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He S.,China Agricultural University | Chen Q.,Hunan Provincial Institute of Animal Drug and Feed Supervision | Sun Y.,China Agricultural University | Zhu Y.,China Agricultural University | And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2011

Pharmaceuticals residues in the environment have become a growing scientific interest worldwide. In the light of the possible harmful effects of tobramycin, a rapid and sensitive analytical method for determination of tobramycin in soil was developed. The extraction and purification methods, derivatization conditions, and chromatographic conditions in the determination of tobramycin in soil have been fully investigated. Extraction was carried out by a combination of vortex mixer and ultrasonic oscillation using acetone/water as the extraction agent. The extract was concentrated to 1mL and passed through the C18 SPE cartridge rinsed with water (3mL), methanol (3mL). The derivatization procedure was followed by the reaction of tobramycin with 4-Chloro-3,5-dinitrobenzotrifluoride at 60°C for 10min in pH 9.0 H3BO3-Na2B4O7 medium. The labeled tobramycin was determined by high performance liquid chromatography at 245nm. Separation was accomplished within 15min in gradient elution mode with trifluoroacetic acid in mobile phase as ion-pair reagent. The correlation coefficient for the method was 0.9999 in concentrations ranging from 0.10 to 100.0μg/g. The limit of detection was 0.02μg/g for tobramycin in soil at a signal-to-noise ratio of 3. The calculated recoveries of the proposed method were from 78.0 to 91.0% and RSDs were 3.38-9.79% in the application to the quantitative determination of tobramycin in all types of soil. The method will help to establish adequate monitoring of tobramycin residue in soil and make the contribution to environmental behavior evaluation. © 2011 Elsevier B.V.

Kuang G.,Hunan Agricultural University | Kuang G.,Hunan Provincial Institute of Animal Drug and Feed Supervision | Xiao A.,Hunan Provincial Institute of Animal Drug and Feed Supervision | Chen Q.,Hunan Provincial Institute of Animal Drug and Feed Supervision | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

A Gram-negative, rod-shaped bacterial strain named PB-CSOl which is bioluminescence-positive was isolated from contaminated commercial pork that probably had been exposed to seafood. Optimal growth of strain PB-CSOl requires the presence of 3.0% (w/v) NaCl and a temperature of 20°C. Phylogenetic analysis based on 16S rKMA gene sequences of strain PB-CSOl and other Photobacterium species showed that the novel isolate belongs to the genus Photobacterium. Sequence similarity analysis between PB-CSOl and other species indicates that the closest relatives of strain PB-CSOl are Photobacterium phosphoreum ATCC 11040 (99.9%), Photobacterium KsMamV pjapo.l. 1 (99.8%) andPhotobacterium iliopiscarium ATCC 51760 (99.5%). The most abundant fatty acids were summed feature 3 (50.77%; C16:1w7c and/or C16:1w6c) and C16:0 (15%). The fatty acid profile is similar to that of the genus Photobacterium but this report is the first to describe C16:1w6c as one of the compositions of summed feature 3 of the genus Photobacterium. The G+C content of the genomicDNA of strain PB-CSOl was 44.8 mol%. Overall, strain PB-CSOl is a novel Photobacterium species. © Medwell Journals, 2012.

Liu Z.-Y.,Hunan Agricultural University | Yang K.,Hunan Agricultural University | Chen F.-H.,Hunan Provincial Institute of Animal Drug and Feed Supervision | Long X.-M.,Hunan Provincial Institute of Animal Drug and Feed Supervision | And 3 more authors.
Food Analytical Methods | Year: 2014

Flunixin is approved for use in veterinary medicine as a nonsteroidal anti-inflammatory agent. There is a need for the control of residues and development of methods to monitor its compliance with legislation. However, few methods have been reported for the analysis of flunixin in the animal liver, kidney, and fat. Therefore, the aim of this work was to develop a relatively rapid sample preparation and sensitive using liquid chromatography–tandem mass (LC-MS/MS) confirmatory method to detect flunixin residues in the liver, kidney, muscle, and fat of swine and chicken. After acid hydrolysis, the sample was extracted with ethyl acetate. The extract was finally evaporated to dryness and reconstituted in a water/methanol mixture, and determination was carried out by LC-MS/MS. Flunixin was detected using positive electrospray ionization in selected reaction monitoring (SRM). Estimated limit of quantification of the method was 0.5, 0.5, 0.25, and 0.05 μg/kg for the liver, kidney, muscle, and fat, respectively. The method was validated in animal tissues in terms of selectivity, linearity, trueness, precision, decision limit (CCα), and detection capability (CCβ). All the trueness values fell within a range between 73.6 and 84.8 %. Precision values for all levels of concentration tested showed excellent relative standard deviation (RSD < 15 %). The CCα and CCβ values have been established for each tissue. A relatively rapid and sensitive LC-MS/MS method for the quantitative determination of flunixin in different animal tissues was developed and validated. The method is suitable for monitoring the flunixin residues in animal tissues. © 2014, Springer Science+Business Media New York.

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