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Wang Z.,Shanghai JiaoTong University | Liang R.,Shanghai JiaoTong University | Xu J.-H.,East China University of Science and Technology | Liu Y.,Hunan Microbiology Institute | Qi H.,Shanghai JiaoTong University
Applied Biochemistry and Biotechnology | Year: 2010

Extractive microbial transformation of benzaldehyde into l-phenylacetylcarbinol (l-PAC) by Saccharomyces cerevisiae (Baker's yeast) has been carried out in a novel polyethylene-glycol-induced cloud-point system (PEG-CPS). The extractive microbial transformation in the PEG-CPS and a downstream process for stripping of the product from the microbial transformation broth with microemulsion extraction are demonstrated. The results indicate that the PEG-CPS maintains the advantage of CPS for in situ extraction of polar product in the microbial transformation. At the same time, the utilization of hydrophilic nonionic surfactant in the PEG-CPS is favorable for stripping of product from the nonionic surfactant in the microbial transformation broth by Winsor I microemulsion extraction. Thus, a closed concept of in situ extraction of polar product in microbial transformation and its downstream process of product recovery are fulfilled at the same time. © 2009 Humana Press.

Wang S.-P.,Hunan Microbiology Institute | Wang S.-P.,CAS Institute of Subtropical Agriculture | Guo Z.-H.,Hunan Microbiology Institute | Liu H.-Z.,Hunan Microbiology Institute | And 12 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

The aim of this study was to develop a convenient method for isolating Lactobacillus without anaerobic equipments. Glass bottles were used to contain the commercial MRS media supplemented with agar powder. After the media was dropped to the bottle, carbon dioxide (CO2) gas was aerated into the bottle, and then the bottle was autoclaved. The mouth of the bottle was sealed with sealing film and the bottle was placed obliquely until the media was solid. A bottle with solid slant medium was made for isolation of Lactobacillus. Using this new method, four different species of Lactobacillus were isolated from pig ilea digesta, which indicated that it is a simple and feasible method for Lactobacillus isolation.

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