condensation) and DNA damage in HL-60 cells. The flow cytometric assay showed that safrole increased the production of reactive oxygen species (ROS) and Ca 2+ and reduced the mitochondrial membrane potential in HL- 60 cells. Safrole enhanced the levels of the pro-apoptotic protein BAX, inhibited those of the anti-apoptotic protein BCL-2 and promoted the levels of apoptosis-inducing factor (AIF) and endonuclease G (Endo G) in HL-60 cells. Furthermore, safrole promoted the expression of glucose-regulated protein 78 (GRP78), growth arrest- and DNA damage-inducible gene 153 (GADD153) and of activating transcription factor 6α (ATF-6α). Based on these findings, we suggest that safrole-induced apoptosis in HL-60 cells is mediated through the ER stress and intrinsic signaling pathways.


Lin C.-H.,National Yang Ming University | Lin C.-H.,Hung Kung University | Chou L.-W.,National Yang Ming University | Wei S.-H.,National Yang Ming University | And 3 more authors.
Experimental Gerontology | Year: 2014

Degeneration in the neuromuscular system due to aging can affect daily activities that need to be controlled by bimanual coordination with both hands. However, little is known about the influence of aging on grip strength and bimanual coordination control between hands. The purpose of this study was to investigate the influence of aging on the maximum grip force output and capacity of coordination control of two hands. Ten healthy elderly and 21 young adults were recruited and asked to execute maximum grip force tests and bimanual coordination control tasks with reciprocal grasping, holding, and releasing of a dynamometer with both hands at three target force levels (10, 20 and 40% maximal voluntary contraction, MVC). Compared with the young group, the maximum grip force of the hands of the elderly group was significantly lower by 77.5% (p. <. 0.05) and 71.1% (p. <. 0.05) in the dominant and non-dominant hands, respectively. The elderly adults also displayed a significantly longer alternating time control in the dominant to non-dominant and non-dominant to dominant hands at the 20% MVC target force level (p. <. 0.05). Aging reduces the maximum hand grip force output and the performance of bimanual coordination control of two hands, which may lead to difficulty with the execution of daily activities requiring both hands. © 2014 Elsevier Inc.


PubMed | Taichung Veterans General Hospital, National Chung Hsing University and Hung Kung University
Type: | Journal: European journal of pharmacology | Year: 2015

Nortriptyline (NTP), an antidepressant, has antitumor effects on some human cancer cells, but its effect on human bladder cancer cells is not known. In this study, we used a cell viability assay to demonstrate that NTP is cytotoxic to human TCCSUP and mouse MBT-2 bladder cancer cells in a concentration and time-dependent manner. We also performed cell cycle analysis, annexin V and mitochondrial membrane potential assays, and Western blot analysis to show that NTP inhibits cell growth in these cells by inducing both mitochondria-mediated and death receptor-mediated apoptosis. Specifically, NTP increases the expression of Fas, FasL, FADD, Bax, Bak, and cleaved forms of caspase-3, caspase-8, caspase-9, and poly(ADP-ribose) polymerase. In addition, NTP decreases the expression of Bcl-2, Bcl-xL, BH3 interacting domain death agonist, X-linked inhibitor of apoptosis protein, and survivin. Furthermore, NTP-induced apoptosis is associated with reactive oxygen species (ROS) production, which can be reduced by antioxidants, such as N-acetyl-L-cysteine. Finally, we showed that NTP suppresses tumor growth in mice inoculated with MBT-2 cells. Collectively, our results suggest that NTP induces both intrinsic and extrinsic apoptosis in human and mouse bladder cancer cells and that it may be a clinically useful chemotherapeutic agent for bladder cancer in humans.


PubMed | Hung Kung University
Type: Journal Article | Journal: Journal of hazardous materials | Year: 2010

The Crystal Violet (CV) dye represented one of the major triphenylmethane dyes used in textile-processing and some other industrial processes. Various metals doped titanium dioxide (TiO(2)) photocatalysts have been studied intensively for the photodegradation of dye in wastewater treatment. In order to understand the mechanistic detail of the metal dosage on the activities enhancement of the TiO(2) based photocatalyst, this study investigated the CV photodegradation reactions under UV light irradiation using a Pt modified TiO(2) photocatalyst. The results showed that Pt-TiO(2) with 5.8% (W/W) Pt dosage yielded optimum photocatalytic activity. Also the effect of pH value on the CV degradation was well assessed for their product distributions. The degradation products and intermediates were separated and characterized by HPLC-ESI-MS and GC-MS techniques. The results indicated that both the N-de-methylation reaction and the oxidative cleavage reaction of conjugated chromophore structure occurred, but with significantly different intermediates distribution implying that Pt doped TiO(2) facilitate different degradation pathways compared to the P25-TiO(2) system.

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