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Zhao X.,Tsinghua University | Dong L.,Tsinghua University | Chen L.,Hunan Institute of Nuclear Agricultural Science and Space Breeding | Liu D.,Tsinghua University
Bioresource Technology | Year: 2013

Formiline pretreatment pertains to a biomass fractionation process. In the present work, Formiline-pretreated sugarcane bagasse was hydrolyzed with cellulases by batch and multi-step fed-batch processes at 20% solid loading. For wet pulp, after 144. h incubation with cellulase loading of 10. FPU/g dry solid, fed-batch process obtained ~150. g/L glucose and ~80% glucan conversion, while batch process obtained ~130. g/L glucose with corresponding ~70% glucan conversion. Solid loading could be further increased to 30% for the acetone-dried pulp. By fed-batch hydrolysis of the dried pulp in pH. 4.8 buffer solution, glucose concentration could be 247.3. ±. 1.6. g/L with corresponding 86.1. ±. 0.6% glucan conversion. The enzymatic hydrolyzates could be well converted to ethanol by a subsequent fermentation using Saccharomices cerevisiae with ethanol titer of 60-70. g/L. Batch and fed-batch SSF indicated that Formiline-pretreated substrate showed excellent fermentability. The final ethanol concentration was 80. g/L with corresponding 82.7% of theoretical yield. © 2012 Elsevier Ltd.


Wang K.-Q.,Hunan Institute of Nuclear Agricultural Science and Space Breeding | Xiong X.-Y.,Hunan Agricultural University | Chen J.-P.,Hunan Institute of Nuclear Agricultural Science and Space Breeding | Chen L.,Hunan Institute of Nuclear Agricultural Science and Space Breeding | And 2 more authors.
Biomass and Bioenergy | Year: 2012

It was evaluated the influence of gamma irradiation and steam explosion pretreatment on the components and the water-soluble sugars of rice straw. Compared with the steam explosion pretreated rice straw, cellucose, hemicellucose and lignin for irradiation pretreated rice sample were much more greatly degraded and the relative content of glucose was significantly enhanced from 6.58% to 47.44%. Interestingly, no glucuronide acid was detected in irradiation pretreated rice straw, while glucuronide acid with the content from 8.5 mg/g to 9.2 mg/g was obtained in steam explosion pretreated sample. Followed by enzymatic hydrolysis, higher concentration of reducing sugars (including glucose and xylose) of irradiation pretreated rice sample (90.3 mg/g) was obtained, which was approximately 2.4- and 1.1- fold higher of the unpretreated (37.2 mg/g) and of steam explosion pretreated sample (85.4 mg/g). To further verify the effectiveness of irradiation pretreatment, characterizations of rice straw, corn stalk and bagasse by an integrated process of dilute acid/enzymatic hydrolysis and irradiation pretreatment were also investigated. © 2012 Elsevier Ltd.


Liu Y.,Beijing University of Chemical Technology | Zhou H.,Beijing University of Chemical Technology | Wang S.,Beijing University of Chemical Technology | Wang K.,Hunan Institute of Nuclear Agricultural Science and Space Breeding | Su X.,Hunan Agricultural University
Bioresource Technology | Year: 2015

The effect of γ-irradiation pretreatment was compared with other pretreatment methods including ionic liquids (ILs), 1% HCl, 1% H2SO4, acidic aqueous Ils (AA-ILs), on the bioconversion efficiency of microcrystalline cellulose (MCC) for bioethanol production. The efficiency of MCC pretreatment followed with simultaneous saccharification and fermentation (SSF) was firstly evaluated according to the variations of the irradiation-derived compounds and structure of MCC, as well as yeast growth curve and bioethanol yield. Results showed that the appropriate irradiation dose (891kGy used in our work) could eliminate the negative effect of toxic irradiation-derived compounds on SSF for ethanol bioconversion with the yield value of 67%. Analyses of SEM, FT-IR, reducing sugar and bioethanol yield showed that the efficiency of pretreatment on MCC was ILs≈irradiation pretreatment>AA-ILs pretreatment>1% HCl pretreatment>1% H2SO4 pretreatment. © 2015 Elsevier Ltd.


PubMed | Hunan Institute of Nuclear Agricultural Science and Space Breeding, Hunan Agricultural University and Beijing University of Chemical Technology
Type: | Journal: Bioresource technology | Year: 2015

The effect of -irradiation pretreatment was compared with other pretreatment methods including ionic liquids (ILs), 1% HCl, 1% H2SO4, acidic aqueous Ils (AA-ILs), on the bioconversion efficiency of microcrystalline cellulose (MCC) for bioethanol production. The efficiency of MCC pretreatment followed with simultaneous saccharification and fermentation (SSF) was firstly evaluated according to the variations of the irradiation-derived compounds and structure of MCC, as well as yeast growth curve and bioethanol yield. Results showed that the appropriate irradiation dose (891 kGy used in our work) could eliminate the negative effect of toxic irradiation-derived compounds on SSF for ethanol bioconversion with the yield value of 67%. Analyses of SEM, FT-IR, reducing sugar and bioethanol yield showed that the efficiency of pretreatment on MCC was ILs irradiation pretreatment > AA-ILs pretreatment > 1% HCl pretreatment > 1% H2SO4 pretreatment.

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