Entity

Time filter

Source Type

Changsha, China

Tang Y.,CAS Institute of Subtropical Agriculture | Tan B.,CAS Institute of Subtropical Agriculture | Xiong X.,CAS Institute of Subtropical Agriculture | Li F.,CAS Institute of Subtropical Agriculture | And 5 more authors.
Amino Acids | Year: 2015

Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without l-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy. © 2014 Springer-Verlag Wien. Source


Xiong X.,Observation and Experiment Station of Animal Nutrition and Feed Science in South Central China | Xiong X.,CAS Institute of Subtropical Agriculture | Yang H.S.,Observation and Experiment Station of Animal Nutrition and Feed Science in South Central China | Yang H.S.,University of Chinese Academy of Sciences | And 10 more authors.
Livestock Science | Year: 2014

The objective of the present experiment was to evaluate the effects of dietary supplementation with antimicrobial peptides (AMPs) on growth performance, diarrhea incidence, and survival rate of weaned pigs reared at 5 commercial farms (Farms A-E). Pigs (Farm A, n=486, genotype=Yorkshire (Y)×Landrace (L), weaning age=28±3. d; Farm B, n=360, genotype=Y×L×Duroc (D), weaning age=30±2. d; Farm C, n=558, genotype=Y×L×D, weaning age=30±2. d; Farm D, n=828, genotype=Y×L×D, weaning age=32±3. d; Farm E, n=576, genotype=Y, weaning age=24±3. d) were blocked (within farm) by body weight (BW) and sex and randomly assigned to 1 of 3 treatments: (1) a basal diet (control); (2) 2.0. g/kg of AMPs (AMPs-2) and (3) 3.0. g/kg of AMPs (AMPs-3). The AMPs used in the present study were a mixture of lactoferrin, cecropin, defensin, and plectasin. Each treatment group consisted of 6 replicates per farm. Each replicate consisted of 2 pens of 13-14 pigs, 2 pens of 10 pigs, 3 pens of 10-11 pigs, 4 pens of 11-12 pigs and 3 pens of 10-11 pigs on Farms A, B, C, D, and E respectively. Dietary supplementation with AMPs enhanced (linear, P<0.05; quadratic, P<0.05) average daily gain (ADG) of weaned pigs on all 5 farms. The average daily feed intake (ADFI) was increased (quadratic, P<0.05) by dietary supplementation with AMPs. The feed conversion rate (G:F) was also enhanced (linear, P<0.05) when AMPs were included in the diets of weaned pigs on commercial farms. The diarrhea incidence of AMPs-supplemented pigs was less (P<0.001) than that in control pigs. The survival rate of pigs fed AMPs-supplemented diet was higher (P<0.01) than that of control pigs. The present results indicated that AMPs had beneficial effects on growth performance, reduced the incidence of diarrhea, and increased the survival rate of weaned pigs. © 2014 Elsevier B.V. Source


Chen Q.,Hunan Agricultural University | Ding Z.,Hunan Agricultural University | Qiu W.,Hunan New Wellful Co. | He J.-H.,Hunan Agricultural University
Journal of Animal and Veterinary Advances | Year: 2014

This study was conducted to investigate the effect of Achyranthes Bidentata Polysaccharide (ABP) on growth performance, inflammatory responses and hormones in weaned piglets after Escherichia coli Lipopolysaccharide (LPS) challenges. A total of 48 crossbred (Duroc x Large; White x Landrace) male pigs weaned at 28 days of age (8.45±0.14 kg) were randomly allotted to one of four treatments by initial BW in a 2×2 factorial design that included a dietary addition of ABP (0 or 500 mg kg-1) and immunological challenge (with LPS or saline). On day 14 and 21, pigs were injected intraperitoneally with either 100 μg kg-1 BW of LPS or an equivalent amount of sterile saline. Blood samples were obtained 3 h after injection for analysis of Tumor Necrosis Factor-α (TNF-α), Prostaglandin E2(PGE2), cortisol, Growth Hormone (GH) and Insulin-like Growth Factor (IGF)-I. The results showed that LPS challenge decreased Average Daily Feed Intake (ADFI) (p<0.05) from 14-21 and day 21 "28. ABP increased ADG (p<0.05) and ADFI (p<0.05) from day 21 "28. An interaction (p<0.05) between LPS challenge and diet was observed for the plasma concentration of TNF-α, PGE2, cortisol, and IGF-I after both LPS challenges such that among LPS-treated pigs, pigs fed the ABP diet were lower for TNF-α (p<0.05) and higher IGF-I (p<0.05) than those receiving the control diet. These results indicated that ABP alters the release of inflammatoiy cytokines that may lead to improved pig performance during an immunological stress. © Medwell Journals, 2014. Source


Zhang Y.,CAS Institute of Subtropical Agriculture | Zhang Y.,Hunan Engineering and Research Center Animal and Poultry Science | Feng Z.,CAS Institute of Subtropical Agriculture | Feng Z.,Hunan Engineering and Research Center Animal and Poultry Science | And 5 more authors.
Journal of Animal and Veterinary Advances | Year: 2014

Iatric obesity is a growing problem around the world with an increasing prevalence among even infants and young children. The intestinal microbiota have recently been shown to affect the energy balance by influencing both the efficiency of calorie harvest from the diet and how harvested energy is used and stored and is a non-negligible contributor of the formation of obesity. Pig is a good model for use in human nutrition research but few studies have examined the differences among different pig breeds. This study was conducted to compare the diversities of intestinal microbiota among lean Landrace pig and four local Chinese pig breeds with obese phenotype including Bama mini-pig, Huanjiang mini-pig, Ningxiang pig and Lantang pig. These local Chinese pig breeds have obvious differences in genotype and phenotype compared to Landrace pig. The aim of this study was to examine the association between the intestinal microbiota composition in infancy and future obesity using pigs as a model. The results indicated that the local Chinese pig breeds had significantly greater microbiota diversities in the distal intestine than Landrace pig. There are also clear differences in genus that can influence the energy balance and contribute to obesity. The results indicated that the microbiota diversity in the distal intestine in LCBs was significant higher than in LD which challenge the result that the microbiota diversity decrease in obese individuals. Intestinal microbiota do contribute for the obese phenotype but genotype is the main contributor. The higher contents of intestinal microbial populations that show strong energy-harvesting ability may partly contribute to the obese phenotype in local Chinese pigs. The research may help to clarify the mechanism of phenotype diversity in pig breeds and contribute to studies on infant nutrition and obesity. © Medwell Journals, 2014. Source


Ruan Z.,Nanchang University | Yang Y.,Nanchang University | Zhou Y.,Nanchang University | Wen Y.,Nanchang University | And 10 more authors.
Amino Acids | Year: 2014

This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution 1H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo. © 2014 Springer-Verlag. Source

Discover hidden collaborations