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PubMed | Hunan Plant Protection Institute, Tianjin University, China Offshore Environmental Service Co., Tianjin University of Science and Technology and Shenzhen Key Laboratory of Environmental Microbial Genomics and Application
Type: Journal Article | Journal: Genome announcements | Year: 2014

Brachybacterium phenoliresistens strain W13A50 was isolated from a petroleum-contaminated saline site, which could degrade hydrocarbon under high salinity conditions. Here, we present 4.2-Mb draft genome sequence of this strain, which will provide insights into the diversity of Brachybacterium and the mechanism of hydrocarbon degradation in saline environments.


PubMed | Yangtze University, Hunan Plant Protection Institute and Fujian Agriculture and forestry University
Type: | Journal: Archives of virology | Year: 2016

A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named Brassica campestris chrysovirus 1 (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respectively, each containing a single open reading frame (ORF 1-3). The putative proteins encoded by ORF 1-3 show homologies to RdRp, CP and chryso-P3 of approved or tentative chrysoviruses. In addition, the three dsRNAs of BrcCV1 contain highly conserved 5 and 3 untranslated regions (UTRs) in a way similar to known chrysoviruses. In a phylogenetic tree based on the conserved amino acid sequences of the RdRps of chrysoviruses, totiviruses and partitiviruses, the putative BrcCV1 formed a separate clade with Raphanus sativus chrysovirus 1 (RasCV1), a putative trisegmented, plant-infecting chrysovirus, in the family Chrysoviridae.


PubMed | Hunan Plant Protection Institute, CAS Research Center for Eco Environmental Sciences, Tianjin University and Shenzhen Key Laboratory of Environmental Microbial Genomics and Application
Type: Journal Article | Journal: Genome announcements | Year: 2015

Ochrobactrum anthropi W13P3 was isolated from saline soil contaminated by polycyclic aromatic hydrocarbons (PAHs) and could degrade PAHs with 5% NaCl. We report the 5.3-Mb draft genome sequence of this strain, which is helpful for understanding the diversity of Ochrobactrum spp. and the mechanism of PAH degradation in saline environments.


PubMed | Hunan Plant Protection Institute, CAS Research Center for Eco Environmental Sciences, Tianjin University and Shenzhen Key Laboratory of Environmental Microbial Genomics and Application
Type: Journal Article | Journal: Genome announcements | Year: 2015

Aquamicrobium defluvii W13Z1 was isolated from petroleum-contaminated drill cuttings from the Bohai Sea and could degrade petroleum hydrocarbon with 5% NaCl at 15C. Here, we present the 4.8-Mb draft genome sequence of this strain, which may provide useful information about the mechanism of petroleum degradation in drill cuttings.


PubMed | Hunan Plant Protection Institute, CAS Research Center for Eco Environmental Sciences, Tianjin University and Shenzhen Key Laboratory of Environmental Microbial Genomics and Application
Type: Journal Article | Journal: Genome announcements | Year: 2016

Pannonibacter phragmitetus CGMCC9175 is a halotolerant polycyclic aromatic hydrocarbon (PAH)-degrading bacterium isolated from PAH-contaminated intertidal zone sediment. Here, we report the 5.7-Mb draft genome sequence of this strain, which will provide insights into the diversity of Pannonibacter and the mechanism of PAH degradation in sediments.


Zhang D.,Hunan Plant Protection Institute | Tan X.,Hunan Plant Protection Institute | Willingmann P.,University of Hamburg | Adam G.,University of Hamburg | Heinze C.,University of Hamburg
Journal of Phytopathology | Year: 2011

Among the Chili breeding lines from the Asian Vegetable Research Center, two were chosen for the screening of a larger selection of Cucumber mosaic virus (CMV) isolates, mainly from Asian countries. The chili line (VC246) showed a resistance against several CMV-isolates and was compared with chili line VC27a that was susceptible to CMV infection. Among the 28 CMV isolates, five were identified as resistance breaking (AN-like) and non-resistance breaking (P3613-like) for the line VC246, whereas all isolates could establish a systemic infection on VC27a. However, further testing revealed that resistance in VC246 was also dependent on the way of inoculation and the inoculums itself. Graft inoculation could overcome the resistance, and the inoculation with isolated viral RNA resulted in no infection at all on the resistant chili line, independent of the virus isolate. Using a pseudo-recombinant approach, we identified RNA2 of resistance breaking isolates as responsible for systemic infection and confined the area within RNA2 to the 3' terminal part including the ORF 2b. Sequence alignments of that area revealed eight distinct mutations on amino acid level, which was present either in resistance or non-resistance breaking isolates. A reversion from the P3613-like to the AN-like sequence of two of these mutations induced no effect on Capsicum sp., but induced symptoms on several tobacco species distinct from those induced by the wild-type virus. However, pseudorecombinants, each generated from sets of two different AN-like isolates, which were expected to infect VC246 systemically, did not indicating that probably RNA2 must be in a specific context to have the effect. In this case, a generalized attribution of functions to single amino acid exchanges might be impossible or at least extremely difficult. © 2011 Blackwell Verlag GmbH.


PubMed | Hunan Plant Protection Institute, Chinese Academy of Agricultural Sciences and Zhejiang University
Type: | Journal: Virology journal | Year: 2015

Barley yellow dwarf virus (BYDV) is one of the most devastating plant viruses and belongs to a ubiquitous plant virus group. In China, four BYDV strains (GPV, GAV, PAV and RMV) have been identified based on their specific aphid vectors and serological properties. Among the four identified strains, the GAV is the most common BYDV strain in China. To diagnose, forecast of BYDV GAV, two reliable serological assays for BYDV GAV detection were established.We purified virion from a confirmed BYDV GAV source and used it as the immunogen to produce monoclonal antibodies against the virus. Using the hybridoma technology, three highly specific murine monoclonal antibodies were produced and two serological assays [antigen-coated-plate enzyme-linked immunosorbent assay (ACP-ELISA) and dot enzyme-linked immunosorbent assay (dot-ELISA)] were established for the BYDV GAV detection.All three monoclonal antibodies reacted strongly and specifically with the BYDV GAV strain in crude leaf extracts. Titers of the monoclonal antibodies in ascitic fluids were up to 10(-7) by indirect-ELISA. These three monoclonal antibodies (18A1, 18A9 and 12A11) all belonged to the isotype IgG1, kappa light chain. The highest dilution points for the three antibodies during the ACP-ELISA using infected crude leaf extracts were 1:163,840, 1:81,920 and 1:81,920 (w/v, gmL(-1)), respectively. Result of dot-ELISA showed a successful detection of BYDV GAV strain in 1:5,120 (w/v, gmL(-1)) diluted wheat leaf crude extracts. Analysis of 22 field wheat leaf samples and 33 aphid samples from the Shaanxi Province in China, using the two newly developed assays confirmed the presence of BYDV GAV in about 80% of the wheat samples and 18% of the aphid samples.All three monoclonal antibodies are highly sensitive and specific to the BYDV GAV. The two newly developed serological assays are simple and effective. These two assays, particularly the dot-ELISA, are useful for high throughput detection of BYDV GAV in host plants and aphid vectors.


Yin L.,Central South University | Liu Y.,Central South University | Liu Y.,Hunan Plant Protection Institute | Zhang D.,Central South University | And 2 more authors.
Advanced Materials Research | Year: 2012

A bacterial strain S9-1 capable of degrading sulfonylurea herbicide pyrazosulfuron-ethyl (PSE) was isolated from contaminated soil through the enrichment incubation method. Based on morphology, colony and cultural properties, physiological and biochemical characteristics, living-cell absorption spectra, internal photosynthetic membrane, and phylogenetics of its 16S rRNA gene sequence, S9-1 was preliminarily identified as belonging to the genus Rhodopseudomonas, a group of photosynthetic bacteria (PSB). The effects of PSE concentration, pH, and temperature on biodegradation were examined. The degradation rate was found to decrease with increasing PSE concentration. Optimal growth pH and temperature were found to be 7.0 and 30°C, respectively. The strain was able to degrade 47.51% of PSE at a concentration of 100 mg ml-1 after 7 days of incubation at 30°C and could tolerate 800 mg ml-1 PSE. S9-1 was also able to completely co-metabolically transform 100 mg ml-1 PSE at 30°C, pH 7.0, and 7500 lux in 15 days. As the concentration of PSE increased, the degradation process took longer to complete. The fragment encoding acetolactate synthase (ALS) gene from S9-1 was cloned and sequenced. Comparison of deduced amino acid sequences was implemented, and the conserved sites were analyzed. To our knowledge, this is the first report of PSB in PSE biodegradation. These results highlight the potential of this bacterium as a detoxifying agent for use with PSE-contaminated soil and wastewater. © (2012) Trans Tech Publications, Switzerland.


Zhang S.,Central South University | Zhang S.,Hunan Plant Protection Institute | Yin L.,Central South University | Liu Y.,Central South University | And 7 more authors.
Biodegradation | Year: 2011

A novel bacterial strain capable of degrading the pyrethroid pesticide fenpropathrin was isolated from mixed wastewater and sludge samples. Phylogenetic analysis of the 16S rDNA sequence revealed that the organism belongs to the genus Clostridium. The organism can co-metabolically transform fenpropathrin at 100 mg l-1 at 35°C and pH 7.5 in 12 days. Metabolic products of fenpropathrin from strain ZP3 were examined by gas chromatography/mass spectrometry, and the results showed that the organism degraded fenpropathrin with an oxidization process to yield benzyl alcohol, benzenemethanol, 3,5-dimethylamphetamine. Analyses of cell-free extracts from this strain showed that the optimal degrading conditions for degrading fenpropathrin were 35°C and pH 7.5, and degradation efficiency was 20.0 mg l-1 day-1, and it might be potential using for rapid treating fenpropathrin, for example, on the surface of fruits and vegetables. © 2010 Springer Science+Business Media B.V.


Yin L.B.,Shaoyang University | Zhao L.Z.,Shaoyang University | Liu Y.,Hunan Plant Protection Institute | Zhang D.Y.,Hunan Plant Protection Institute | And 2 more authors.
Advanced Materials Research | Year: 2014

A bacterial strain S5-1 capable of degrading chlorimuron-ethyl was isolated from paddy soil using enrichment technique. On the basis of traditional culture characteristics, colony and cell morphology, physiological and biochemical characteristics, type of internal photosynthetic membrane and combined with 16S rRNA gene sequence analysis, the strain was identified as a Rhodopseudomonas sp. The optimal temperature and pH for biodegradation of chlorimuron-ethyl by Rhodopseudomonas sp. strain S5-1 were 35°C and pH 7.0, and the degradation rate reached 87.8% within 10 days under the optimal conditions. The results revealed that strain S5-1 could degrade chlorimuron-ethyl efficiently and for further study it could potentially be used as a biological agent for the remediation of soil, water or crops, contaminated by chlorimuron-ethyl herbicide. © (2014) Trans Tech Publications, Switzerland.

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