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Dai S.,Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop | Sun X.,Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop | Xiong X.,Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop | Li M.,Hunan Agricultural University | Yao J.,Hunan Agricultural University
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2012

In order to improve the germination rate via mechanical seedcoat nicking method instead of manual operation. The germination hindrance of triploid watermelon seed was analyzed by seedcoat ultrastructure features compared with diploid watermelon seed. Familiar watermelon seeds of three varieties, Heiniu, Mihong and Huapi, at the moisture content of 11.1, 11.2 and 10.6% (w.b.), were selected to study the mechanical nicking properties using common methods, respectively. Test results showed that the loading speed of pressure had an influence on the seedcoat nicking force. The maximum compression values of all seeds were as the designing standard for the compressing rocker. The loading speed of pressure was chosen as 80-100 mm/min; the maximum nicking force as 68.97 N. It is concluded that the results provide basic parameters and theoretical basis to design a seedcoat nicking machine for triploid watermelon seeds.


Huang K.,Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop | Huang K.,Hunan Agricultural University | Lin J.C.,Xiamen University | Wu Q.Y.,Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop | And 6 more authors.
Plant Molecular Biology Reporter | Year: 2015

Broccoli (Brassica oleracea L. var. italica planck) has been highly valued because of its nutrient content, which has been attributed to both sulforaphane, a sulfur-containing compound, and selenium enrichment ability. The sulforaphane synthesis may be affected by Se/S metabolism because sulfate and selenate share the same initial pathway for uptake in plants. Selenocysteine methyltransferase (SMT) plays a critical role in the Se/S metabolism system. In the present work, we analyzed the SMT expression level and sulforaphane content in transgenic broccoli under the conditions of overexpression and RNAi knockdown of SMT under normal and selenium-stressed conditions. The relative expression value of SMT in the overexpression line is 13.93 % higher than that in the untransformed control. Interestingly, the sulforaphane content of the overexpression line was 14.09 % lower after selenium treatment, while that of the empty vector (pCAMBIA1301) transformed and untransformed plants was 59.04 and 66.56 % lower than that of the non-selenium-treated, respectively. In the SMT-RNAi line, which has a relative expression value for SMT 15.60 % lower than that of the untransformed, we cannot detect sulforaphane after selenium treatment. These results showed that SMT plays a key role in sulforaphane synthesis in a selenium-rich environment. Specifically, overexpression of SMT decreases the negative effect of selenium on sulforaphane synthesis, while knockdown of SMT by RNAi enhances the negative effect. © 2015 Springer Science+Business Media New York


Shengping W.,Hunan Agricultural University | Shengping W.,CAS Institute of Subtropical Agriculture | Fang J.,Hunan Agricultural University | Xiangshan T.,CAS Institute of Subtropical Agriculture | And 8 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

NSP is the major factor introducing a low digestibility of cottonseed meal in piglets. The pre study was conducted to determine the effect of NSP enzyme on the nutrient digestibility of weaned piglets a cottonseed meal-based diets. About 15 weaned piglets (14±1.4kg) were selected and ileal fistula surgery done to determine the DE, CP, DM and NSP's apparent digestibility. The results indicated that NSP enz improved piglet's apparent digestibility (p<0.05) in varying degrees; pectinase resulted in obvious degrada of the cell wall and increased piglets' DE (26.69%) andCP of ileal digesta (20.33%) and compound enzyme better in improving cottonseed meal based diets than single enzyme. © Medwell Journals, 2012.


Huang K.,Hunan provincial key laboratory for germplasm innovation and utilization of crop | Huang K.,Hunan Agricultural University | Huang K.,China Agricultural University | Wu Q.,Hunan provincial key laboratory for germplasm innovation and utilization of crop | And 3 more authors.
African Journal of Biotechnology | Year: 2011

The factors which influence the regeneration of broccoli (Brassica oleracea var. italica) were studied using an orthogonal design. The results showed that the major factor was the explant type, followed by naphthylacetic acid (NAA), benzylaminopurine (BAP), sucrose and AgNO3 in turn. The maximum regeneration was on Murashige and Skoog (MS) medium + NAA 0.107 μM + BAP 17.76 μM + 2% sucrose + 0.8% agar. Hypocotyl proved to be the optimum explant source and its regeneration frequency reached 100%. © 2011 Academic Journals.


Wu X.,Hunan Agricultural University | Ren W.,CAS Institute of Subtropical Agriculture | Xu-Gang S.,University of Science and Technology of China | Zheng R.,University of Science and Technology of China | And 3 more authors.
Journal of Food, Agriculture and Environment | Year: 2012

This study was conducted to test the hypothesis that the dietary glutamine supplementation had various effects on oedema disease induced by Escherichia coli (E. coli). Total of 162 KunMing mice were randomly assigned to glutamine group 1 (1.0% glutamine+basal diet, n = 45), glutamine group 2 (1.0% glutamine+basal diet, n = 45), control group 1 (1.22 % alanine+basal diet, n = 36) and control group 2 (1.22% alanine+basal diet, n = 36). After 3 days of adaptive feeding and a 7-days treatment period with the experiment feed, all mice were challenged by intraperitioneal injection of E. coli O 139at LD50 (2.53×10 8 CFU/ml). The mortality after twenty hours of injection was calculated from glutamine group 2 and control group 2. Serum concentrations of platelet-activating factor (PAF), interleukin (IL)-2, interleukin (IL)-10, secretory immunoglobulin A (sIgA), superoxide dismutase (SOD) activity, cyclic 3′,5′-adenosine monophosphate (cAMP) and cyclic 3′,5′-guanosine monophosphate (cGMP) were measured in glutamine group 1 and control group 1 in a ten-hours interval for three times. Serum concentrations of PAF in 1.0% glutamine group were significantly lower (P<0.01) than that in 1.22% alanine group at ten and thirty hours after initial injection and lower (P<0.05) than the control group twenty hours after first injection. Additionally, the supplementation of glutamine increased the SOD activity significantly (P<0.05) in the first twenty hours after initial injection. However, the SOD activity in the glutamine group was lower (P<0.05) than in the control group later. Furthermore serum concentration of cAMP had no difference at the first ten hours after injection, whereas the cAMP concentrations in the glutamine group was significantly lower (P<0.01) at twenty hours after initial injection and lower (P<0.05) than in the control group thirty hours after first injection. Meanwhile, glutamine supplementation had little effect on the mortality of mice, serum IL-2, sIgA and cGMP. In conclusion, dietary glutamine supplementation could partially attenuate the damage caused by oedema disease, but had little effect on the clinical result.


Ren W.,CAS Institute of Subtropical Agriculture | Yin J.,CAS Institute of Subtropical Agriculture | Wu M.,CAS Institute of Subtropical Agriculture | Liu G.,CAS Institute of Subtropical Agriculture | And 10 more authors.
PLoS ONE | Year: 2014

This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)- myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-α) in colitis model. These results were associated with colonic NF-κB, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases. © 2014 Ren et al.


PubMed | Washington State University, Texas A&M University, CAS Institute of Subtropical Agriculture, Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop and Northwest Agriculture and Forestry University
Type: Journal Article | Journal: PloS one | Year: 2014

This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1), IL-6, IL-17 and tumor necrosis factor alpha (TNF-)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-B), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)-myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-) in colitis model. These results were associated with colonic NF-B, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases.

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