Hunan New Wellful Co.

Changsha, China

Hunan New Wellful Co.

Changsha, China

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Duan J.,Chinese Academy of Sciences | Yin J.,Chinese Academy of Sciences | Wu M.,Chinese Academy of Sciences | Liao P.,Chinese Academy of Sciences | And 16 more authors.
PLoS ONE | Year: 2014

The purpose of this study was to investigate the hypothesis that dietary supplementation with glutamic acid has beneficial effects on growth performance, antioxidant system, intestinal morphology, serum amino acid profile and the gene expression of intestinal amino acid transporters in growing swine fed mold-contaminated feed. Fifteen pigs (LandracexLarge White) with a mean body weight (BW) of 55 kg were randomly divided into control group (basal feed), mycotoxin group (contaminated feed) and glutamate group (2% glutamate+contaminated feed). Compared with control group, mold-contaminated feed decreased average daily gain (ADG) and increased feed conversion rate (FCR). Meanwhile, fed mold-contaminated feed impaired anti-oxidative system and intestinal morphology, as well as modified the serum amino acid profile in growing pigs. However, supplementation with glutamate exhibited potential positive effects on growth performance of pigs fed mold-contaminated feed, ameliorated the imbalance antioxidant system and abnormalities of intestinal structure caused by mycotoxins. In addition, dietary glutamate supplementation to some extent restored changed serum amino acid profile caused by mold-contaminated feed. In conclusion, glutamic acid may be act as a nutritional regulating factor to ameliorate the adverse effects induced by mycotoxins.


Xiong X.,Observation and Experiment Station of Animal Nutrition and Feed Science in South Central China | Xiong X.,CAS Institute of Subtropical Agriculture | Yang H.S.,Observation and Experiment Station of Animal Nutrition and Feed Science in South Central China | Yang H.S.,University of Chinese Academy of Sciences | And 10 more authors.
Livestock Science | Year: 2014

The objective of the present experiment was to evaluate the effects of dietary supplementation with antimicrobial peptides (AMPs) on growth performance, diarrhea incidence, and survival rate of weaned pigs reared at 5 commercial farms (Farms A-E). Pigs (Farm A, n=486, genotype=Yorkshire (Y)×Landrace (L), weaning age=28±3. d; Farm B, n=360, genotype=Y×L×Duroc (D), weaning age=30±2. d; Farm C, n=558, genotype=Y×L×D, weaning age=30±2. d; Farm D, n=828, genotype=Y×L×D, weaning age=32±3. d; Farm E, n=576, genotype=Y, weaning age=24±3. d) were blocked (within farm) by body weight (BW) and sex and randomly assigned to 1 of 3 treatments: (1) a basal diet (control); (2) 2.0. g/kg of AMPs (AMPs-2) and (3) 3.0. g/kg of AMPs (AMPs-3). The AMPs used in the present study were a mixture of lactoferrin, cecropin, defensin, and plectasin. Each treatment group consisted of 6 replicates per farm. Each replicate consisted of 2 pens of 13-14 pigs, 2 pens of 10 pigs, 3 pens of 10-11 pigs, 4 pens of 11-12 pigs and 3 pens of 10-11 pigs on Farms A, B, C, D, and E respectively. Dietary supplementation with AMPs enhanced (linear, P<0.05; quadratic, P<0.05) average daily gain (ADG) of weaned pigs on all 5 farms. The average daily feed intake (ADFI) was increased (quadratic, P<0.05) by dietary supplementation with AMPs. The feed conversion rate (G:F) was also enhanced (linear, P<0.05) when AMPs were included in the diets of weaned pigs on commercial farms. The diarrhea incidence of AMPs-supplemented pigs was less (P<0.001) than that in control pigs. The survival rate of pigs fed AMPs-supplemented diet was higher (P<0.01) than that of control pigs. The present results indicated that AMPs had beneficial effects on growth performance, reduced the incidence of diarrhea, and increased the survival rate of weaned pigs. © 2014 Elsevier B.V.


Xiao D.,Hunan Agricultural University | Wang Y.,Twins Group Co. | Liu G.,CAS Institute of Subtropical Agriculture | He J.,Hunan Agricultural University | And 8 more authors.
PLoS ONE | Year: 2014

The aim of this study was to investigate whether supplementation with chitosan (COS) could reduce diarrhea and to explore how COS alleviates intestinal inflammation in weaned pigs. Thirty pigs (DurocxLandracexYorkshire, initial BW of 5.65±0.27) weaned at age 21 d were challenged with enterotoxigenic Escherichia coli during a preliminary trial period, and then divided into three treatment groups. Pigs in individual pens were fed a corn-soybean meal diet, that contained either 0 (control), 50 mg/kg chlortetracycline, or 300 mg/kg COS for 21 days. The post-weaning diarrhea frequency, calprotectin levels and TLR4 protein expression were decreased (P<0.05) in both the COS and chlortetracycline groups compared with control. Simultaneously, supplemental COS and chlortetracycline had no effect on the mRNA expression of TNF-α in the jejunal mucosa, or on the concentrations of IL-1β, IL-6 and TNF-α in serum. However, COS supplementation improved (P<0.05) the mRNA expression of IL-1β and IL-6 in the jejunal mucosa. The results indicate that supplementation with COS at 300 mg/kg was effective for alleviating intestinal inflammation and enhancing the cell-mediated immune response. As feed additives, chitosan and chlortetracycline may influence different mechanisms for alleviating inflammation in piglets. © 2014 Xiao et al.


Chen Q.,Hunan Agricultural University | Ding Z.,Hunan Agricultural University | Qiu W.,Hunan New Wellful Co. | He J.-H.,Hunan Agricultural University
Journal of Animal and Veterinary Advances | Year: 2014

This study was conducted to investigate the effect of Achyranthes Bidentata Polysaccharide (ABP) on growth performance, inflammatory responses and hormones in weaned piglets after Escherichia coli Lipopolysaccharide (LPS) challenges. A total of 48 crossbred (Duroc x Large; White x Landrace) male pigs weaned at 28 days of age (8.45±0.14 kg) were randomly allotted to one of four treatments by initial BW in a 2×2 factorial design that included a dietary addition of ABP (0 or 500 mg kg-1) and immunological challenge (with LPS or saline). On day 14 and 21, pigs were injected intraperitoneally with either 100 μg kg-1 BW of LPS or an equivalent amount of sterile saline. Blood samples were obtained 3 h after injection for analysis of Tumor Necrosis Factor-α (TNF-α), Prostaglandin E2(PGE2), cortisol, Growth Hormone (GH) and Insulin-like Growth Factor (IGF)-I. The results showed that LPS challenge decreased Average Daily Feed Intake (ADFI) (p<0.05) from 14-21 and day 21 "28. ABP increased ADG (p<0.05) and ADFI (p<0.05) from day 21 "28. An interaction (p<0.05) between LPS challenge and diet was observed for the plasma concentration of TNF-α, PGE2, cortisol, and IGF-I after both LPS challenges such that among LPS-treated pigs, pigs fed the ABP diet were lower for TNF-α (p<0.05) and higher IGF-I (p<0.05) than those receiving the control diet. These results indicated that ABP alters the release of inflammatoiy cytokines that may lead to improved pig performance during an immunological stress. © Medwell Journals, 2014.


Ruan Z.,Nanchang University | Yang Y.,Nanchang University | Zhou Y.,Nanchang University | Wen Y.,Nanchang University | And 10 more authors.
Amino Acids | Year: 2014

This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution 1H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo. © 2014 Springer-Verlag.


Zhang Y.,CAS Institute of Subtropical Agriculture | Zhang Y.,Hunan Engineering and Research Center Animal and Poultry Science | Feng Z.,CAS Institute of Subtropical Agriculture | Feng Z.,Hunan Engineering and Research Center Animal and Poultry Science | And 5 more authors.
Journal of Animal and Veterinary Advances | Year: 2014

Iatric obesity is a growing problem around the world with an increasing prevalence among even infants and young children. The intestinal microbiota have recently been shown to affect the energy balance by influencing both the efficiency of calorie harvest from the diet and how harvested energy is used and stored and is a non-negligible contributor of the formation of obesity. Pig is a good model for use in human nutrition research but few studies have examined the differences among different pig breeds. This study was conducted to compare the diversities of intestinal microbiota among lean Landrace pig and four local Chinese pig breeds with obese phenotype including Bama mini-pig, Huanjiang mini-pig, Ningxiang pig and Lantang pig. These local Chinese pig breeds have obvious differences in genotype and phenotype compared to Landrace pig. The aim of this study was to examine the association between the intestinal microbiota composition in infancy and future obesity using pigs as a model. The results indicated that the local Chinese pig breeds had significantly greater microbiota diversities in the distal intestine than Landrace pig. There are also clear differences in genus that can influence the energy balance and contribute to obesity. The results indicated that the microbiota diversity in the distal intestine in LCBs was significant higher than in LD which challenge the result that the microbiota diversity decrease in obese individuals. Intestinal microbiota do contribute for the obese phenotype but genotype is the main contributor. The higher contents of intestinal microbial populations that show strong energy-harvesting ability may partly contribute to the obese phenotype in local Chinese pigs. The research may help to clarify the mechanism of phenotype diversity in pig breeds and contribute to studies on infant nutrition and obesity. © Medwell Journals, 2014.


PubMed | Purdue University, University of Manitoba, Fujian Aonong biotechnology corporation, Twins Group Co. and 2 more.
Type: Journal Article | Journal: PloS one | Year: 2014

The purpose of this study was to investigate the hypothesis that dietary supplementation with glutamic acid has beneficial effects on growth performance, antioxidant system, intestinal morphology, serum amino acid profile and the gene expression of intestinal amino acid transporters in growing swine fed mold-contaminated feed. Fifteen pigs (LandraceLarge White) with a mean body weight (BW) of 55 kg were randomly divided into control group (basal feed), mycotoxin group (contaminated feed) and glutamate group (2% glutamate+contaminated feed). Compared with control group, mold-contaminated feed decreased average daily gain (ADG) and increased feed conversion rate (FCR). Meanwhile, fed mold-contaminated feed impaired anti-oxidative system and intestinal morphology, as well as modified the serum amino acid profile in growing pigs. However, supplementation with glutamate exhibited potential positive effects on growth performance of pigs fed mold-contaminated feed, ameliorated the imbalance antioxidant system and abnormalities of intestinal structure caused by mycotoxins. In addition, dietary glutamate supplementation to some extent restored changed serum amino acid profile caused by mold-contaminated feed. In conclusion, glutamic acid may be act as a nutritional regulating factor to ameliorate the adverse effects induced by mycotoxins.


Tang Y.,CAS Institute of Subtropical Agriculture | Tan B.,CAS Institute of Subtropical Agriculture | Xiong X.,CAS Institute of Subtropical Agriculture | Li F.,CAS Institute of Subtropical Agriculture | And 5 more authors.
Amino Acids | Year: 2015

Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without l-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy. © 2014 Springer-Verlag Wien.

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