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Liu Y.,University of South China | Liu Y.,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study | Yang S.,Hunan University | Xiao J.,University of South China | And 7 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2015

The present study was to determine the targeting effect of M13 phage peptide ZL4 (MppZL4) on Schistosoma japonicum (S.j). Mice infected with S.j were injected with MppZL4. Real-time PCR was used to detect the distribution and metabolism of MppZL4 in the livers and lungs of mice. In vivo refusion test was performed to detect the targeting of MppZL4. Western blotting was employed to determine the expression of MppZL4. Live imaging was used to detect the distribution of oligopeptide MppZL4. Immunohistochemistry was employed to determine MppZL4 location on adult S.j body surface. Gomori method was employed to detect the influence of oligopeptide MppZL4 on alkaline phosphatase activity. The distribution and metabolism of MppZL4 and M13KE are not significantly different from each other at each time point. The abundance of MppZL4 is changed as S.j migrates in mice. The targeted binding effect of MppZL4 varies at different stages. ZL4 oligopeptide targets S.j in mice. The specific binding sites of MppZL4 on S.j body are mainly located in syncytial cells. The binding sites of MppZL4 on S.j body surface might be ALP or ALP-related proteins. MppZL4 had targeted binding effect on S.j with its binding site being associated with proteins related to S.j alkaline phosphatase. S.j tegument had a specifically binding site with exogenous peptides, offering new means to explore the interactions between hosts and parasites. Additionally, MppZL4 can possibly be used as targeting molecules in worm-resistant drugs or as tracing molecules in imaging diagnosis technologies. Source


Bo S.,University of South China | Bo S.,Wuhan University | Bo S.,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study | Song C.,Wuhan University | And 9 more authors.
Journal of Organic Chemistry | Year: 2015

Both 19F MRI and optical imaging are powerful noninvasive molecular imaging modalities in biomedical applications. To integrate these two complementary imaging modalities, the design and synthesis of a novel 19F MRI/fluorescence dual-modal imaging agent is reported herein. Through Sonogashira coupling reaction between the fluorinated phenylacetylene and 1,2,4,5-tetraiodobenzene, a fluorophore with 48 symmetrical fluorines at its periphery was constructed with high efficacy. High aqueous solubility was achieved by PEGylation of the fluorophore with monodisperse PEGs. However, an unexpected self-assembly of the PEGylated amphiphilic fluorophore in water "turned off" the 19F NMR signal. However, hydrogenation of the triple bonds or introduction of branched monodisperse PEGs was able to efficiently tune the self-assembly, resulting in the "turning on" of the 19F NMR signal. One of these amphiphiles combines the advantages of label-free fluorescence, high 19F MRI sensitivity, biocompatibility, and excellent aqueous solubility. The results demonstrate the great potential of such amphiphiles for real-time 19F MRI and fluorescence dual-modality imaging. © 2015 American Chemical Society. Source


Xie Z.-Z.,University of South China | Xie Z.-Z.,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study | Liu Y.,University of South China | Liu Y.,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study | Bian J.-S.,National University of Singapore
Oxidative Medicine and Cellular Longevity | Year: 2016

Intracellular redox imbalance is mainly caused by overproduction of reactive oxygen species (ROS) or weakness of the natural antioxidant defense system. It is involved in the pathophysiology of a wide array of human diseases. Hydrogen sulfide (H2S) is now recognized as the third "gasotransmitters" and proved to exert a wide range of physiological and cytoprotective functions in the biological systems. Among these functions, the role of H2S in oxidative stress has been one of the main focuses over years. However, the underlying mechanisms for the antioxidant effect of H2S are still poorly comprehended. This review presents an overview of the current understanding of H2S specially focusing on the new understanding and mechanisms of the antioxidant effects of H2S based on recent reports. Both inhibition of ROS generation and stimulation of antioxidants are discussed. H2S-induced S-sulfhydration of key proteins (e.g., p66Shc and Keap1) is also one of the focuses of this review. © 2016 Zhi-Zhong Xie et al. Source


Yu W.,Wuhan University | Yang Y.,CAS Wuhan Institute of Physics and Mathematics | Bo S.,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study | Bo S.,University of South China | And 8 more authors.
Journal of Organic Chemistry | Year: 2015

To achieve high sensitivity for 19F MRI, a class of novel dendritic molecules with multiple pseudosymmetrical fluorines was designed and efficiently synthesized. Through iterative bromination and Williamson ether synthesis under mild conditions, a fluorinated dendrimer with 540 pseudosymmetrical fluorines was conveniently prepared without performing the group protection in a convergent way. The dendrimer is characterized by a strong 19F NMR peak and short relaxation times. Eventually, an appreciably enhanced 19F MRI at an extremely low concentration (18.5 μM) was achieved, which demonstrated the potential utility of such dendritic molecules in highly sensitive 19F MRI. (Chemical Equation Presented). © 2015 American Chemical Society. Source


Xia G.,Wuhan University | Li Y.,Wuhan University | Yang Z.,Wuhan University | Yang Z.,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study | And 3 more authors.
Organic Process Research and Development | Year: 2015

We have developed a process for the efficient and scalable preparation of heterofunctionalized dodecaethylene glycol from the readily available tetraethylene glycol and its macrocyclic sulfate. By employing the benzyl group as both a protecting group and a separative tag, multiple chromatographic separations were avoided. With this method, α-amino-ω-methyl-dodecaethylene glycol was prepared on a 53 g scale with high purity and 61% overall yield in eight steps and one chromatographic separation. © 2015 American Chemical Society. Source

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